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X-ORIGINAL-URL:https://www.med.unc.edu/flowcytometry
X-WR-CALDESC:Events for Flow Cytometry Core Facility
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DTSTART;TZID=America/New_York:20240820T103000
DTEND;TZID=America/New_York:20240820T120000
DTSTAMP:20260522T192401
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UID:10000176-1724149800-1724155200@www.med.unc.edu
SUMMARY:Cytek Amnis ImageStream Seminar and Machine Learning Applications
DESCRIPTION:Date & Time:\nAugust 20\, 2024\n10:30 AM – 12:00 PM; 1-4:30 pm (1:1 sessions)\nLocation:\nLCCC Pagano Conference Room\nGet Directions\nAgenda:\n\n10:30 AM – 12:00 PM: Presentation by Rob Thacker and Garry Cuneo from Cytek\, showcasing the vast applications of the Cytek Amnis ImageStream technology. Q&A session.\n1:00 PM: Begin one-on-one prearranged sessions. See below for more details\n\nWhy Attend?\n\n\nLearn about the capabilities and advantages of the Cytek Amnis ImageStream Mk II Flow Cytometer.\n\nDiscover how the Machine Learning IDEAS software can enhance your data analysis\, making it more efficient and insightful.\nNetwork with experts and peers in the field\, fostering collaborations and exchanging ideas.\nEngage in one-on-one discussions with Cytek representatives to gain personalized insights into your ongoing or upcoming projects.\n\nTo ensure you don’t miss this opportunity\, please fill out this Microsoft Form to request your one-on-one session.\nAdditional Resources:\nFor more information about the ImageStream technology and its applications within our core facility\, please visit our website. Feel free to explore the attached brochures or contact our Core staff for any queries.\n\nWe look forward to seeing you there!\nUNC Flow Cytometry Core Facility
URL:https://www.med.unc.edu/flowcytometry/event/cytek-amnis-imagestream-seminar-and-machine-learning-applications/
LOCATION:Pagano Conference Room\, 450 West Drive\, Chapel Hill\, NC\, United States
ORGANIZER;CN="UNC Flow Cytometry":MAILTO:uncflowcyto@med.unc.edu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20231031T110000
DTEND;TZID=America/New_York:20231031T130000
DTSTAMP:20260522T192401
CREATED:20231025T172925Z
LAST-MODIFIED:20231025T172925Z
UID:10000175-1698750000-1698757200@www.med.unc.edu
SUMMARY:Use of High-Parameter Human NK and T cell Spectral Flow Cytometry For an In-Depth Investigation of Immune Cell Variability across Health Donors
DESCRIPTION:Inter-donor immune system variability is well-documented and poses challenges for the selection of normal controls or cell donors in biomarker or cell therapy studies. Spectral flow cytometry has facilitated efficient and extensive characterization of the immune system by enabling analysis of over 40 markers in a single tube. However\, challenges in the development of such assays have so far limited their use to a broad and shallow analysis of several\, distinct lineages. Furthermore\, the lack of biological standards has hampered the assessment of the inevitable impact of spillover spread on biological resolution in very large panels. In this study\, we developed a 38-color spectral flow cytometry panel for an in-depth analysis restricted to NK and T cells\, including markers associated with maturation\, senescence\, activation\, chemokine and inhibitory signaling. Smaller flow cytometry panels with very minimal spillover and spread impact were used as reference to monitor the maintenance of biological resolution\, measured as frequency of populations of interest. The optimized panel was then used to characterize the NK and T cell compartments of N=10 healthy donors of different gender\, age and ethnicity. Through conventional and unsupervised analysis\, we show how inter-donor differences become more pronounced as the depth of cell analysis increases. Longitudinal analysis of N=4 donors showed remarkable immunophenotype stability. Ultimately\, the panel was used for a comprehensive assessment of the impact of commonly used protocols for sample storage and handling of marker detection. This type of analysis can help to explain the plasticity of the immune responses in response to challenge. \nRegister Here: https://forms.office.com/r/kbysCZVM9H \n  \nSeminar Flyer
