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High-Throughput Sequencing Facility (HTSF)
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External Account Sample Submission Form
External Account Sample Submission Form
External Account Sample Submission Form
Step
1
of
5
20%
External users of the HTSF
Customer service group requires 2 documents to be filled out and returned (Contact HTSF customer service with any questions).
External Account Sample Submission form
TracSeq Submission Manifest
A Customer Service Rep will submit for you and return the following:
Confirmed manifest (a copy is required to be included in shipping container with samples)
Confirmed shipment date
Instructions for shipping
Custom Primer (if applicable) Preparation Instructions
Please note: samples CAN NOT be shipped until HTSF submission has been completed
1. Administrative Information
Date of Submission
*
MM slash DD slash YYYY
HTSF Account Name
*
Submitter Name
*
Submitter Email
*
Submitter Phone
*
PI Name
*
PI Email
*
PI Phone
Additional Project Staff Name
Additional Project Staff Email
Additional Project Staff Phone
Billing
Accountant Name
*
Accountant Email
*
Organization
*
Department
*
Billing Address
Street Address
Address Line 2
City
State / Province / Region
ZIP / Postal Code
Afghanistan
Albania
Algeria
American Samoa
Andorra
Angola
Anguilla
Antarctica
Antigua and Barbuda
Argentina
Armenia
Aruba
Australia
Austria
Azerbaijan
Bahamas
Bahrain
Bangladesh
Barbados
Belarus
Belgium
Belize
Benin
Bermuda
Bhutan
Bolivia
Bonaire, Sint Eustatius and Saba
Bosnia and Herzegovina
Botswana
Bouvet Island
Brazil
British Indian Ocean Territory
Brunei Darussalam
Bulgaria
Burkina Faso
Burundi
Cabo Verde
Cambodia
Cameroon
Canada
Cayman Islands
Central African Republic
Chad
Chile
China
Christmas Island
Cocos Islands
Colombia
Comoros
Congo
Congo, Democratic Republic of the
Cook Islands
Costa Rica
Croatia
Cuba
Curaçao
Cyprus
Czechia
Côte d'Ivoire
Denmark
Djibouti
Dominica
Dominican Republic
Ecuador
Egypt
El Salvador
Equatorial Guinea
Eritrea
Estonia
Eswatini
Ethiopia
Falkland Islands
Faroe Islands
Fiji
Finland
France
French Guiana
French Polynesia
French Southern Territories
Gabon
Gambia
Georgia
Germany
Ghana
Gibraltar
Greece
Greenland
Grenada
Guadeloupe
Guam
Guatemala
Guernsey
Guinea
Guinea-Bissau
Guyana
Haiti
Heard Island and McDonald Islands
Holy See
Honduras
Hong Kong
Hungary
Iceland
India
Indonesia
Iran
Iraq
Ireland
Isle of Man
Israel
Italy
Jamaica
Japan
Jersey
Jordan
Kazakhstan
Kenya
Kiribati
Korea, Democratic People's Republic of
Korea, Republic of
Kuwait
Kyrgyzstan
Lao People's Democratic Republic
Latvia
Lebanon
Lesotho
Liberia
Libya
Liechtenstein
Lithuania
Luxembourg
Macao
Madagascar
Malawi
Malaysia
Maldives
Mali
Malta
Marshall Islands
Martinique
Mauritania
Mauritius
Mayotte
Mexico
Micronesia
Moldova
Monaco
Mongolia
Montenegro
Montserrat
Morocco
Mozambique
Myanmar
Namibia
Nauru
Nepal
Netherlands
New Caledonia
New Zealand
Nicaragua
Niger
Nigeria
Niue
Norfolk Island
North Macedonia
Northern Mariana Islands
Norway
Oman
Pakistan
Palau
Palestine, State of
Panama
Papua New Guinea
Paraguay
Peru
Philippines
Pitcairn
Poland
Portugal
Puerto Rico
Qatar
Romania
Russian Federation
Rwanda
Réunion
Saint Barthélemy
Saint Helena, Ascension and Tristan da Cunha
Saint Kitts and Nevis
Saint Lucia
Saint Martin
Saint Pierre and Miquelon
Saint Vincent and the Grenadines
Samoa
San Marino
Sao Tome and Principe
Saudi Arabia
Senegal
Serbia
Seychelles
Sierra Leone
Singapore
Sint Maarten
Slovakia
Slovenia
Solomon Islands
Somalia
South Africa
South Georgia and the South Sandwich Islands
South Sudan
Spain
Sri Lanka
Sudan
Suriname
Svalbard and Jan Mayen
Sweden
Switzerland
Syria Arab Republic
Taiwan
Tajikistan
Tanzania, the United Republic of
Thailand
Timor-Leste
Togo
Tokelau
Tonga
Trinidad and Tobago
Tunisia
Turkmenistan
Turks and Caicos Islands
Tuvalu
Türkiye
US Minor Outlying Islands
Uganda
Ukraine
United Arab Emirates
United Kingdom
United States
Uruguay
Uzbekistan
Vanuatu
Venezuela
Viet Nam
Virgin Islands, British
Virgin Islands, U.S.
