{"id":2303,"date":"2017-05-17T20:05:00","date_gmt":"2017-05-18T00:05:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation-2\/analyzers\/thermo-fisher-attune-nxt\/"},"modified":"2026-02-03T14:37:41","modified_gmt":"2026-02-03T19:37:41","slug":"thermo-fisher-attune-nxt","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation\/analyzers\/thermo-fisher-attune-nxt\/","title":{"rendered":"ThermoFisher Attune NxT"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-2249 size-large\" src=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2018\/06\/AttuneNxT-1024x502.jpg\" alt=\"Attune NxT\" width=\"1024\" height=\"502\" srcset=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2018\/06\/AttuneNxT-1024x502.jpg 1024w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2018\/06\/AttuneNxT-150x74.jpg 150w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2018\/06\/AttuneNxT-300x147.jpg 300w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2018\/06\/AttuneNxT-768x377.jpg 768w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2018\/06\/AttuneNxT.jpg 1515w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>The ThermoFisher Attune NxT is equipped with a 96\/384 well plate Autosampler, 4 lasers (405, 488, 561, and 637nm) and can detect up to 14 fluorescent parameters. Its acquisition software is Attune NxT 7.1.0.<\/p>\n<p>Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on both Attune 1 (<strong>B018<\/strong>) and Attune 2 (<strong>B021A<\/strong>).<\/p>\n\n<ul class=\"nav nav-tabs oscitas-bootstrap-container yourcustomclass\" id=\"unc-tab-0\" role=\"tablist\">\n\n    \n        \n            <li class=\"active oscitas-bootstrap-container nav-tab-list-item\" role=\"tab\" aria-selected=\"true\">\n\n                    \n            <a role=\"button\" class=\" oscitas-bootstrap-container nav-tab-button\" href=\"#tabs-0-0\" data-toggle=\"tab\">\n\n            Optical Layout\n            <\/a>\n\n        <\/li>\n\n    \n        \n            <li role=\"tab\" class=\"nav-tab-list-item\" aria-selected=\"false\">\n\n                    \n            <a role=\"button\" class=\" oscitas-bootstrap-container nav-tab-button\" href=\"#tabs-0-1\" data-toggle=\"tab\">\n\n            User Training Guide\n            <\/a>\n\n        <\/li>\n\n    \n        \n            <li role=\"tab\" class=\"nav-tab-list-item\" aria-selected=\"false\">\n\n                    \n            <a role=\"button\" class=\" oscitas-bootstrap-container nav-tab-button\" href=\"#tabs-0-2\" data-toggle=\"tab\">\n\n            Quick Start and Troubleshooting\n            <\/a>\n\n        <\/li>\n\n    \n<\/ul>\n\n<div class=\"tab-content\">\n\n    \n        <div role=\"tabpanel\" class=\"tab-pane active oscitas-bootstrap-container\" id=\"tabs-0-0\">\n        \n            \n<p>The FlowCore has two Attune NxT instruments. The Optical layouts remain the same between both instruments, though the voltage settings may be different. View the optical laouts with Core-generated starting voltages below:<\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/10\/Attune-1-Voltage-09-25-25-revised.pdf\" target=\"_blank\" rel=\"noopener\">Attune V6 configuration (Attune 1 Voltages)<\/a> Rev. 9-25-2025<\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/10\/Attune-2-Voltage-09-25-25-revised.pdf\" target=\"_blank\" rel=\"noopener\">Attune V6 configuration (Attune 2 Voltages)<\/a> Rev. 9-25-2025<\/p>\n<p>For Panel Design help, Contact the Flow Core and\/or use <a href=\"https:\/\/easypanel-v2.flow-cytometry.net\/login\" rel=\"noopener\">EasyPanel<\/a>.<\/p>\n<p>The Attune uses acoustic focusing to be able to acquire samples at high speeds (12.5-1000 uL\/min). To ensure the best flow, concentrate your cells appropiately: <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2020\/08\/Attune-NxT-cell-concentrations.pdf\" target=\"_blank\" rel=\"noopener\">Attune NxT cell concentrations<\/a><\/p>\n\n        <\/div>\n\n    \n        <div role=\"tabpanel\" class=\"tab-pane  oscitas-bootstrap-container\" id=\"tabs-0-1\">\n        \n            \n<p><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2022\/05\/UT-SOP007.5-Thermo-Fisher-Attune-NxT-User-Training.pdf\">UT-SOP007.5-Thermo-Fisher-Attune-NxT-User-Training<\/a>. Last Updated 01\/07\/2026.<\/p>\n<p>The instrument can take tubes (1.5 mL or 5 mL) or plates. See Validated Plates here: <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2018\/06\/validated-plates-for-attune-nxt-autosampler.pdf\" target=\"_blank\" rel=\"noopener\">Validated Plates<\/a><\/p>\n\n        <\/div>\n\n    \n        <div role=\"tabpanel\" class=\"tab-pane  oscitas-bootstrap-container\" id=\"tabs-0-2\">\n        \n            \n<p><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/03\/Attune-NxT-Startup-Shutdown-poster.pdf\" target=\"_blank\" rel=\"noopener\">Attune NxT Startup Shutdown CheatSheet<\/a><\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2024\/12\/Attune-NxT-QuickStart-Guide.pdf\" target=\"_blank\" rel=\"noopener\">Attune NxT QuickStart Guide<\/a><\/p>\n\n        <\/div>\n\n    \n<\/div>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; The ThermoFisher Attune NxT is equipped with a 96\/384 well plate Autosampler, 4 lasers (405, 488, 561, and 637nm) and can detect up to 14 fluorescent parameters. Its acquisition software is Attune NxT 7.1.0. Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run &hellip; <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation\/analyzers\/thermo-fisher-attune-nxt\/\" aria-label=\"Read more about ThermoFisher Attune NxT\">Read more<\/a><\/p>\n","protected":false},"author":79063,"featured_media":0,"parent":2246,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2303","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2303","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/users\/79063"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/comments?post=2303"}],"version-history":[{"count":13,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2303\/revisions"}],"predecessor-version":[{"id":4372,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2303\/revisions\/4372"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2246"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/media?parent=2303"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}