{"id":3672,"date":"2025-02-05T11:48:03","date_gmt":"2025-02-05T16:48:03","guid":{"rendered":"https:\/\/www.med.unc.edu\/flowcytometry\/?page_id=3672"},"modified":"2026-04-02T09:40:55","modified_gmt":"2026-04-02T13:40:55","slug":"staff-assisted","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/flowcytometry\/services\/staff-assisted\/","title":{"rendered":"Staff-Assisted Acquisition &amp; Sorting"},"content":{"rendered":"<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-12 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\n<a class=\"btn btn-primary btn-sm btn-block  oscitas-bootstrap-container\" target=\"_self\" href=\"#acquisition\">Staff-Assisted Acquisition<\/a>\n<\/div>\n<div class=\"col-lg-4 col-md-12 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\n<a class=\"btn btn-primary btn-sm btn-block  oscitas-bootstrap-container\" target=\"_self\" href=\"#sorting\">Staff-Assisted Sorting<\/a>\n<\/div>\n<div class=\"col-lg-4 col-md-12 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\n<a class=\"btn btn-primary btn-sm btn-block  oscitas-bootstrap-container\" target=\"_self\" href=\"https:\/\/uncch.ilab.agilent.com\/service_center\/3267\/?tab=services\">Request Service<\/a>\n<\/div>\n<\/div>\n<h2>Expectations for Staff-Assisted Workflow:<\/h2>\n<ul>\n<li>Staff check submissions daily, however, availability depends on instrument AND staff time. <em>Notice of 1 week is preferred;<\/em> however, times\/dates are not guaranteed. Staff do their best to meet your preferred time and date.<\/li>\n<li>Responses are expected within 48 hours (about 2 days). Instruments are used by independent users and staff, ALL TIMES GIVEN ARE TENTATIVE until the appointment is confirmed in iLabs.<\/li>\n<li><strong>If any changes occur between form submission and the appointment, make sure to update the form AND email staff so any adjustments can be made if necessary.<\/strong><\/li>\n<li><strong><span style=\"color: #000000;\">Required reading for Staff-Assisted Sorting<\/span><\/strong>: Sorting Guides:\u00a0<a style=\"background-color: #ffffff;\" href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/1450\/2019\/04\/Practical-Cell-Sorting-2019-2.pdf\" target=\"_blank\" rel=\"noopener\">Practical Cell Sorting<\/a>\u00a0and the\u00a0<a style=\"background-color: #ffffff;\" href=\"https:\/\/www.med.unc.edu\/flowcytometry\/wp-content\/uploads\/sites\/744\/2022\/05\/LPP-SOP010.2-Sorting-Requirements-at-UNC-Flow-Cytometry-Core-Facility-1.pdf\" target=\"_blank\" rel=\"noopener\">Flow Facility Sorting SOP<\/a>.<\/li>\n<\/ul>\n<hr \/>\n<h2 id=\"acquisition\">Staff-Assisted Acquisition<\/h2>\n<p>The UNC Flow Core offers acquisition services for <strong>researcher prepared samples<\/strong> on all of our flow <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/instrumentation\/analyzers\/\">analyzers<\/a>. Staff is happy to consult on panel and experimental design to ensure successful data acquisition.<\/p>\n<p>Staff-Assisted Flow data acquisition requests can be made through the <a href=\"https:\/\/uncch.ilab.agilent.com\/sc\/3267\/unc-flow-cytometry-core-facility\/?tab=equipment\">UNC Flow Cytometry Core iLabs website<\/a> by following the following steps:<\/p>\n<ol>\n<li><strong>Initiate an <a href=\"https:\/\/uncch.ilab.agilent.com\/service_item\/new\/3267?spt_id=27570\" target=\"_blank\" rel=\"noopener\">Acquisition Request. <\/a><\/strong>Fill the form thoroughly, accurately, and completely.<\/li>\n<li>Staff will reach out once the form is submitted to confirm a time and date.<\/li>\n<li>Arrive at the Flow Core at the agreed upon time.<\/li>\n<li>For new projects staff will ask you to confirm instrument settings at the start of the acquisition.<\/li>\n<li>Following data acquisition staff will upload data to OneDrive and send you a link to the folder via email. This link will also be available via the iLabs request once the request is completed.