Wallis and Futuna
Western Sahara
Yemen
Zambia
Zimbabwe
Åland Islands
Country
Please indicate one of the following for payment for services:
PO#
*attach PO copy below
PO Copy
Max. file size: 98 MB.
Check #
Date Check Was Sent
MM slash DD slash YYYY
Shipping
Preferred Shipment Date
*
MM slash DD slash YYYY
HTSF will confirm available dates
Preferred Data Delivery Deadline (HTSF will confirm)
*
Study Shipping Site Address
*
Street Address
Address Line 2
City
State / Province / Region
ZIP / Postal Code
Afghanistan
Albania
Algeria
American Samoa
Andorra
Angola
Anguilla
Antarctica
Antigua and Barbuda
Argentina
Armenia
Aruba
Australia
Austria
Azerbaijan
Bahamas
Bahrain
Bangladesh
Barbados
Belarus
Belgium
Belize
Benin
Bermuda
Bhutan
Bolivia
Bonaire, Sint Eustatius and Saba
Bosnia and Herzegovina
Botswana
Bouvet Island
Brazil
British Indian Ocean Territory
Brunei Darussalam
Bulgaria
Burkina Faso
Burundi
Cabo Verde
Cambodia
Cameroon
Canada
Cayman Islands
Central African Republic
Chad
Chile
China
Christmas Island
Cocos Islands
Colombia
Comoros
Congo
Congo, Democratic Republic of the
Cook Islands
Costa Rica
Croatia
Cuba
Curaçao
Cyprus
Czechia
Côte d'Ivoire
Denmark
Djibouti
Dominica
Dominican Republic
Ecuador
Egypt
El Salvador
Equatorial Guinea
Eritrea
Estonia
Eswatini
Ethiopia
Falkland Islands
Faroe Islands
Fiji
Finland
France
French Guiana
French Polynesia
French Southern Territories
Gabon
Gambia
Georgia
Germany
Ghana
Gibraltar
Greece
Greenland
Grenada
Guadeloupe
Guam
Guatemala
Guernsey
Guinea
Guinea-Bissau
Guyana
Haiti
Heard Island and McDonald Islands
Holy See
Honduras
Hong Kong
Hungary
Iceland
India
Indonesia
Iran
Iraq
Ireland
Isle of Man
Israel
Italy
Jamaica
Japan
Jersey
Jordan
Kazakhstan
Kenya
Kiribati
Korea, Democratic People's Republic of
Korea, Republic of
Kuwait
Kyrgyzstan
Lao People's Democratic Republic
Latvia
Lebanon
Lesotho
Liberia
Libya
Liechtenstein
Lithuania
Luxembourg
Macao
Madagascar
Malawi
Malaysia
Maldives
Mali
Malta
Marshall Islands
Martinique
Mauritania
Mauritius
Mayotte
Mexico
Micronesia
Moldova
Monaco
Mongolia
Montenegro
Montserrat
Morocco
Mozambique
Myanmar
Namibia
Nauru
Nepal
Netherlands
New Caledonia
New Zealand
Nicaragua
Niger
Nigeria
Niue
Norfolk Island
North Macedonia
Northern Mariana Islands
Norway
Oman
Pakistan
Palau
Palestine, State of
Panama
Papua New Guinea
Paraguay
Peru
Philippines
Pitcairn
Poland
Portugal
Puerto Rico
Qatar
Romania
Russian Federation
Rwanda
Réunion
Saint Barthélemy
Saint Helena, Ascension and Tristan da Cunha
Saint Kitts and Nevis
Saint Lucia
Saint Martin
Saint Pierre and Miquelon
Saint Vincent and the Grenadines
Samoa
San Marino
Sao Tome and Principe
Saudi Arabia
Senegal
Serbia
Seychelles
Sierra Leone
Singapore
Sint Maarten
Slovakia
Slovenia
Solomon Islands
Somalia
South Africa
South Georgia and the South Sandwich Islands
South Sudan
Spain
Sri Lanka
Sudan
Suriname
Svalbard and Jan Mayen
Sweden
Switzerland
Syria Arab Republic
Taiwan
Tajikistan
Tanzania, the United Republic of
Thailand
Timor-Leste
Togo
Tokelau
Tonga
Trinidad and Tobago
Tunisia
Turkmenistan
Turks and Caicos Islands
Tuvalu
Türkiye
US Minor Outlying Islands
Uganda
Ukraine
United Arab Emirates
United Kingdom
United States
Uruguay
Uzbekistan
Vanuatu
Venezuela
Viet Nam
Virgin Islands, British
Virgin Islands, U.S.
