{"id":2246,"date":"2014-04-29T21:12:20","date_gmt":"2014-04-30T01:12:20","guid":{"rendered":"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation-2\/analyzers\/"},"modified":"2026-03-13T12:47:21","modified_gmt":"2026-03-13T16:47:21","slug":"analyzers","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation\/analyzers\/","title":{"rendered":"Analyzers"},"content":{"rendered":"<p>&nbsp;<\/p>\n<figure id=\"attachment_3992\" class=\"thumbnail wp-caption alignnone\" style=\"width: 1290px\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3992 size-full\" src=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/02\/Slide2.jpg\" alt=\"Instrument Decision Tree. A flowchat to help decide which instrument is most appropriate. Attune Nxt, LSRFortessa, Cytek Aurora, and Cytek ImageStream.\" width=\"1280\" height=\"720\" srcset=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/02\/Slide2.jpg 1280w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/02\/Slide2-300x169.jpg 300w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/02\/Slide2-1024x576.jpg 1024w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/02\/Slide2-768x432.jpg 768w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/02\/Slide2-600x338.jpg 600w\" sizes=\"auto, (max-width: 1280px) 100vw, 1280px\" \/><figcaption class=\"caption wp-caption-text\">The UNC Flow Cytometry Core has 4 types of Flow Analyzers. The graphical decision tree helps guide the selection of an appropriate analyzer based on your experimental design. Experiments that require Fluorescent images should use the ImageStream MkII. High parameter and\/or spectral flow experiments should use the Aurora. Lower parameter and\/or classical flow experiments should use the Attune NxT or the Fortessa if a UV excitable dye is included.<\/figcaption><\/figure>\n<som-cell cell-info=\"{&quot;ID&quot;:3876,&quot;post_author&quot;:&quot;103086&quot;,&quot;post_date&quot;:&quot;2025-02-14 12:47:40&quot;,&quot;post_date_gmt&quot;:&quot;2025-02-14 17:47:40&quot;,&quot;post_content&quot;:&quot;&quot;,&quot;post_title&quot;:&quot;Analyzers &#8211; Content Boxes&quot;,&quot;post_excerpt&quot;:&quot;&quot;,&quot;post_status&quot;:&quot;publish&quot;,&quot;comment_status&quot;:&quot;closed&quot;,&quot;ping_status&quot;:&quot;closed&quot;,&quot;post_password&quot;:&quot;&quot;,&quot;post_name&quot;:&quot;analyzers&quot;,&quot;to_ping&quot;:&quot;&quot;,&quot;pinged&quot;:&quot;&quot;,&quot;post_modified&quot;:&quot;2026-04-22 10:43:47&quot;,&quot;post_modified_gmt&quot;:&quot;2026-04-22 14:43:47&quot;,&quot;post_content_filtered&quot;:&quot;&quot;,&quot;post_parent&quot;:0,&quot;guid&quot;:&quot;https:\\\/\\\/www.med.unc.edu\\\/flowcytometry\\\/cell\\\/analyzers\\\/&quot;,&quot;menu_order&quot;:0,&quot;post_type&quot;:&quot;somcell&quot;,&quot;post_mime_type&quot;:&quot;&quot;,&quot;comment_count&quot;:&quot;0&quot;,&quot;filter&quot;:&quot;raw&quot;}\" cell-meta=\"{&quot;_upd_sortorder&quot;:[&quot;1&quot;],&quot;layout&quot;:[&quot;RepeatableBoxes&quot;],&quot;cellInformation&quot;:[&quot;{\\&quot;singleCellBlocks\\&quot;:{},\\&quot;repeatableBoxes\\&quot;:[{\\&quot;includeLink\\&quot;:1,\\&quot;boxImage\\&quot;:\\&quot;https:\\\/\\\/www.med.unc.edu\\\/flowcytometry\\\/wp-content\\\/uploads\\\/sites\\\/1450\\\/2018\\\/06\\\/AttuneNxT.jpg\\&quot;,\\&quot;boxImageAltText\\&quot;:\\&quot;Image of ThermoFisher&#039;s Attune NxT instrument\\&quot;,\\&quot;boxText\\&quot;:\\&quot;ThermoFisher Attune NxT\\&quot;,\\&quot;boxExcerptText\\&quot;:\\&quot;The Thermo Fisher Attune NxT is equipped with a 96\\\/384 well plate Autosampler and 4 lasers (405, 488, 561, and 637nm) and can detect up to 14 fluorescent parameters. Its acquisition software is Attune NxT.