<\/li>\n<\/ol>\n<p>If you plan to have on-going experiments that will utilize staff-assisted acquisition we recommend requesting a free <a href=\"https:\/\/uncch.ilab.agilent.com\/sc\/3267\/unc-flow-cytometry-core-facility\/?tab=services\">consultation<\/a> with the UNC Flow Core Staff to discuss best-practices for sample preparation, and to develop a plan for data management.<\/p>\n<p>The UNC Flow Cytometry core does not currently offer sample preparation services.\u00a0 If you have interest in collaborating with the UNC Flow Cytometry Core on a project and would like support in sample preparation or data analysis please contact <a href=\"mailto:ramiro_diz@med.unc.edu\">Ramiro Diz.<\/a><\/p>\n<hr \/>\n<h2 id=\"sorting\">Staff-Assisted Sorting<\/h2>\n<p>The UNC Flow Core Staff runs our BSL2+ sorters. For safety reasons BSL2 and BSL2+ samples can only be run by staff on the UNC Flow Core&#8217;s dedicated BSL2+ sorters. The UNC Flow Core sorters can accommodate a range of particles including, but not limited to: lymphocytes, tumor digests, cell lines, bacteria, yeast, tissue digests, virus-infected cells, and beads. Our sorters can simultaneously sort up to 4 (Aria II) or 6 (Symphony S6) populations of cells. Cells can be collected as bulk cell populations, or single cells. We have the capability to sort BSL1 thru BSL2+ samples, but we do not currently have the capability to sort BSL3 and above items. Our sorters use nozzles ranging from 70-100 um.<\/p>\n<p>Staff-Assisted Sorting requests can be made through the <a href=\"https:\/\/uncch.ilab.agilent.com\/sc\/3267\/unc-flow-cytometry-core-facility\/?tab=equipment\">UNC Flow Cytometry Core iLabs website <\/a>by following the following steps:<\/p>\n<ol>\n<li><strong>Initiate\u00a0<a href=\"https:\/\/uncch.ilab.agilent.com\/service_item\/new\/3267?spt_id=2794\" target=\"_blank\" rel=\"noopener\">Sorting Request<\/a>. <\/strong>Fill form thoroughly, accurately, and completely.<\/li>\n<li>Staff will reach out once the form is submitted to confirm a time\/date.<\/li>\n<li>Arrive at the appointment with the samples prepared as described in the\u00a0<a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/education\/sorting-information\/\">Flow Facility Sorting SOP<\/a>.<\/li>\n<li>Staff will upload data to OneDrive and send you a link to the folder via email. This link will also be available via the iLabs request once the request is completed.<\/li>\n<\/ol>\n<p>If you have any questions regarding the Sorting Request Form or the best way to design your sorting experiment please reach out to the <a href=\"mailto:UNCFlowCyto@med.unc.edu\">UNC Core Staff<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Expectations for Staff-Assisted Workflow: Staff check submissions daily, however, availability depends on instrument AND staff time. Notice of 1 week is preferred; however, times\/dates are not guaranteed. Staff do their best to meet your preferred time and date. Responses are expected within 48 hours (about 2 days). Instruments are used by independent users and staff, &hellip; <a href=\"https:\/\/www.med.unc.edu\/flowcytometry\/services\/staff-assisted\/\" aria-label=\"Read more about Staff-Assisted Acquisition &amp; Sorting\">Read more<\/a><\/p>\n","protected":false},"author":6662,"featured_media":0,"parent":2261,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-3672","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/3672","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/users\/6662"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/comments?post=3672"}],"version-history":[{"count":15,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/3672\/revisions"}],"predecessor-version":[{"id":4565,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/3672\/revisions\/4565"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/pages\/2261"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/flowcytometry\/wp-json\/wp\/v2\/media?parent=3672"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}