2025 Tissue Clearing & Imaging Conference
2025-04-07 08:00 - 17:00, 2025-04-07 08:45 - 2025-04-08 15:30 (America/New_York) | Kirkland Auditorium, Koury Oral Health Sciences Building, 120 Dental Cir, Chapel Hill, NC 27514, 120 Dental Cir, Chapel Hill, NC, 27599, United States
This conference promises to be an exciting platform for learning and networking, featuring a keynote presentation by Dr. G. Allan Johnson from Duke University. The event will bring together leading researchers and professionals in the field of tissue clearing and imaging, offering insights into the latest advancements and techniques.

• Date: Monday, April 7th, and Tuesday, April 8th
• Location: Kirkland Auditorium, Koury Oral Health Sciences Building, 120 Dental Cir, Chapel Hill, NC 27514
• Keynote Speaker: Dr. G. Allan Johnson, Duke University

Free Registration: https://tinyurl.com/3hkbmf5j
Conference Talk Schedule: https://tinyurl.com/a7t3rjr4

We look forward to your participation and engagement at this event. If you have any questions or need further information, please do not hesitate to contact us.

Ian Curtin
Ph.D Candidate in Genetics and Molecular Biology Cirriculum
Stein Lab | University of North Carolina at Chapel Hill
iancurtin@unc.edu

Laser Microdissection Sample Prep
2025-09-17 14:00 - 15:00
Leica LMD Sample Prep Talk (Virtual) – Wednesday Sept. 17th 2-3pm
email Kurt Doyen at Leica Microsystems for login information

Webinar: Single Molecule Localization Microscopy and TIRF
2025-10-01 08:00 - 17:00
We are pleased to invite you to an online seminar by Abbelight, held in preparation for the Single Molecule Localization Microscopy and TIRF Microscopy workshop, to explore the latest advancements and diverse applications of SMLM and TIRF microscopy, and to discuss how to prepare the perfect sample for imaging down to 20 nm resolution.

The seminar will cover the following topics:

Breaking Barriers in Bioimaging: Abbelight’s Cutting-Edge Technologies
Discover what Abbelight has to offer for your research.
Introduction to SMLM: From Concepts to Data Analysis
A deep dive into the latest nanoscopy innovations and their applications in fields such as cell biology, microbiology, neuroscience, extracellular vesicles, and more.
Sample Preparation for SMLM: Tips and Tricks
Learn how to prepare your samples and achieve imaging down to 20 nm resolution

Laser Microdissection on-site Demo - Leica LMD 7
2025-10-06 08:00 - 2025-10-09 17:00 | HIC@UNC, 229 Taylor Hall, 109 Mason Farm Rd., Chapel Hill, NC, 27599, United States
Hooker Imaging Core, 229 Taylor Hall
Precisely remove portions of your sample through the microscope for downstream analysis. Demo samples available to demonstrate general system function. We also want to see your samples!

Detailed sample prep guide (pdf)
9/19/2025 Webinar describing system capabilities, applications, workflows, and a broad overview of sample preparation

PPT slides (pdf)

Please contact both Kurt Doyen (Sr. Account Manager for UNC) and Natasha Fernandez Dias Granados (Leica’s spatial biology Applications Specialist). if you are interested in seeing the system in action. They will want to discuss your project prior to the on-site demo to maximize your likelihood for success while the system is on site.
To use your own sample during the on-site demo, some advanced planning is optimal:

You must mount your samples on specialized sample holders with a specific membrane. Leica can provide appropriate sample holders for the demo.
Different kinds of membranes are required for different downstream applications (RNA, mass spec, PCR, etc.)

Resources:

Online sample preparation guides

Workflows & Protocols Plant Laser Microdissection
Protocol LMD of cryo-sectioned Brain Tissue for Proteomics
Protocol LMD of FFPE Brain Tissue for Proteomics

9/19/2025 Webinar describing system capabilities, applications, workflows, and a broad overview of sample preparation

PPT slides (pdf)

Leica LMD7 brochure (pdf)
Lots of documents related to LMD operation and applications

Workshop--Single Molecule Localization Microscopy and TIRF
2025-11-03 08:00 - 2025-11-07 17:00
Test out the Abbelight SMLM and TIRF system
Taylor Hall 229
Co-hosted by Jenn Marks with Evident Scientific (formerly Olympus).
See the system working with one of the demo slides or test your own sample.
Sign up for a time slot with the 👉 Doodle Sign-Up Sheet
For maximal success with your samples, contact Paul Barthélémy as soon as possible to ensure you have the optimal sample holders and fluorophores (pdf). We have a small amount of secondary antibodies with appropriate fluorophores available on-site for sample preparation prior to your imaging session.
Watch the UNC Sample Prep Webinar from Oct. 9, 2025.
Practical notes:
1: SMLM will not work with your standard confocal sample of a coverslip mounted on a slide with mounting media using whichever fluorophores you have in your freezer. The preparation is very similar to your confocal protocol, but there are a few extremely critical details that might differ. You will likely need to generate a specific sample for the demo.
2: Fluorophores needed for SMLM also work great for standard fluorescence applications, so anything you might need to buy for a successful demo can likely be used for other applications.
PDF product brochure

Spatial Biology & Single-Cell Seminar presented by Bruker
2025-11-12 10:00 - 15:00
10am-3pm MBRB
Details and free registration
https://go.brukerspatialbiology.com/UNC-SingleCellSymposium-REG.html

QuPath working group - Intro organizational meeting
2026-02-20 12:00 - 13:00
In person: MEJ 3102 — feel free to bring your lunch
Online: Zoom link
Come learn about what QuPath can do and help us structure on-campus resources for learning how to use this powerful image analysis software too.

Introductions – Wendy/Shannon
What is QuPath? (very brief); why is it different than other open-source (or UNC paid for softwares)
Why are we here? Why imaging/analysis folks think this is important
Anticipated activities throughout the semester from our POV

Workshop/demo (1 hr), Co-working time – talk to each other to solve issues, “Data club” type: presentations of data/analysis/problems

How to get help:

Teams group for “help” calls – or something similar; what is best way to contact everyone, forum.Image.sc, Contact information for us

How can we support you? Brainstorming session

Introduction to QuPath for Whole Slide Image Analysis
2026-03-10 08:00 - 17:00
QuPath from an Experts Perspective
Sara McArdle, PhD
Sr. Staff Scientist, Microscopy Core, La Jolla Institute for Immunology
Virtual seminar
Live group viewing MEJ 3106
Online https://go.unc.edu/Jg25X

QuPath working group
2026-03-10 08:00 - 17:00
MEJ 3102
Specific topic TBD
