{"id":2298,"date":"2014-04-29T21:15:00","date_gmt":"2014-04-30T01:15:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation-2\/analyzers\/copy_of_becton-dickinson-lsr-ii\/"},"modified":"2024-12-13T11:47:50","modified_gmt":"2024-12-13T16:47:50","slug":"copy_of_becton-dickinson-lsr-ii","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation\/analyzers\/copy_of_becton-dickinson-lsr-ii\/","title":{"rendered":"Becton Dickinson LSRFortessa"},"content":{"rendered":"<p style=\"text-align: justify\"><span style=\"color: #000000\">The Becton Dickinson LSRFortessa is equipped with 5 lasers (355, 405, 488, 561, and 640nm) and can detect up to 18 fluorescent parameters. Its acquisition software is FACSDiva 8.0.1.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"color: #000000\">Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the LSRFortessa.<\/span><\/p>\n<p style=\"text-align: center\"><strong style=\"text-align: center;line-height: 1.43em\"><span class=\"normalSizeFont\" style=\"color: #ff0000\">We require a copy of your SCHEDULE F for any biohazardous materials brought into the core.<\/span><\/strong><\/p>\n<p style=\"text-align: justify\"><span style=\"color: #000000;line-height: 1.43em\">The investigator must comply with the following rules:<\/span><\/p>\n<ul>\n<li><span style=\"color: #000000\"><strong>PPE <\/strong>&#8211; Lab coat, gloves, eye protection and closed toe shoes are to be used at all times.<\/span><\/li>\n<li><span style=\"color: #000000\"><strong>Transport <\/strong>&#8211; Samples are to be transported to and from room B021C in an approved biosafety transport container.<\/span><\/li>\n<li><span style=\"color: #000000\"><strong>Spills <\/strong>&#8211; If any sample is spilled in the room it is to be reported to Facility staff and cleaned up using bleach.<\/span><\/li>\n<li><span style=\"color: #000000\"><strong>Disposal <\/strong>&#8211; No samples or other contaminated materials are to be disposed of down the sink.<\/span><\/li>\n<\/ul>\n<p><span style=\"color: #000000\"> Any contaminated materials should be discarded into the biohazard trash.<br \/>\nLeft over samples are to be returned to the user&#8217;s lab using the approved transport container.<\/span><\/p>\n<ul>\n<li><span style=\"color: #000000\"><strong>Cleaning <\/strong>&#8211; When finished running your samples, follow the cleaning procedure posted on the instrument.<\/span><\/li>\n<\/ul>\n<p style=\"text-align: justify\"><a style=\"line-height: 1.43em\" href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2018\/06\/lsrfortessa-optical-layout.pdf\">Optical Layout<\/a><\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2022\/05\/UT-SOP003.3-Becton-Dickson-LSRFortessa-User-Training.pdf\" target=\"_blank\" rel=\"noopener\">Becton Dickson LSR Fortessa User Training<\/a><\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2024\/12\/Fortessa-Flowchart.pdf\" target=\"_blank\" rel=\"noopener\">Fortessa Troubleshooting Flowchart<\/a><\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2024\/12\/Fortessa-QuickStart-Guidepdf.pdf\" target=\"_blank\" rel=\"noopener\">Fortessa QuickStart Guide<\/a><\/p>\n<div align=\"center\">\n<p><img decoding=\"async\" class=\"image-inline\" title=\"LSRFortessa\" src=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2018\/06\/lsrfortessa.png\" alt=\"LSRFortessa\" \/><\/p>\n<p style=\"text-align: left\"><span style=\"line-height: 1.43em;text-align: justify;color: #000000\">Located in Marsico Building &#8211; Room<\/span><span style=\"color: #000000;line-height: 1.43em;text-align: justify\"> <strong>B021C<\/strong> <\/span><span style=\"line-height: 1.43em;text-align: justify;color: #000000\">(BSL2 Suite)<\/span><\/p>\n<p style=\"text-align: left\"><a href=\"\/flowcytometry\/core-access-scheduling-2\/training-1\">Training<\/a><\/p>\n<p style=\"text-align: left\"><a href=\"https:\/\/my.ilabsolutions.com\/account\/login\">Scheduling<\/a><\/p>\n<p style=\"text-align: left\"><span style=\"color: #000000\">\u00a0<\/span><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Becton Dickinson LSRFortessa is equipped with 5 lasers (355, 405, 488, 561, and 640nm) and can detect up to 18 fluorescent parameters. Its acquisition software is FACSDiva 8.0.1. Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the LSRFortessa. We require a &hellip; <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation\/analyzers\/copy_of_becton-dickinson-lsr-ii\/\" aria-label=\"Read more about Becton Dickinson LSRFortessa\">Read more<\/a><\/p>\n","protected":false},"author":79063,"featured_media":0,"parent":2246,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2298","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Becton Dickinson LSRFortessa - Flow Cytometry Core Facility<\/title>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Becton Dickinson LSRFortessa - Flow Cytometry Core Facility\" \/>\n<meta property=\"og:description\" content=\"The Becton Dickinson LSRFortessa is equipped with 5 lasers (355, 405, 488, 561, and 640nm) and can detect up to 18 fluorescent parameters. Its acquisition software is FACSDiva 8.0.1. Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the LSRFortessa. 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