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X-ORIGINAL-URL:https://www.med.unc.edu/flowcytometry
X-WR-CALDESC:Events for Flow Cytometry Core Facility
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DTSTART;TZID=America/New_York:20231031T110000
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UID:10000175-1698750000-1698757200@www.med.unc.edu
SUMMARY:Use of High-Parameter Human NK and T cell Spectral Flow Cytometry For an In-Depth Investigation of Immune Cell Variability across Health Donors
DESCRIPTION:Inter-donor immune system variability is well-documented and poses challenges for the selection of normal controls or cell donors in biomarker or cell therapy studies. Spectral flow cytometry has facilitated efficient and extensive characterization of the immune system by enabling analysis of over 40 markers in a single tube. However\, challenges in the development of such assays have so far limited their use to a broad and shallow analysis of several\, distinct lineages. Furthermore\, the lack of biological standards has hampered the assessment of the inevitable impact of spillover spread on biological resolution in very large panels. In this study\, we developed a 38-color spectral flow cytometry panel for an in-depth analysis restricted to NK and T cells\, including markers associated with maturation\, senescence\, activation\, chemokine and inhibitory signaling. Smaller flow cytometry panels with very minimal spillover and spread impact were used as reference to monitor the maintenance of biological resolution\, measured as frequency of populations of interest. The optimized panel was then used to characterize the NK and T cell compartments of N=10 healthy donors of different gender\, age and ethnicity. Through conventional and unsupervised analysis\, we show how inter-donor differences become more pronounced as the depth of cell analysis increases. Longitudinal analysis of N=4 donors showed remarkable immunophenotype stability. Ultimately\, the panel was used for a comprehensive assessment of the impact of commonly used protocols for sample storage and handling of marker detection. This type of analysis can help to explain the plasticity of the immune responses in response to challenge. \nRegister Here: https://forms.office.com/r/kbysCZVM9H \n  \nSeminar Flyer
URL:https://www.med.unc.edu/flowcytometry/event/use-of-high-parameter-human-nk-and-t-cell-spectral-flow-cytometry-for-an-in-depth-investigation-of-immune-cell-variability-across-health-donors/
LOCATION:Pagano Conference Room\, 450 West Drive\, Chapel Hill\, NC\, United States
ORGANIZER;CN="UNC Flow Cytometry":MAILTO:uncflowcyto@med.unc.edu
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BEGIN:VEVENT
DTSTART;TZID=America/New_York:20231113T140000
DTEND;TZID=America/New_York:20231113T150000
DTSTAMP:20260412T115415
CREATED:20230825T202619Z
LAST-MODIFIED:20230825T202620Z
UID:10000173-1699884000-1699887600@www.med.unc.edu
SUMMARY:FlowChat Series
DESCRIPTION:Join us the Second Monday of each Month for discussion of Flow Cytometry Topics
URL:https://www.med.unc.edu/flowcytometry/event/flowchat-series/2023-11-13/
ORGANIZER;CN="UNC Flow Cytometry":MAILTO:uncflowcyto@med.unc.edu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20231211T140000
DTEND;TZID=America/New_York:20231211T150000
DTSTAMP:20260412T115415
CREATED:20230825T202619Z
LAST-MODIFIED:20230825T202620Z
UID:10000174-1702303200-1702306800@www.med.unc.edu
SUMMARY:FlowChat Series
DESCRIPTION:Join us the Second Monday of each Month for discussion of Flow Cytometry Topics
URL:https://www.med.unc.edu/flowcytometry/event/flowchat-series/2023-12-11/
ORGANIZER;CN="UNC Flow Cytometry":MAILTO:uncflowcyto@med.unc.edu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20240820T103000
DTEND;TZID=America/New_York:20240820T120000
DTSTAMP:20260412T115415
CREATED:20240702T145113Z
LAST-MODIFIED:20240702T145113Z
UID:10000176-1724149800-1724155200@www.med.unc.edu
SUMMARY:Cytek Amnis ImageStream Seminar and Machine Learning Applications
DESCRIPTION:Date & Time:\nAugust 20\, 2024\n10:30 AM – 12:00 PM; 1-4:30 pm (1:1 sessions)\nLocation:\nLCCC Pagano Conference Room\nGet Directions\nAgenda:\n\n10:30 AM – 12:00 PM: Presentation by Rob Thacker and Garry Cuneo from Cytek\, showcasing the vast applications of the Cytek Amnis ImageStream technology. Q&A session.\n1:00 PM: Begin one-on-one prearranged sessions. See below for more details\n\nWhy Attend?\n\n\nLearn about the capabilities and advantages of the Cytek Amnis ImageStream Mk II Flow Cytometer.\n\nDiscover how the Machine Learning IDEAS software can enhance your data analysis\, making it more efficient and insightful.\nNetwork with experts and peers in the field\, fostering collaborations and exchanging ideas.\nEngage in one-on-one discussions with Cytek representatives to gain personalized insights into your ongoing or upcoming projects.\n\nTo ensure you don’t miss this opportunity\, please fill out this Microsoft Form to request your one-on-one session.\nAdditional Resources:\nFor more information about the ImageStream technology and its applications within our core facility\, please visit our website. Feel free to explore the attached brochures or contact our Core staff for any queries.\n\nWe look forward to seeing you there!\nUNC Flow Cytometry Core Facility
URL:https://www.med.unc.edu/flowcytometry/event/cytek-amnis-imagestream-seminar-and-machine-learning-applications/
LOCATION:Pagano Conference Room\, 450 West Drive\, Chapel Hill\, NC\, United States
ORGANIZER;CN="UNC Flow Cytometry":MAILTO:uncflowcyto@med.unc.edu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20260115T100000
DTEND;TZID=America/New_York:20260115T120000
DTSTAMP:20260412T115415
CREATED:20251211T213514Z
LAST-MODIFIED:20251211T214020Z
UID:10000177-1768471200-1768478400@www.med.unc.edu
SUMMARY:2026 FlowJo Training
DESCRIPTION:The UNC Flow Cytometry Core is excited to host our BD/FlowJo specialist Dr. Veronica Obregon-Perko\, PhD to present a FlowJo Software Training Seminar.   \nThe seminar will take place: \nThursday January 15th 2026 \nLCCC Atrium \n10-12pm \nOne goal of the seminar is to get hands on experience with FlowJo v11. A sample data set will be provided and if possible we strongly recommend that participants bring their own laptop to follow along with the software presentation. \nThere will also be an opportunity to ask general questions on how to get the most out of your data using FlowJo. \nPlease see the FlowJo Seminar Flyer for details. \nClick here to register for the Seminar \n 
URL:https://www.med.unc.edu/flowcytometry/event/2026-flowjo-training/
LOCATION:LCCC Atrium
ORGANIZER;CN="UNC Flow Cytometry":MAILTO:uncflowcyto@med.unc.edu
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