{"id":468,"date":"2011-11-22T17:20:00","date_gmt":"2011-11-22T22:20:00","guid":{"rendered":"https:\/\/med.sites.unc.edu\/cfpulmcenter\/collaborative-opportunities\/pre-clinical-capabilities-1\/cell-biology-of-pulmonary-epithelia\/"},"modified":"2025-03-21T08:52:37","modified_gmt":"2025-03-21T12:52:37","slug":"cell-biology-of-pulmonary-epithelia","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/cell-biology-of-pulmonary-epithelia\/","title":{"rendered":"Cell Biology of Pulmonary Epithelia"},"content":{"rendered":"<p><!-- portlet_header --><\/p>\n<p><!-- portlet_above --><\/p>\n<p><!-- description --><\/p>\n<p><a name=\"top\"><\/a>The Marsico Lung Institute has an extensive supply of well-characterized human airway epithelial culture preparations. Indeed, the Institute has a stock of more than 200 individual subject-derived upper (nasal) and lower airway (tracheobronchial) culture stocks from normal subjects, CF subjects, and subjects with COPD. These cultures can be grown in a variety of systems, most typically our air-liquid interface system (<strong>Fig. 1<\/strong>).<\/p>\n<p>Recently, the Institute has extended its cell purification\/preparation capacities to other cell types within the lung. Consequently, we now have available stocks of human alveolar type II cells (<strong>Fig. 2<\/strong>) and human pulmonary endothelial cells (<strong>Fig. 3<\/strong>). Upon request, we also have the capability of isolating human AT1 cells for specific studies.<\/p>\n<table style=\"width: 500px\" border=\"0\">\n<tbody>\n<tr>\n<th><img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 1\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-1.png\" alt=\"Microscopy image of human lung primary bronchial epithelial cells in air liquid interface cultures\" width=\"250\" height=\"162\" \/><\/th>\n<th><img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 2\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-2.png\" alt=\"Microscopy images of human lung primary alveolar type II cells, and PCR images showing gene expression\" width=\"250\" height=\"225\" \/><\/th>\n<\/tr>\n<tr>\n<td><span class=\"somoverlay smallFont discreet\"><strong>Figure 1. Human lung primary bronchial epithelial cells in air liquid interface cultures.<\/strong> The cells were prepared for histology using perflurocarbon-OsO<sub>4<\/sub> to preserve the apical periciliary layer and overlying mucus. <\/span><\/td>\n<td valign=\"top\"><span class=\"somoverlay smallFont discreet\"><strong>Figure 2. Human lung primary alveolar type II cells.<\/strong> <strong>A.<\/strong> Freshly isolated cells stained for SPC (ii) and Aqp3 (iii). <strong>B.<\/strong> Cells at air-liquid interface. C,D. PCR for gene expression. <\/span><\/td>\n<\/tr>\n<tr>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td><img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 3A\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-3A.png\" alt=\"Two graphs, one depicting Ab control and the other depicting Anti-CD31\" width=\"250\" height=\"251\" \/><\/td>\n<td><img loading=\"lazy\" decoding=\"async\" class=\"image-inline alignnone\" title=\"Figure 3B\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-3B.png\" alt=\"Magnified image of human lung primary microvascular endothelial cells\" width=\"250\" height=\"251\" \/><\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\"><span class=\"discreet\"><span class=\"smallFont\"><strong>Figure 3. Human lung primary microvascular endothelial cells.<\/strong> Greater than 98% are positive for CD31 and the cells make tight monolayers suitable for permeability and transport studies.<\/span> <\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>The Cell Biology Core has the capacity to produce growth-extended cells. Such approaches include novel &#8220;immortalizing&#8221; genetic approaches, which produce cells with greatly extended growth capacity and good preservation of phenotypic structure and function (<strong>Fig. 4<\/strong>). In addition, more recent techniques, including fibroblast feeder cells and &#8220;Y compounds,&#8221; have been used to growth-extend both human airway epithelial cells and alveolar epithelial cells, and are available upon request.<\/p>\n<table style=\"width: 400px\" border=\"0\">\n<tbody>\n<tr>\n<th><img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 4\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-4.png\" alt=\"Magnified images of growth-enhanced human primary bronchial epithelial cells\" width=\"400\" height=\"228\" \/><\/th>\n<\/tr>\n<tr>\n<td><span class=\"discreet smallFont\"><strong>Figure 4. Novel growth enhanced human primary bronchial epithelial cells.<\/strong> These cells (3 normal and 3 CF donors) are capable of pseudostratified mucociliary differentiation after repeated passaging and are particularly useful for testing and applying genetic manipulation strategies.<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n","protected":false},"excerpt":{"rendered":"<p>The Marsico Lung Institute has an extensive supply of well-characterized human airway epithelial culture preparations. Indeed, the Institute has a stock of more than 200 individual subject-derived upper (nasal) and lower airway (tracheobronchial) culture stocks from normal subjects, CF subjects, and subjects with COPD. These cultures can be grown in a variety of systems, most &hellip; <a href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/cell-biology-of-pulmonary-epithelia\/\" aria-label=\"Read more about Cell Biology of Pulmonary Epithelia\">Read more<\/a><\/p>\n","protected":false},"author":76119,"featured_media":0,"parent":60,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-468","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Cell Biology of Pulmonary Epithelia | Marsico Lung Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/cell-biology-of-pulmonary-epithelia\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cell Biology of Pulmonary Epithelia | Marsico Lung Institute\" \/>\n<meta property=\"og:description\" content=\"The Marsico Lung Institute has an extensive supply of well-characterized human airway epithelial culture preparations. Indeed, the Institute has a stock of more than 200 individual subject-derived upper (nasal) and lower airway (tracheobronchial) culture stocks from normal subjects, CF subjects, and subjects with COPD. 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