{"id":2250,"date":"2023-11-20T14:37:02","date_gmt":"2023-11-20T19:37:02","guid":{"rendered":"https:\/\/www.med.unc.edu\/hic-imaging\/?page_id=2250"},"modified":"2024-09-24T15:06:05","modified_gmt":"2024-09-24T19:06:05","slug":"leica-stellaris-8-falcon-sted","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/hic-imaging\/instrumentation\/leica-stellaris-8-falcon-sted\/","title":{"rendered":"Leica STELLARIS 8 FALCON STED"},"content":{"rendered":"<h3><img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-2708 size-medium\" src=\"https:\/\/www.med.unc.edu\/hic-imaging\/wp-content\/uploads\/sites\/1291\/2024\/05\/1000002489-scaled-e1715366188778-300x300.jpg\" alt=\"Leica Stellaris 8 FALCON STED\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.med.unc.edu\/hic-imaging\/wp-content\/uploads\/sites\/1291\/2024\/05\/1000002489-scaled-e1715366188778-300x300.jpg 300w, https:\/\/www.med.unc.edu\/hic-imaging\/wp-content\/uploads\/sites\/1291\/2024\/05\/1000002489-scaled-e1715366188778-1024x1024.jpg 1024w, https:\/\/www.med.unc.edu\/hic-imaging\/wp-content\/uploads\/sites\/1291\/2024\/05\/1000002489-scaled-e1715366188778-150x150.jpg 150w, https:\/\/www.med.unc.edu\/hic-imaging\/wp-content\/uploads\/sites\/1291\/2024\/05\/1000002489-scaled-e1715366188778-768x768.jpg 768w, https:\/\/www.med.unc.edu\/hic-imaging\/wp-content\/uploads\/sites\/1291\/2024\/05\/1000002489-scaled-e1715366188778-1536x1536.jpg 1536w, https:\/\/www.med.unc.edu\/hic-imaging\/wp-content\/uploads\/sites\/1291\/2024\/05\/1000002489-scaled-e1715366188778-600x600.jpg 600w, https:\/\/www.med.unc.edu\/hic-imaging\/wp-content\/uploads\/sites\/1291\/2024\/05\/1000002489-scaled-e1715366188778.jpg 1800w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/h3>\n<p>A laser scanning confocal on an inverted <a href=\"https:\/\/www.leica-microsystems.com\/products\/confocal-microscopes\/p\/stellaris-8\/\">Leica <\/a>DMI8 motorized microscope stand<\/p>\n<h2>Features<\/h2>\n<ul>\n<li><a href=\"https:\/\/www.leica-microsystems.com\/science-lab\/stellaris-white-light-lasers\/\">White-light laser<\/a> for optimal excitation from 440nm-790nm plus 405nm diode. Easy 5+ channel imaging!<\/li>\n<li>3D <a href=\"https:\/\/www.leica-microsystems.com\/products\/confocal-microscopes\/p\/stellaris-sted\/\">STED<\/a> super-resolution with 775nm depletion beam for resolution down to 50nm or smaller. <strong>NOTE<\/strong>: STED specific information <a href=\"#STED-specific-resources\">below<\/a><a href=\"https:\/\/www.leica-microsystems.com\/science-lab\/the-guide-to-sted-sample-preparation\/\">.<\/a><\/li>\n<li><a href=\"https:\/\/www.leica-microsystems.com\/science-lab\/life-science\/tausense-technology-imaging-tools\/\">TauSense<\/a> modules that use lifetime information for filtering<\/li>\n<li>FALCON for accurate lifetime measurements.<\/li>\n<li>Sensitive <a href=\"https:\/\/www.leica-microsystems.com\/science-lab\/life-science\/power-hyd-detectors\/\">hybrid detectors<\/a> are capable of photon counting and enable time-gating to reduce autofluorescence background.<\/li>\n<li>Live cell stage top incubator. NOTE: Requires 35mm dish or chambered coverslips.<\/li>\n<li>AFC (Automatic Focus Correction) to prevent focal drift<\/li>\n<\/ul>\n<p><!-- notionvc: 91086397-aa86-424a-a7c1-d50a8117ee08 --><\/p>\n<hr \/>\n<h2>Acknowledgement<\/h2>\n<p>Acknowledgement of NIH grant 1S10OD030300 is required for all publications in which data from this instrument is used.<!-- notionvc: 84bfb085-8cb1-4b35-9c98-ff0b030ffaf5 --><\/p>\n<hr \/>\n<h2>Location: 234D Taylor Hall<\/h2>\n<hr \/>\n<h2>Specifications<\/h2>\n<h3>Objectives<\/h3>\n<ul>\n<li>HC Plan Fluotar 5x\/0.15 WD13.7mm<\/li>\n<li>HC Plan-Apo 10x\/0.45 WD2.0mm<\/li>\n<li>Plan-Apo 20x\/0.8WD 0.55mm<\/li>\n<li>Plan-Neofluar 40x\/1.3 oil WD0.21<\/li>\n<li>Plan-Apo 63x\/1.4 oil (partial list)<\/li>\n<\/ul>\n<h3>Excitation Lines<\/h3>\n<ul>\n<li>405nm<\/li>\n<li>440-790 continuous selection with White Light Laser<\/li>\n<\/ul>\n<h3>Detection<\/h3>\n<ul>\n<li>3 HyD S detectors<\/li>\n<li>2 HyD X detectors<\/li>\n<li>Transmitted light detector (DIC)<\/li>\n<\/ul>\n<h3><a id=\"STED-specific-resources\"><\/a>STED specific resources<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.med.unc.edu\/hic-imaging\/wp-content\/uploads\/sites\/1291\/2024\/09\/STED-sample-prep-for-HIC.docx\">STED sample prep for HIC<\/a> document includes links below and some UNC-specific links.