{"id":2650,"date":"2020-05-20T10:44:28","date_gmt":"2020-05-20T14:44:28","guid":{"rendered":"https:\/\/www.med.unc.edu\/genomics\/?page_id=2650"},"modified":"2023-01-20T19:16:16","modified_gmt":"2023-01-21T00:16:16","slug":"overview-of-services","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/genomics\/overview-of-services\/","title":{"rendered":"Overview of Services"},"content":{"rendered":"<p>The HTSF is a full-service sequencing facility to assist with your genetic and genomic research. We offer a wide number of library preparations (Illumina kits, non Illumina kits, novel R&amp;D, Oxford Nanopore, BioNano). There are multiple sequencing platforms (MiSeq, NextSeq 2000, NovaSeq 6000, BioNano, Oxford Nanopore). Our services also include using state-of-the-art techniques and data analyses (via our sister core BARC).<\/p>\n<p>Please contact us if you are interested in applications not listed. For input requirements for library preparation methods, please go to the <a href=\"https:\/\/www.med.unc.edu\/genomics\/submissions\/\">Submissions page<\/a>\u00a0for more information.<\/p>\n<script>\n\n    (function($) {\n        $(\"body, html\").addClass(\"heels_full_width_overflow\");\n    })(jQuery);\n\n   <\/script>\n   <div style=\"background-color:#007FAE;\" class=\"full-width-contained whitebackground \">\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" src=\"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/building_louvers_1-1.jpg\" alt=\"Genome Sciences Building\" width=\"923\" height=\"672\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<h1 class=\"header-landing-white\"><a style=\"color: #fff\" href=\"https:\/\/www.med.unc.edu\/genomics\/overview-of-services\/library-perparation\/\">Library Preparation<\/a><\/h1>\n<div style=\"color: #fff\">\n<p>The HTSF offers multiple library preparation services that include:<\/p>\n<ul>\n<li>Illumina based technology libraries \u2013 manual and robotics production<\/li>\n<li>Non-Illumina based library preparation<\/li>\n<li>Custom libraries<\/li>\n<li>R&amp;D novel library preps \u2013 with consultation with HTSF<\/li>\n<li>Oxford Nanopore and Bionano Libraries<\/li>\n<\/ul>\n<p>Do not see a library you are thinking about? Have an idea but not sure how to go about it? Come speak with HTSF about your needs, we can make suggestions as to the best options to fit your budget and data needs.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<script>\n\n    (function($) {\n        $(\"body, html\").addClass(\"heels_full_width_overflow\");\n    })(jQuery);\n\n   <\/script>\n   <div style=\"background-color:#fff;\" class=\"full-width-contained whitebackground \">\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/thumbnail_Building_clouds_1-1.jpg\" alt=\"Genome Sciences Building\" width=\"200\" height=\"720\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<h1 class=\"header-landing-blue\"><a href=\"https:\/\/www.med.unc.edu\/genomics\/overview-of-services\/dna-sequencing\/\">DNA Library Preparation and Sequencing<\/a><\/h1>\n<div>DNA sequencing can be applied to small, targeted regions or the entire genome through a variety of methods, enabling researchers to investigate and better understand health and disease. The HTSF offers various methods of DNA library preparation and sequencing which include Complete Genome Re-Sequencing, Low-Input Double Stranded DNA Products, Metagenomic sequencing, custom library preparation and sequencing, and Reduced Representation Bisulfite Sequencing.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<script>\n\n    (function($) {\n        $(\"body, html\").addClass(\"heels_full_width_overflow\");\n    })(jQuery);\n\n   <\/script>\n   <div style=\"background-color:#007FAE;\" class=\"full-width-contained whitebackground \">\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" src=\"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/Building_courtyard_1.jpg\" alt=\"Genome Sciences Building\" width=\"810\" height=\"690\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<h1 class=\"header-landing-white\"><a style=\"color: #fff\" href=\"https:\/\/www.med.unc.edu\/genomics\/overview-of-services\/rna-sequencing\/\">RNA Library Preparation and Sequencing<\/a><\/h1>\n<div style=\"color: #fff\">RNA Sequencing with next-generation sequencing (NGS) is frequently the method of choice for researchers studying the transcriptome. The HTSF currently offers various sRNA library preparation and sequencing services which include Total RNA-Seq, mRNA-Seq, and small RNA sequencing.