Fluidigm Helios CyTOF
Anticipated availability at UNC Chapel Hill in the Flow Cytometry Core Facility mid-July 2018.
The Fluidigm Helios CyTOF is a mass cytometer that uniquely combines time-of-flight mass spectrometry with Maxpar metal-labeling technology to enable breakthrough discovery and comprehensive functional profiling applications.
The high purity and choice of metal isotopes provide minimal background noise from signal overlap or endogenous cellular components. Cellular targets in liquid suspension can be labeled with more than 40 metal-tagged reagents, including antibodies, nucleic acid intercalators and analogs, as well as other biochemical ligands.
Targets can be anywhere in the cell, including the surface, cytoplasm, nucleus or internal organelles. Complete profiling allows researchers to investigate a range of applications, including phenotyping, functional activity, apoptosis and cell cycle analysis. Phosphoprotein detection can be used to map the signaling profile of samples.
|Mass range||75–209 amu|
|Abundance sensitivity||0.3% for 159Tb|
|Instrument response||600,000 counts/pg 159Tb|
|Detection limit||350 antibodies/cell|
|Dynamic range||4.5 orders of magnitude|
|Sample introduction||Pneumatic single tube loader with agitation, up to 5 ml volume|
|Peak throughput||2,000 (events/sec)|
|Flow rate||30–45 (µL/min)|
|Replicate sample CV (normalized)||<3%|
|Data File Size (IMD)||0.3 MB/sec/channel|
|Data File Size (FCS)||12.4 bytes/event/channel|
|Data File Size (TXT)||2.4 bytes/event/channel|
Located in Marsico Building – Room B022