\\\\n\\\\nSamples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on both Attune 1 and Attune 2.\\&quot;,\\&quot;boxUrl\\&quot;:\\&quot;https:\\\/\\\/www.med.unc.edu\\\/flowcytometry\\\/instrumentation\\\/analyzers\\\/thermo-fisher-attune-nxt\\\/\\&quot;,\\&quot;useRichTextEditor\\&quot;:\\&quot;true\\&quot;,\\&quot;boxRichText\\&quot;:\\&quot;&lt;p&gt;The Thermo Fisher Attune NxT is equipped with a 96\\\/384 well plate Autosampler and 4 lasers (405, 488, 561, and 637nm) and can detect up to 14 fluorescent parameters. Its acquisition software is Attune NxT.&lt;\\\/p&gt;&lt;p&gt;&lt;\\\/p&gt;&lt;p&gt;Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on both Attune 1 and Attune 2.&lt;\\\/p&gt;\\&quot;},{\\&quot;includeLink\\&quot;:1,\\&quot;boxImage\\&quot;:\\&quot;https:\\\/\\\/www.med.unc.edu\\\/flowcytometry\\\/wp-content\\\/uploads\\\/sites\\\/1450\\\/2018\\\/06\\\/lsrfortessa.png\\&quot;,\\&quot;boxImageAltText\\&quot;:\\&quot;Image of BD&#039;s LSRFortessa instrument\\&quot;,\\&quot;boxText\\&quot;:\\&quot;BD LSRFortessa\\&quot;,\\&quot;boxExcerptText\\&quot;:\\&quot;The Becton Dickinson LSRFortessa is equipped with 5 lasers (355, 405, 488, 561, and 640nm) and can detect up to 18 fluorescent parameters. Its acquisition software is FACSDiva.\\\\n\\\\nSamples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the LSRFortessa.\\&quot;,\\&quot;boxUrl\\&quot;:\\&quot;https:\\\/\\\/www.med.unc.edu\\\/flowcytometry\\\/instrumentation\\\/analyzers\\\/bdfortessa\\&quot;,\\&quot;useRichTextEditor\\&quot;:\\&quot;true\\&quot;,\\&quot;boxRichText\\&quot;:\\&quot;&lt;p&gt;The Becton Dickinson LSRFortessa is equipped with 5 lasers (355, 405, 488, 561, and 640nm) and can detect up to 18 fluorescent parameters. Its acquisition software is FACSDiva.&lt;\\\/p&gt;&lt;p&gt;&lt;\\\/p&gt;&lt;p&gt;Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the LSRFortessa.&lt;\\\/p&gt;\\&quot;},{\\&quot;includeLink\\&quot;:1,\\&quot;boxImage\\&quot;:\\&quot;https:\\\/\\\/www.med.unc.edu\\\/flowcytometry\\\/wp-content\\\/uploads\\\/sites\\\/1450\\\/2017\\\/06\\\/Cytek-Aurora.png\\&quot;,\\&quot;boxImageAltText\\&quot;:\\&quot;Image of Cytek&#039;s Aurora instrument\\&quot;,\\&quot;boxText\\&quot;:\\&quot;Cytek Aurora\\&quot;,\\&quot;boxExcerptText\\&quot;:\\&quot;The Cytek Aurora is equipped with 5 lasers (355nm, 405nm, 488nm, 561nm and 640nm), 3 scatter channels (FSC, SSC-Blue, and SSC-Violet) and has 64 fluorescence channels. This is a spectral cytometer. Its acquisition and analysis software is SpectroFlo\\u00ae Software. It is equipped with a plate reader for 96 and 384 well plates.\\\\n\\\\nSamples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the Aurora.