{"id":3328,"date":"2022-11-21T12:19:22","date_gmt":"2022-11-21T17:19:22","guid":{"rendered":"https:\/\/www.med.unc.edu\/flowcytometry\/?page_id=3328"},"modified":"2026-03-17T10:45:10","modified_gmt":"2026-03-17T14:45:10","slug":"facsmelody-bsl-1","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation\/sorters\/facsmelody-bsl-1\/","title":{"rendered":"FACSMelody (BSL-1)"},"content":{"rendered":"<p>The Becton Dickinson FACSMelody is equipped with 3 lasers (405, 488, and 561nm) and can detect up to 8 fluorescent parameters. Its acquisition software is FACSChorus.<\/p>\n<p style=\"text-align: center\"><strong><span style=\"color: #000000\"><span class=\"largeFont\">Trained users can operate this sorter<\/span>.<\/span><span style=\"color: #ff0000\"><br \/>\n<\/span><\/strong><a title=\"\" href=\"https:\/\/www.med.unc.edu\/flowcytometry\/core-access-scheduling-2\/training-1\/\" target=\"_self\" rel=\"noopener\">For more information<\/a><\/p>\n<p style=\"text-align: center\"><span style=\"color: #000000;line-height: 1.43em\">Samples containing <\/span><strong style=\"line-height: 1.43em\">viable BSL-2<\/strong> <strong style=\"line-height: 1.43em\">agents <\/strong><span style=\"color: #000000;line-height: 1.43em\">(or samples that could contain such agents but whose status is unknown) <\/span><span style=\"color: #000000\"><strong style=\"line-height: 1.43em\">CANNOT<\/strong><\/span><span style=\"color: #000000;line-height: 1.43em\"> be sorted on the FACSMelody.\u00a0<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-3330\" src=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2022\/11\/Melody.jpeg\" alt=\"BD FACSMelody sorter. \" width=\"400\" height=\"300\" \/><\/p>\n<p>Located in Marsico Building \u2013 Room\u00a0<strong>B020<\/strong><\/p>\n\n<ul class=\"nav nav-tabs oscitas-bootstrap-container yourcustomclass\" id=\"unc-tab-0\" role=\"tablist\">\n\n    \n        \n            <li class=\"active oscitas-bootstrap-container nav-tab-list-item\" role=\"tab\" aria-selected=\"true\">\n\n                    \n            <a role=\"button\" class=\" oscitas-bootstrap-container nav-tab-button\" href=\"#tabs-0-0\" data-toggle=\"tab\">\n\n            Optical Layout\n            <\/a>\n\n        <\/li>\n\n    \n        \n            <li role=\"tab\" class=\"nav-tab-list-item\" aria-selected=\"false\">\n\n                    \n            <a role=\"button\" class=\" oscitas-bootstrap-container nav-tab-button\" href=\"#tabs-0-1\" data-toggle=\"tab\">\n\n            User Training Guide\n            <\/a>\n\n        <\/li>\n\n    \n<\/ul>\n\n<div class=\"tab-content\">\n\n    \n        <div role=\"tabpanel\" class=\"tab-pane active oscitas-bootstrap-container\" id=\"tabs-0-0\">\n        \n            \nThe FACSMelody has 3 lasers and 8 detectors.\u00a0 Despite the limited detectors the FACSMelody has proved to be versatile and has been successfully been used to detect some dyes traditionally excited by a 633nm (red) laser. Contact the Flow Core for options to make the best use of your panel.<\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2022\/11\/FACSMelody-Optical-Layout.pdf\">FACSMelody Optical Layout<\/a><\/p>\n<p>For Panel Design help, Contact the Flow Core and\/or use <a href=\"https:\/\/easypanel-v2.flow-cytometry.net\/login\" target=\"_blank\" rel=\"noopener\">EasyPanel<\/a>.<br \/>\n\n        <\/div>\n\n    \n        <div role=\"tabpanel\" class=\"tab-pane  oscitas-bootstrap-container\" id=\"tabs-0-1\">\n        \n            \n<a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2025\/10\/Melody-Training-Guide.pdf\">FACSMelody-Training-Guide<\/a>\u00a0Last Updated 08\/21\/2025.<br \/>\n\n        <\/div>\n\n    \n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Becton Dickinson FACSMelody is equipped with 3 lasers (405, 488, and 561nm) and can detect up to 8 fluorescent parameters. Its acquisition software is FACSChorus. Trained users can operate this sorter. For more information Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) CANNOT be sorted &hellip; <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation\/sorters\/facsmelody-bsl-1\/\" aria-label=\"Read more about FACSMelody (BSL-1)\">Read more<\/a><\/p>\n","protected":false},"author":103086,"featured_media":0,"parent":2257,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-3328","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/3328","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/users\/103086"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/comments?post=3328"}],"version-history":[{"count":8,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/3328\/revisions"}],"predecessor-version":[{"id":4534,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/3328\/revisions\/4534"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2257"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/media?parent=3328"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}