<\/li>\n<li>Do NOT use AlexaFluor 647; it photobleaches almost instantly in STED mode.<\/li>\n<li>Leica&#8217;s <a href=\"https:\/\/downloads.leica-microsystems.com\/STELLARIS%208%20STED\/Application%20Note\/STELLARIS%20STED%20Sample%20Preparation%20Guide.pdf\">STED sample preparation guide (pdf download)<\/a>, including fluorophore guide<\/li>\n<li><a href=\"https:\/\/abberior.rocks\/\">Abberior&#8217;s<\/a>\u00a0document on <a href=\"https:\/\/content.ilabsolutions.com\/wp-content\/uploads\/2021\/03\/STED-Sample-Preparation-and-Labelling-MGV-2020-10-12.pdf\">STED sample preparation (pdf download)<\/a><\/li>\n<li>We have had success getting STED resolution in 3 colors with AlexaFluor 594, ATTO 647N and CF680R (<a href=\"https:\/\/biotium.com\/\">Biotium<\/a>).<\/li>\n<li>For UNC researchers, the HIC has a small bank of reagents for testing STED imaging on your samples. Please contact Wendy for details.<\/li>\n<li><strong>Places to buy antibodies with STED fluorophores (weblinks, not exhaustive): <\/strong>PLEASE email <a href=\"mailto:wendy_salmon@med.unc.edu\">Wendy<\/a> other sources!\n<ul>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/products\/protein-biology\/antibodies?msockid=35b78a2bc56165133bfa99a0c1616b59\">Milipore-Sigma<\/a> has some standard secondaries with aberrior dyes<\/li>\n<li><a href=\"https:\/\/www.rockland.com\/categories\/secondary-antibodies\/\">Rockland<\/a> has IgG subtype specific antibodies<\/li>\n<li><a href=\"https:\/\/www.creative-biolabs.com\/sdab\/category-secondary-sdab-312.htm\">Creative Labs<\/a> has camelid nanobodies (smaller, less amplification)<\/li>\n<li><a href=\"https:\/\/biotium.com\/technology\/primary-secondary-antibody-conjugates\/secondary-antibody-product-listings\/\">Biotium<\/a> (CF dyes) has standard secondary host-target pairs plus donkey-raised secondaries and secondaries against less-common primary hosts (guinea pig, rat, etc). Lists <a href=\"https:\/\/biotium.com\/technology\/cf-dyes\/cf-dyes-for-super-resolution-microscopy\/#references\">STED compatible dyes.<\/a><\/li>\n<li><a href=\"https:\/\/www.jacksonimmuno.com\/\">Jackson ImmunoResearch<\/a> for AlexaFluor 594<\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/brands\/molecular-probes.html\">ThermoFisher\/Molecular Probes<\/a> for AlexaFluor 594<\/li>\n<li>Label your own antibodies with dye kits: <a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/products\/protein-biology\/antibodies?msockid=35b78a2bc56165133bfa99a0c1616b59\">Sigma<\/a> (Aberrior dyes, US), <a href=\"https:\/\/www.atto-tec.com\/produkte\/Fluorescent-Labels\/350-nm---500-nm\/\">Atto-Tec<\/a> (Aberrior dyes, EU) and <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/brands\/molecular-probes.html\">ThermoFisher\/Molecular Probes<\/a> (AlexaFluors)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><!-- notionvc: 3efa7abc-1488-4b7a-be07-e980a00d0af8 --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A laser scanning confocal on an inverted Leica DMI8 motorized microscope stand Features White-light laser for optimal excitation from 440nm-790nm plus 405nm diode. Easy 5+ channel imaging! 3D STED super-resolution with 775nm depletion beam for resolution down to 50nm or smaller. NOTE: STED specific information below. TauSense modules that use lifetime information for filtering FALCON &hellip; <a href=\"https:\/\/www.med.unc.edu\/hic-imaging\/instrumentation\/leica-stellaris-8-falcon-sted\/\" aria-label=\"Read more about Leica STELLARIS 8 FALCON STED\">Read more<\/a><\/p>\n","protected":false},"author":111865,"featured_media":0,"parent":2571,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2250","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Leica STELLARIS 8 FALCON STED - Hooker Imaging Core<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/hic-imaging\/instrumentation\/leica-stellaris-8-falcon-sted\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Leica STELLARIS 8 FALCON STED - Hooker Imaging Core\" \/>\n<meta property=\"og:description\" content=\"A laser scanning confocal on an inverted Leica DMI8 motorized microscope stand Features White-light laser for optimal excitation from 440nm-790nm plus 405nm diode. Easy 5+ channel imaging! 3D STED super-resolution with 775nm depletion beam for resolution down to 50nm or smaller. NOTE: STED specific information below. 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