Protocols
Cell Cycle:
Propidium Iodide Staining
Analysis of Cellular DNA Content by Flow and Laser Scanning Cytometry
Cell Cycle using Mass Cytometry
Other methods:
DNAse to Prevent Cell Clumping
Amine reactive beads kit for Viability Dye compensation from ThermoFisher
BioLegend Mitospy mitochondria stains in orange and green
RNA Extraction From Sorted Cells
Cell Prep for RNA seq O’Flanagan et al. inBioRxIV
Rainbow Calibration Particles (we encourage all analyzer users to perform their own quality control! We recommend each lab have available some Spherotech 8-peak Rainbow Calibration beads, Cat # RCP-30-5A)