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SUMMARY:Workshop--Single Molecule Localization Microscopy and TIRF
DESCRIPTION:Test out the Abbelight SMLM and TIRF system\nTaylor Hall 229\nCo-hosted by Jenn Marks with Evident Scientific (formerly Olympus). \nSee the system working with one of the demo slides or test your own sample. \nSign up for a time slot with the 👉 Doodle Sign-Up Sheet \nFor maximal success with your samples\, contact Paul Barthélémy as soon as possible to ensure you have the optimal sample holders and fluorophores (pdf). We have a small amount of secondary antibodies with appropriate fluorophores available on-site for sample preparation prior to your imaging session. \nWatch the UNC Sample Prep Webinar from Oct. 9\, 2025. \nPractical notes:\n1: SMLM will not work with your standard confocal sample of a coverslip mounted on a slide with mounting media using whichever fluorophores you have in your freezer. The preparation is very similar to your confocal protocol\, but there are a few extremely critical details that might differ. You will likely need to generate a specific sample for the demo.\n2: Fluorophores needed for SMLM also work great for standard fluorescence applications\, so anything you might need to buy for a successful demo can likely be used for other applications. \nPDF product brochure
URL:https://www.med.unc.edu/hic-imaging/event/workshop-single-molecule-localization-microscopy-and-tirf/
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