The Lenti-shRNA Core Facility is premised in Open Biosystems TRC1 shRNA libraries.<\/span><\/b><\/p>\n The libraries are cataloged as a single lentiviral-vector shRNA expression clones directed to +\/- 16,000 human and mouse genes. Each gene is targeted by 4 or 5 different shRNAs driven by the U6 promoter. The shRNA Core provides the lentiviral shRNA vectors in four different formats: Lenti cDNA Library now available.<\/span><\/b><\/p>\n The lenti-shRNA core now has available a lenti-cDNA library. Welcome to the Lenti-shRNA Core Facility The Lenti-shRNA Core Facility is premised in Open Biosystems TRC1 shRNA libraries. The libraries are cataloged as a single lentiviral-vector shRNA expression clones directed to +\/- 16,000 human and mouse genes. Each gene is targeted by 4 or 5 different shRNAs driven by the U6 promoter. Please note that … Read more<\/a><\/p>\n","protected":false},"author":1179,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"templates\/front-page.php","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2210","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/lenti-shrna\/wp-json\/wp\/v2\/pages\/2210","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/lenti-shrna\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/lenti-shrna\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/lenti-shrna\/wp-json\/wp\/v2\/users\/1179"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/lenti-shrna\/wp-json\/wp\/v2\/comments?post=2210"}],"version-history":[{"count":1,"href":"https:\/\/www.med.unc.edu\/lenti-shrna\/wp-json\/wp\/v2\/pages\/2210\/revisions"}],"predecessor-version":[{"id":2365,"href":"https:\/\/www.med.unc.edu\/lenti-shrna\/wp-json\/wp\/v2\/pages\/2210\/revisions\/2365"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/lenti-shrna\/wp-json\/wp\/v2\/media?parent=2210"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nPlease note that the shRNA knockdown efficiency has not been validated.<\/span><\/p>\n
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\nSeveral clones in this library have been tested and demonstrated efficient cDNA expression by Western blot analysis. The cDNAs are expressed under the control of a CMV promoter. An additional PGK promoter controls the expression of a Blasticidin resistance selection marker.
\nAll the cDNAs are V5-tagged.
\nThe cDNAs were incorporated into the lenti cassette via the gateway system. Thus, the relevant cDNAs can be mobilized via the gateway system to gateway-compatible cassettes.<\/p>\n","protected":false},"excerpt":{"rendered":"