Microscopy Café: Analysis Afternoons
2024-05-14 14:30 - 16:00 (America/New_York) | 3106 Mary Ellen Jones

Microscopy Café: Theory Thursdays
2024-05-23 10:30 - 11:30 | 6004 Marsico

Microscopy Cafe: Analysis Afternoons
2024-05-28 14:30 - 16:00, 2024-06-11 14:30 - 16:00, 2024-06-25 14:20 - 16:00 | 3106 Mary Ellen Jones

Microscopy Cafe: Theory Thursdays
2024-06-20 10:30 - 11:30, 2024-07-25 10:30 - 11:30 | 6004 Marsico

Demo: Nikon AXR with NSPARC
2025-01-13 - 2025-01-17 | HIC@UNC, 229 Taylor Hall, 109 Mason Farm Rd., Chapel Hill, NC, 27599, United States
Nikon is bringing their latest flagship confocal AXR with NSPARK detector to UNC. Resonant scanning, high efficiency detectors and super-resolution to ~120nm. Email Kelley Lee to book a slot.

POSTPONED Workshop: Maximizing Spectral Flexibility with the Leica Stellaris confocal
2025-01-28 - 2025-01-29 | HIC@UNC, 229 Taylor Hall, 109 Mason Farm Rd., Chapel Hill, NC, 27599, United States
POSTPONED to late March (updated 1/27/25)

Join Wendy Salmon (HIC) and experts from Leica to learn how to optimize wavelength selections, separate fluorophore signal from autofluorescence, and image 7-15 fluorophores in the same sample. Leica will have a sample of beads with 15 different spectra and some standard tissue slide. Please let us know if you’d like sample preparation information for your own samples! This will be a combination of seminars, demonstrations on the Leica Stellaris 8 in the HIC, and appointments with Leica experts for one-on-one assistance with image collection or data handling. Links for online signup coming soon (1/7/2025).

Tomocube webinar 2025
2025-02-03 14:00 - 15:00
Join this webinar to learn about the Tomocube label-free holotomography system that provides fast, gentle, high-resolution 3D imaging based on differences in the refractive index of different cell compartments. It is also compatible with fluorescence. It’s a great system for live cell imaging.
Zoom webinar link
Flier for Tomocube UNC Webinar Feb. 3, 2025
Brochure TCG-HTX-06 Tomocube HT-X1 Series Brochure (Web, LowRes)

Workshop: Confocal basics and advanced acquisition with Zeiss LSM 980 and Zen Blue.
2025-02-05 - 2025-02-07
Take your confocal imaging to the next level with this 3-part workshop. It helps if you complete the free online registration!
All online sessions will be in this Zoom room.
Schedule:

Wed., Feb. 5. Session 1: Optimizing Confocal Imaging Efficiency

1-1:45pm Seminar, Marsico 2004 and online

2-4pm Demonstration, Taylor Hall 229 and online

Thur., Feb 6, Session 2: Improving Throughput via Tiles and Positions

1-1:45pm Seminar, Marsico 2004 and online

2-4pm Demonstration, Taylor Hall 229 and online

Fri., Feb 7, Session 3: Balancing Resolution and Imaging Speed with Airyscan

1-1:45pm Seminar, Mary Ellen Jones 3112 and online (NOTE different location)

2-4pm Demonstration, Taylor Hall 229 and online

Demo: Zeiss LSM 980 with spectral imaging, Airyscan2 and NIR
2025-02-07 08:00 - 2025-02-13 17:00 | HIC@UNC, 229 Taylor Hall, 109 Mason Farm Rd., Chapel Hill, NC, 27599, United States
If you would like a one-on-one session on the Zeiss 980, please complete Zeiss’s quick sample survey and Garrick Koermer from Zeiss will be in touch to get you on the calendar.
Sarah Cohen and Wendy Salmon (HIC) are putting together an S10 shared instrumentation grant to upgrade our Zeiss LSM 880 Spectral Imaging system. We need to test how the live spectral imaging and Airyscan2 compare to our current system. If you currently use, or are thinking of using, either of those tools, please let Wendy know ASAP so we can prepare for a successful demo. Also reach out if you want some one-on-one assistance from the Zeiss experts regardless of your participation in the grant. Instrument has incubation enclosure for live imaging experiments.

Tomocube on-site Demo
2025-02-24 - 2025-02-28
Come try out the Tomocube label-free holotomography system that provides fast, gentle, high-resolution 3D imaging based on differences in the refractive index of different cell compartments. It is also compatible with fluorescence. It’s a great system for live cell imaging.
Hosted by Dr. Nat Prunet in the Biology Department Microscopy Core. Please contact Nat to schedule time on the system.
TCG-HTX-06 Tomocube HT-X1 Series Brochure (Web, LowRes)

2025 Tissue Clearing & Imaging Conference
2025-04-07 08:00 - 17:00, 2025-04-07 08:45 - 2025-04-08 15:30 | Kirkland Auditorium, Koury Oral Health Sciences Building, 120 Dental Cir, Chapel Hill, NC 27514, 120 Dental Cir, Chapel Hill, NC, 27599, United States
This conference promises to be an exciting platform for learning and networking, featuring a keynote presentation by Dr. G. Allan Johnson from Duke University. The event will bring together leading researchers and professionals in the field of tissue clearing and imaging, offering insights into the latest advancements and techniques.

• Date: Monday, April 7th, and Tuesday, April 8th
• Location: Kirkland Auditorium, Koury Oral Health Sciences Building, 120 Dental Cir, Chapel Hill, NC 27514
• Keynote Speaker: Dr. G. Allan Johnson, Duke University

Free Registration: https://tinyurl.com/3hkbmf5j
Conference Talk Schedule: https://tinyurl.com/a7t3rjr4

We look forward to your participation and engagement at this event. If you have any questions or need further information, please do not hesitate to contact us.

