{"id":3365,"date":"2020-07-01T15:20:44","date_gmt":"2020-07-01T19:20:44","guid":{"rendered":"https:\/\/www.med.unc.edu\/genomics\/?p=3365"},"modified":"2020-08-24T10:50:48","modified_gmt":"2020-08-24T14:50:48","slug":"novaseq-6000-vs-highseq-2500-evaluation-by-mrna-seq","status":"publish","type":"post","link":"https:\/\/www.med.unc.edu\/genomics\/novaseq-6000-vs-highseq-2500-evaluation-by-mrna-seq\/","title":{"rendered":"NovaSeq 6000 vs HighSeq 2500, Evaluation by mRNA-seq"},"content":{"rendered":"

<\/p>\n

This is a presentation on the comparison of the same series of libraries run on both the HS2500 and the Novaseq. These were TruSeq stranded mRNA. Pools of 4 libraries were run on the HiSeq 2500\/ paired end\/ 50x. The same pools were then pooled into larger pools (pools of pools) to be run on the NovaseqS1\/ PE\/50x to achieve similar data coverage. This presentation covered the differences in the platforms, the data yielded from each and lessons learned.<\/p>\nDownload PDF<\/a>\n","protected":false},"excerpt":{"rendered":"

This is a presentation on the comparison of the same series of libraries run on both the HS2500 and the Novaseq. These were TruSeq stranded mRNA. Pools of 4 libraries were run on the HiSeq 2500\/ paired end\/ 50x. The same pools were then pooled into larger pools (pools of pools) to be run on … Read more<\/a><\/p>\n","protected":false},"author":64010,"featured_media":3366,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[11],"tags":[],"class_list":["post-3365","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-whats-new","odd"],"acf":[],"featured_image":"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/Screen-Shot-2020-06-22-at-9.21.13-AM.png","featured_image_medium":"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/Screen-Shot-2020-06-22-at-9.21.13-AM-300x225.png","featured_image_medium_large":"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/Screen-Shot-2020-06-22-at-9.21.13-AM-768x576.png","featured_image_large":"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/Screen-Shot-2020-06-22-at-9.21.13-AM-1024x768.png","featured_image_thumbnail":"https:\/\/www.med.unc.edu\/genomics\/wp-content\/uploads\/sites\/708\/2020\/07\/Screen-Shot-2020-06-22-at-9.21.13-AM-150x150.png","featured_image_alt":"","category_details":[{"name":"What's New","link":"https:\/\/www.med.unc.edu\/genomics\/category\/whats-new\/"}],"tag_details":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/genomics\/wp-json\/wp\/v2\/posts\/3365","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/genomics\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.med.unc.edu\/genomics\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/genomics\/wp-json\/wp\/v2\/users\/64010"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/genomics\/wp-json\/wp\/v2\/comments?post=3365"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/genomics\/wp-json\/wp\/v2\/posts\/3365\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/genomics\/wp-json\/wp\/v2\/media\/3366"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/genomics\/wp-json\/wp\/v2\/media?parent=3365"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.med.unc.edu\/genomics\/wp-json\/wp\/v2\/categories?post=3365"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.med.unc.edu\/genomics\/wp-json\/wp\/v2\/tags?post=3365"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}