{"id":2304,"date":"2015-04-29T18:55:00","date_gmt":"2015-04-29T22:55:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation-2\/analyzers\/unc-amnis-imagestreamx-mark-ii\/"},"modified":"2026-04-17T09:15:11","modified_gmt":"2026-04-17T13:15:11","slug":"unc-amnis-imagestreamx-mark-ii","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation\/analyzers\/unc-amnis-imagestreamx-mark-ii\/","title":{"rendered":"Cytek ImageStreamX Mk II"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-3875 size-medium\" src=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2017\/06\/ImageStream-MkII-300x300.jpg\" alt=\"ImageStream MkII instrument\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2017\/06\/ImageStream-MkII-300x300.jpg 300w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2017\/06\/ImageStream-MkII-1024x1024.jpg 1024w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2017\/06\/ImageStream-MkII-150x150.jpg 150w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2017\/06\/ImageStream-MkII-768x768.jpg 768w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2017\/06\/ImageStream-MkII-600x600.jpg 600w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2017\/06\/ImageStream-MkII-400x400.jpg 400w, https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2017\/06\/ImageStream-MkII.jpg 1500w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: justify\"><span style=\"color: #000000\">The Cytek ImageStreamX Mk II is equipped with 3 collinear lasers (405, 488, and 642) and can detect up to 12 parameters (brightfield, darkfield [SSC] and up to 9 fluorescence parameters). Its acquisition software is <strong>INSPIRE<\/strong>.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"color: #000000;line-height: 1.43em\">Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the ImageStreamX Mark II.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"color: #000000\">Acquired data can be analyzed on the ImageStreamX workstation using the <strong>IDEAS software.<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n\n<ul class=\"nav nav-tabs oscitas-bootstrap-container yourcustomclass\" id=\"unc-tab-0\" role=\"tablist\">\n\n    \n        \n            <li class=\"active oscitas-bootstrap-container nav-tab-list-item\" role=\"tab\" aria-selected=\"true\">\n\n                    \n            <a role=\"button\" class=\" oscitas-bootstrap-container nav-tab-button\" href=\"#tabs-0-0\" data-toggle=\"tab\">\n\n            Optical Layout\n            <\/a>\n\n        <\/li>\n\n    \n        \n            <li role=\"tab\" class=\"nav-tab-list-item\" aria-selected=\"false\">\n\n                    \n            <a role=\"button\" class=\" oscitas-bootstrap-container nav-tab-button\" href=\"#tabs-0-1\" data-toggle=\"tab\">\n\n            User Guide\n            <\/a>\n\n        <\/li>\n\n    \n<\/ul>\n\n<div class=\"tab-content\">\n\n    \n        <div role=\"tabpanel\" class=\"tab-pane active oscitas-bootstrap-container\" id=\"tabs-0-0\">\n        \n             See the optical layout here: <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/10\/ImageStreamX-Optical-Layout.pdf\">Optical Layout<\/a> \n        <\/div>\n\n    \n        <div role=\"tabpanel\" class=\"tab-pane  oscitas-bootstrap-container\" id=\"tabs-0-1\">\n        \n             User Guide: <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2026\/04\/UT-SOP004.3-Cytek-Amnis-ISX-MkII-INSPIRE-User-Training.pdf\">ImageStream User Guide<\/a><\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2018\/06\/imagestreamx-sample-preparation.pdf\">Sample Prep Guide<\/a><\/p>\n<p>Resources Folder in Teams Page: <a href=\"https:\/\/adminliveunc.sharepoint.com\/:f:\/r\/sites\/FlowCoreUserGroup\/Shared%20Documents\/General\/ImageStream%20Resources?csf=1&amp;web=1&amp;e=QE3zRB\">ImageStream Resources<\/a><br \/>\n\n        <\/div>\n\n    \n<\/div>\n<p>Located in Marsico Building &#8211; Room <strong>B021C<\/strong> (BSL2 Suite)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Cytek ImageStreamX Mk II is equipped with 3 collinear lasers (405, 488, and 642) and can detect up to 12 parameters (brightfield, darkfield [SSC] and up to 9 fluorescence parameters). Its acquisition software is INSPIRE. Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be &hellip; <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation\/analyzers\/unc-amnis-imagestreamx-mark-ii\/\" aria-label=\"Read more about Cytek ImageStreamX Mk II\">Read more<\/a><\/p>\n","protected":false},"author":103086,"featured_media":0,"parent":2246,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2304","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2304","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/users\/103086"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/comments?post=2304"}],"version-history":[{"count":11,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2304\/revisions"}],"predecessor-version":[{"id":4589,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2304\/revisions\/4589"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2246"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/media?parent=2304"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}