Ian Curtin
Ph.D Candidate in Genetics and Molecular Biology Cirriculum
Stein Lab | University of North Carolina at Chapel Hill
iancurtin@unc.edu

Laser Microdissection Sample Prep
2025-09-17 14:00 - 15:00
Leica LMD Sample Prep Talk (Virtual) – Wednesday Sept. 17th 2-3pm
email Kurt Doyen at Leica Microsystems for login information

Webinar: Single Molecule Localization Microscopy and TIRF
2025-10-01 08:00 - 17:00
We are pleased to invite you to an online seminar by Abbelight, held in preparation for the Single Molecule Localization Microscopy and TIRF Microscopy workshop, to explore the latest advancements and diverse applications of SMLM and TIRF microscopy, and to discuss how to prepare the perfect sample for imaging down to 20 nm resolution.

The seminar will cover the following topics:

Breaking Barriers in Bioimaging: Abbelight’s Cutting-Edge Technologies
Discover what Abbelight has to offer for your research.
Introduction to SMLM: From Concepts to Data Analysis
A deep dive into the latest nanoscopy innovations and their applications in fields such as cell biology, microbiology, neuroscience, extracellular vesicles, and more.
Sample Preparation for SMLM: Tips and Tricks
Learn how to prepare your samples and achieve imaging down to 20 nm resolution

Laser Microdissection on-site Demo - Leica LMD 7
2025-10-06 08:00 - 2025-10-09 17:00 | HIC@UNC, 229 Taylor Hall, 109 Mason Farm Rd., Chapel Hill, NC, 27599, United States
Hooker Imaging Core, 229 Taylor Hall
Precisely remove portions of your sample through the microscope for downstream analysis. Demo samples available to demonstrate general system function. We also want to see your samples!

Detailed sample prep guide (pdf)
9/19/2025 Webinar describing system capabilities, applications, workflows, and a broad overview of sample preparation

PPT slides (pdf)

Please contact both Kurt Doyen (Sr. Account Manager for UNC) and Natasha Fernandez Dias Granados (Leica’s spatial biology Applications Specialist). if you are interested in seeing the system in action. They will want to discuss your project prior to the on-site demo to maximize your likelihood for success while the system is on site.
To use your own sample during the on-site demo, some advanced planning is optimal:

You must mount your samples on specialized sample holders with a specific membrane. Leica can provide appropriate sample holders for the demo.
Different kinds of membranes are required for different downstream applications (RNA, mass spec, PCR, etc.)

Resources:

Online sample preparation guides

Workflows & Protocols Plant Laser Microdissection
Protocol LMD of cryo-sectioned Brain Tissue for Proteomics
Protocol LMD of FFPE Brain Tissue for Proteomics

9/19/2025 Webinar describing system capabilities, applications, workflows, and a broad overview of sample preparation

PPT slides (pdf)

Leica LMD7 brochure (pdf)
Lots of documents related to LMD operation and applications

Workshop--Single Molecule Localization Microscopy and TIRF
2025-11-03 08:00 - 2025-11-07 17:00
Test out the Abbelight SMLM and TIRF system
Taylor Hall 229
Co-hosted by Jenn Marks with Evident Scientific (formerly Olympus).
See the system working with one of the demo slides or test your own sample.
Sign up for a time slot with the 👉 Doodle Sign-Up Sheet
For maximal success with your samples, contact Paul Barthélémy as soon as possible to ensure you have the optimal sample holders and fluorophores (pdf). We have a small amount of secondary antibodies with appropriate fluorophores available on-site for sample preparation prior to your imaging session.
Watch the UNC Sample Prep Webinar from Oct. 9, 2025.
Practical notes:
1: SMLM will not work with your standard confocal sample of a coverslip mounted on a slide with mounting media using whichever fluorophores you have in your freezer. The preparation is very similar to your confocal protocol, but there are a few extremely critical details that might differ. You will likely need to generate a specific sample for the demo.
2: Fluorophores needed for SMLM also work great for standard fluorescence applications, so anything you might need to buy for a successful demo can likely be used for other applications.
PDF product brochure

Spatial Biology & Single-Cell Seminar presented by Bruker
2025-11-12 10:00 - 15:00
10am-3pm MBRB
Details and free registration
https://go.brukerspatialbiology.com/UNC-SingleCellSymposium-REG.html

QuPath working group - Intro organizational meeting
2026-02-20 12:00 - 13:00
In person: MEJ 3102 — feel free to bring your lunch
Online: Zoom link
Come learn about what QuPath can do and help us structure on-campus resources for learning how to use this powerful image analysis software too.

Introductions – Wendy/Shannon
What is QuPath? (very brief); why is it different than other open-source (or UNC paid for softwares)
Why are we here? Why imaging/analysis folks think this is important
Anticipated activities throughout the semester from our POV

Workshop/demo (1 hr), Co-working time – talk to each other to solve issues, “Data club” type: presentations of data/analysis/problems

How to get help:

Teams group for “help” calls – or something similar; what is best way to contact everyone, forum.Image.sc, Contact information for us

How can we support you? Brainstorming session

Introduction to QuPath for Whole Slide Image Analysis
2026-03-10 08:00 - 17:00
QuPath from an Experts Perspective
Sara McArdle, PhD
Sr. Staff Scientist, Microscopy Core, La Jolla Institute for Immunology
Virtual seminar
Live group viewing MEJ 3106
Online https://go.unc.edu/Jg25X

QuPath working group
2026-03-10 08:00 - 17:00
MEJ 3102
Specific topic TBD