URL:https://www.med.unc.edu/flowcytometry/event/use-of-high-parameter-human-nk-and-t-cell-spectral-flow-cytometry-for-an-in-depth-investigation-of-immune-cell-variability-across-health-donors/
LOCATION:Pagano Conference Room\, 450 West Drive\, Chapel Hill\, NC\, United States
ORGANIZER;CN="UNC Flow Cytometry":MAILTO:uncflowcyto@med.unc.edu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20230907T140000
DTEND;TZID=America/New_York:20230907T160000
DTSTAMP:20260522T192401
CREATED:20230816T164857Z
LAST-MODIFIED:20230831T150751Z
UID:10000169-1694095200-1694102400@www.med.unc.edu
SUMMARY:Full Spectrum Profiling- Cytek Aurora Seminar. Sept 7/2-4 pm/ Pagano
DESCRIPTION:Cytek FSP Seminar 090723 \nThe Flow Cytometry Core Facility Presents Full Spectrum Profiling with the Cytek Aurora. Seminar given by Technical Sales Representative Theresa Persiani. \n  \nWe look forward to seeing you September 7th at 2 pm in LCCC Pagano Conference Room. \nRegister Here \n  \nEmail Ayrianna at ayrianna@med.unc.edu with questions
URL:https://www.med.unc.edu/flowcytometry/event/3409/
LOCATION:Pagano Conference Room\, 450 West Drive\, Chapel Hill\, NC\, United States
ORGANIZER;CN="UNC Flow Cytometry":MAILTO:uncflowcyto@med.unc.edu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20200117T090000
DTEND;TZID=America/New_York:20200117T103000
DTSTAMP:20260522T192401
CREATED:20191114T194315Z
LAST-MODIFIED:20191219T221101Z
UID:10000152-1579251600-1579257000@www.med.unc.edu
SUMMARY:High dimensional cytometry with the Cytek Aurora
DESCRIPTION:Join us for a presentation on the new wave of Spectral Flow Cytometry. Learn what it is and how it is changing the landscape of flow cytometry world wide. \nThis is part of the Research Triangle Cytometry Association (RTCA) series. \nBagels and coffee provided by the Flow Core
URL:https://www.med.unc.edu/flowcytometry/event/high-dimensional-cytometry-with-the-cytek-aurora/
LOCATION:Pagano Conference Room\, 450 West Drive\, Chapel Hill\, NC\, United States
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20191119T120000
DTEND;TZID=America/New_York:20191119T130000
DTSTAMP:20260522T192401
CREATED:20191108T021046Z
LAST-MODIFIED:20191108T021123Z
UID:10000150-1574164800-1574168400@www.med.unc.edu
SUMMARY:Flow Cytometry Best Practices - Lunch and Learn
DESCRIPTION:Ramiro Diz\, Ph.D.\, will present tips on best practices for flow cytometry experimental design\, data collection\, analysis and reporting. \nLunch will be provided by the Flow Core
URL:https://www.med.unc.edu/flowcytometry/event/flow-cytometry-best-practices-lunch-and-learn/
LOCATION:Pagano Conference Room\, 450 West Drive\, Chapel Hill\, NC\, United States
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20181022T101500
DTEND;TZID=America/New_York:20181022T133000
DTSTAMP:20260522T192401
CREATED:20180817T154703Z
LAST-MODIFIED:20181011T190732Z
UID:10000127-1540203300-1540215000@www.med.unc.edu
SUMMARY:Mass Cytometry Research Day
DESCRIPTION:The UNC Flow and Mass Cytometry Core Facilities and the Research Triangle Cytometry Association will host a series of talks on the latest in research using Mass Cytometry. \nMonday October 22 from  10:15 AM – 1:30 PM\, Pagano Conference Room\, LCCC \n  \nSchedule: \n10:15 -10:45 AM – ‘Taking the First Steps of Mass Cytometry’ – Dan Frederick\, PhD\, Fluidigm \n10:45 -11:45 AM –  ‘Learning in vivo: How Data Science and Cytomics Are Connecting Cell Biology and Medicine’ – Jonathan Irish\, Vanderbilt University \n11:45 – 12:00 PM – Grab a lunch \n12:00 – 12:30 PM – ‘Immune profiling of Chlamydia trachomatis infected women’ – Avinash Kollipara\, UNC \n12:30 – 1:30 PM – ‘Deep Immune Profiling using the Maxpar Human Immune Monitoring Panel Kit with Mass Cytometry’ – Bruce Bagwell\, Verity Software House / Greg Stelzer Fluidigm \n  \nIf you plan to stay for lunch\, please register in advance: https://www.eventbrite.com/e/mass-cytometry-research-day-at-unc-registration-50766371584
URL:https://www.med.unc.edu/flowcytometry/event/cytof-research-day/
LOCATION:Pagano Conference Room\, 450 West Drive\, Chapel Hill\, NC\, United States
ORGANIZER;CN="UNC Flow Cytometry":MAILTO:uncflowcyto@med.unc.edu
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