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Jonathan Fay, PhD

Assistant Professor

Specialty Areas:

Single particle cryo-electron microscopy (cryoEM); membrane protein biochemistry; pharmacology; and site-directed fluorescent labeling techniques

Research Focus:

Dr. Fay’s work seeks to gain atomic insights into the biochemical function of ion channels and membrane proteins in airway epithelium. His research utilizes various techniques to interrogate protein structure and dynamics to provide fundamental insights into the molecular underpinnings of how these proteins function in both healthy and diseased states.

Selected Bibliography:

  1. Fay JF, Aleksandrov LA, Jensen TJ, Cui LL, Kousouros JN, He L, Aleksandrov AA, Gingerich DS, Riordan JR, Chen JZ. Cryo-EM Visualization of an Active High Open Probability CFTR Anion Channel. Biochemistry. 2018 Oct 30;57(43):6234-6246. doi: 10.1021/acs.biochem.8b00763. PMID: 30281975.
  2. Aleksandrov LA, Fay JF, Riordan JR. R-Domain Phosphorylation by Protein Kinase A Stimulates Dissociation of Unhydrolyzed ATP from the First Nucleotide-Binding Site of the Cystic Fibrosis Transmembrane Conductance Regulator. Biochemistry. 2018 Aug 28;57(34):5073-5075. doi: 10.1021/acs.biochem.8b00646. PMID: 30109929.
  3. Walsh SM, Mathiasen S, Christensen SM, Fay JF, King C, Provasi D, Borrero E, Rasmussen SGF, Fung JJ, Filizola M, Hristova K, Kobilka B, Farrens DL, Stamou D. Single Proteoliposome High-Content Analysis Reveals Differences in the Homo-Oligomerization of GPCRs. Biophys J. 2018 Jul 17;115(2):300-312. doi: 10.1016/j.bpj.2018.05.036. PMID: 30021106; PMCID: PMC6050755.
  4. Martin GM, Yoshioka C, Rex EA, Fay JF, Xie Q, Whorton MR, Chen JZ, Shyng SL. Cryo-EM structure of the ATP-sensitive potassium channel illuminates mechanisms of assembly and gating. Elife. 2017 Jan 16;6:e24149. doi: 10.7554/eLife.24149. PMID: 28092267; PMCID: PMC5344670.
Jonathan Fay