Wallis and Futuna
Western Sahara
Yemen
Zambia
Zimbabwe
Åland Islands
Country
# of Samples to be Shipped
*
Method of Shipment
*
Ambient Temp
Wet Ice (4oC)
Dry Ice
Liquid N2 (Cryoport)
2. Source and Material Submitted
Material Submitting
*
Sample (Raw Material)
Study Made Libraries
Study Made Pools
Source Material
*
list starting RNA, DNA, Amplicons, Exosome, etc.
If RNA is from tissue, indicate preservation method
Fresh Frozen
Formalin Fixed Paraffin Embedded
Library Prep Method
*
If HTSF is to make library- list type to be made
If Study Made library, list method used
If Custom, Send protocol used or paper referenced for preparation and please describe as best you can
3. Pooling
Will HTSF Pool Samples?
*
Yes
No
If yes, How many samples/pool?
HTSF will pool by #libraries/pool
Customer will indicate which samples to pool together
Barcode Type
*
Not Specified
Single (1D)
Dual (2D)
select "Not Specified" if HTSF is making libraries. HTSF will choose best application for your library type, sample # and seq set up
4. Sequencing
Sequencing
*
Chose Sequencing Platform (below)
No Sequencing
Sequencing to be determined
Sequencing Platform
available platforms listed below
Read Type
Single End
Paired End
Sequencing Cycles
Standard (indicate below)
Custom (indicate below)
Standard Cycles
ie: 50x, 75x, 150x, 250x, 300x (not all cycles are available on all platforms)
Custom Cycle Length
(only to be filled out is custom cycles are required)
Read = cycles for library fragment
Index = cycles for barcode (cycles will = barcode length)
Read 1
Index 1
Index 2 (req. for DUAL barcodes)
Read 2 (req. for PE reads)
Additional PhiX Required?
Yes
No
Unsure. HTSF will determine need based on library type
(Per Illumina, 1% PhiX is always added. You will not receive PhiX reads in your data)
If yes, what % should be added to the run?
Custom Primer required?
No
Old Nextera (HTSF keeps a stock)
Yes (please indicate custom primer info below)
Custom Primer Information
( Custom primer will need to be supplied by study, directions will be included with HTSF confirmation email)
Custom Primer #1 Name
Custom Primer #1 Primer nt sequence
Custom Primer #2 Name
(only if 2 primers are to be used )
Custom Primer #2 Primer nt sequence
(only if 2 primers are to be used )
Available Platforms
Miseq
Novaseq -SP
Miseq Nano
Novaseq-S1
HiSeq 2500 Rapid Run
Novaseq-S2
HiSeq 2500-v4 High Output
Novaseq-S4
Hiseq 4000
*XP Kits available for all NOVASEQ options (allows single lane per pool to be run)
5. Container and Diluent
Container Type
*
1.5ml - 1.7ml snap cap or screw cap tube
Matrix tube
BioRad 96 well Skirted PCR plate, hard-shelled
Slide
Diluent
*
dH20
Elution Buffer (EB) : defined as Tris-Cl, pH ~8 (no EDTA)
NO diluent ( Tissue on slide or Lyophilized Pellet)
6. Bioinformatics
Data Delivery Method
*
Delivery via URL
Data Delivery Contact Name
*
Data Delivery Contact Email
*
Reference Genome
*
Do you need data analysis help?
*
Yes
No
Batch Special Needs
• Note this in general instructions for the entire submission you want the HTSF to be aware of (100 character limit). This is not where individual samples issues are listed. That will be noted on the manifest.
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