\\&quot;,\\&quot;boxUrl\\&quot;:\\&quot;https:\\\/\\\/www.med.unc.edu\\\/flowcytometry\\\/instrumentation\\\/analyzers\\\/analyzers-cytek-aurora\\\/\\&quot;,\\&quot;useRichTextEditor\\&quot;:\\&quot;true\\&quot;,\\&quot;boxRichText\\&quot;:\\&quot;&lt;p&gt;The Cytek Aurora is equipped with 5 lasers (355nm, 405nm, 488nm, 561nm and 640nm), 3 scatter channels (FSC, SSC-Blue, and SSC-Violet) and has 64 fluorescence channels. This is a spectral cytometer. Its acquisition and analysis software is SpectroFlo\\u00ae Software. It is equipped with a plate reader for 96 and 384 well plates.&lt;\\\/p&gt;&lt;p&gt;&lt;\\\/p&gt;&lt;p&gt;Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the Aurora.&lt;\\\/p&gt;\\&quot;},{\\&quot;includeLink\\&quot;:1,\\&quot;boxImage\\&quot;:\\&quot;https:\\\/\\\/www.med.unc.edu\\\/flowcytometry\\\/wp-content\\\/uploads\\\/sites\\\/1450\\\/2017\\\/06\\\/ImageStream-MkII.jpg\\&quot;,\\&quot;boxImageAltText\\&quot;:\\&quot;Cytek&#039;s ImageStream MkII instrument\\&quot;,\\&quot;boxText\\&quot;:\\&quot;Cytek Amins ImageStream MkII\\&quot;,\\&quot;boxExcerptText\\&quot;:\\&quot;The Cytek Amnis ImageStreamX Mark II is equipped with 3 colinear lasers (405, 488, and 642) and can detect up to 12 parameters (brightfield, darkfield [SSC] and up to 9 fluorescence parameters). Its acquisition software is INSPIRE.\\\\n\\\\nSamples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the ImageStreamX Mark II.\\&quot;,\\&quot;boxUrl\\&quot;:\\&quot;https:\\\/\\\/www.med.unc.edu\\\/flowcytometry\\\/instrumentation\\\/analyzers\\\/unc-amnis-imagestreamx-mark-ii\\\/\\&quot;,\\&quot;useRichTextEditor\\&quot;:\\&quot;true\\&quot;,\\&quot;boxRichText\\&quot;:\\&quot;&lt;p&gt;The Cytek Amnis ImageStreamX Mark II is equipped with 3 colinear lasers (405, 488, and 642) and can detect up to 12 parameters (brightfield, darkfield [SSC] and up to 9 fluorescence parameters). Its acquisition software is INSPIRE.&lt;\\\/p&gt;&lt;p&gt;&lt;\\\/p&gt;&lt;p&gt;Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the ImageStreamX Mark II.&lt;\\\/p&gt;\\&quot;}],\\&quot;boxCustomStyles\\&quot;:{\\&quot;textAlign\\&quot;:\\&quot;left\\&quot;,\\&quot;includeImage\\&quot;:1,\\&quot;includeImagePadding\\&quot;:1,\\&quot;imageAspectRatio\\&quot;:\\&quot;3\\\/2\\&quot;,\\&quot;includeLink\\&quot;:1,\\&quot;backgroundColor\\&quot;:\\&quot;lightBlue\\&quot;,\\&quot;columnLayout\\&quot;:\\&quot;2\\&quot;},\\&quot;boxType\\&quot;:\\&quot;imageBoxes\\&quot;,\\&quot;boxStyleOption\\&quot;:\\&quot;content-boxes\\&quot;}&quot;],&quot;apiCallInformation&quot;:[&quot;{}&quot;]}\"><\/som-cell>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp;<\/p>\n","protected":false},"author":79063,"featured_media":0,"parent":2226,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2246","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2246","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/users\/79063"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/comments?post=2246"}],"version-history":[{"count":7,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2246\/revisions"}],"predecessor-version":[{"id":4528,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2246\/revisions\/4528"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2226"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/media?parent=2246"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}