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<script>\n\n    (function($) {\n        $(\"body, html\").addClass(\"heels_full_width_overflow\");\n    })(jQuery);\n\n   <\/script>\n   <div style=\"background-color:#fff;\" class=\"full-width-contained whitebackground \">\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" src=\"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/Building_belltower-drive_1.jpg\" alt=\"Genome Sciences Building\" width=\"977\" height=\"629\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<h1 class=\"header-landing-blue\"><a href=\"https:\/\/www.med.unc.edu\/genomics\/overview-of-services\/non-illumina-services\/\">Alternative Library Services<\/a><\/h1>\n<div>Currently, the HTSF does not offer non-Illumina library preparation services for both 10X Genomics and HTG EdgeSeq. The HTSF does offer sequencing for these systems. Please review information for these service on this tab. HTSF suggests Cores which can prepare these libraries for you.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<script>\n\n    (function($) {\n        $(\"body, html\").addClass(\"heels_full_width_overflow\");\n    })(jQuery);\n\n   <\/script>\n   <div style=\"background-color:#007FAE;\" class=\"full-width-contained whitebackground \">\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" src=\"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/Building_tree_1.jpg\" alt=\"Genome Sciences Building\" width=\"702\" height=\"570\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<h1 class=\"header-landing-white\"><a style=\"color: #fff\" href=\"https:\/\/www.med.unc.edu\/genomics\/overview-of-services\/long-read-sequencing\/\">Long Read Sequencing<\/a><\/h1>\n<div style=\"color: #fff\">Long-read sequencing offers numerous advantages over short-read sequencing via the NovaSeq, HiSeq, NextSeq, and MiSeq. Short read sequencing produces reads of up to 600 bases while long-read sequencing technologies routinely generate reads over 10 kb. Long reads sequencing presents numerous benefits for both de novo and alignment-based genome assembly. Long reads can cover the whole structure variant in one read which results in more accurate genome assemblies and a better understanding of genome architecture in genomic diseases.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<script>\n\n    (function($) {\n        $(\"body, html\").addClass(\"heels_full_width_overflow\");\n    })(jQuery);\n\n   <\/script>\n   <div style=\"background-color:#fff;\" class=\"full-width-contained whitebackground \">\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" src=\"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/Building_walkway_1.jpg\" alt=\"Genome Sciences Building\" width=\"1078\" height=\"649\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-6 oscitas-bootstrap-container\">\n<h1 class=\"header-landing-blue\"><a href=\"https:\/\/www.med.unc.edu\/genomics\/overview-of-services\/bioinformatics\/\">Bioinformatics<\/a><\/h1>\n<p>&nbsp;<\/p>\n<div>Bioinformatics forms an integral part of high throughput sequencing workflow. Bioinformatics and Analytics Research Collaborative (BARC) is a new research support group in UNC-School of Medicine. BARC now includes personnel who provide critical bioinformatics support for UNC-HTSF.<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The HTSF is a full-service sequencing facility to assist with your genetic and genomic research. We offer a wide number of library preparations (Illumina kits, non Illumina kits, novel R&amp;D, Oxford Nanopore, BioNano). There are multiple sequencing platforms (MiSeq, NextSeq 2000, NovaSeq 6000, BioNano, Oxford Nanopore). Our services also include using state-of-the-art techniques and data &hellip; <a href=\"https:\/\/www.med.unc.edu\/genomics\/overview-of-services\/\" aria-label=\"Read more about Overview of Services\">Read more<\/a><\/p>\n","protected":false},"author":64010,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2650","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Overview of Services - High-Throughput Sequencing Facility (HTSF)<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/genomics\/overview-of-services\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Overview of Services - High-Throughput Sequencing Facility (HTSF)\" \/>\n<meta property=\"og:description\" content=\"The HTSF is a full-service sequencing facility to assist with your genetic and genomic research. 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