{"id":473,"date":"2011-11-22T17:20:00","date_gmt":"2011-11-22T22:20:00","guid":{"rendered":"https:\/\/med.sites.unc.edu\/cfpulmcenter\/collaborative-opportunities\/pre-clinical-capabilities-1\/integrated-mucus-transport-activities-of-airway-epithelia\/"},"modified":"2025-03-21T13:31:58","modified_gmt":"2025-03-21T17:31:58","slug":"integrated-mucus-transport-activities-of-airway-epithelia","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/integrated-mucus-transport-activities-of-airway-epithelia\/","title":{"rendered":"Integrated Mucus Transport Activities of Airway Epithelia"},"content":{"rendered":"<p><!-- portlet_header --><\/p>\n<p><!-- portlet_above --><\/p>\n<p><!-- description --><\/p>\n<p><a name=\"top\"><\/a>The Marsico Lung Institute has extensive experience measuring the integrated pulmonary defense activity of mucus transport in both culture and small animal models. <span style=\"line-height: 1.43em\">With respect to culture, we have long had in-house our \u201cclassic\u201d system whereby cell cultures grown in 1.2 mm Transwell ALI culture systems develop spontaneous rotational mucus transport that can be quantitated by time-lapse fluorescence videomicroscopy (<\/span><b style=\"line-height: 1.43em\">Fig. 13<\/b><span style=\"line-height: 1.43em\">). These techniques have been supplemented recently with novel techniques designed to utilize in-house built culture systems to measure mucus transport rates in linear systems. <\/span><span style=\"line-height: 1.43em\">The in vitro systems are complemented by a number of techniques to measure mucus transport in mouse models. These techniques include direct deposition of radiotracers or fluorescence tracers, followed by measurement of linear clearance rates with time in the trachea (<\/span><b style=\"line-height: 1.43em\">Fig. 14<\/b><span style=\"line-height: 1.43em\">) or nasopharynx (<\/span><b style=\"line-height: 1.43em\">Fig. 15<\/b><span style=\"line-height: 1.43em\">). In addition, techniques have been developed to deposit aerosols on pulmonary surfaces to measure whole lung mucus clearance, using external technetium radioisotope counting techniques (<\/span><b style=\"line-height: 1.43em\">Fig. 16<\/b><span style=\"line-height: 1.43em\">).<\/span><\/p>\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-6 col-md-6 col-xs-6 col-sm-6 oscitas-bootstrap-container\">\n<img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 13\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-13.png\" alt=\"Time-lapse fluorescence microscopy image of air-liquid interface culture surface\" width=\"535\" height=\"386\" \/><\/p>\n<p><span class=\"discreet\"><b>Figure 13. <\/b>Time lapse fluorescence microscopy of air-liquid interface culture surface to measure mucus transport rates.<\/span><\/div>\n<div class=\"col-lg-6 col-md-6 col-xs-6 col-sm-6 oscitas-bootstrap-container\">\n<img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 15\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/figure15.png\" alt=\"Fluorescence tracer image, more fully described in the figure caption\" width=\"381\" height=\"253\" \/><\/p>\n<p><span class=\"discreet\"><b>Figure 14.<\/b> Mucociliary clearance in the murine posterior nasopharynx (soft palate) following aspiration of 6um fluorescent beads<\/span><span class=\"discreet\">.<\/span><\/div>\n<\/div>\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-6 col-md-6 col-xs-6 col-sm-6 oscitas-bootstrap-container\">\n<img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 14\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/figure14.png\" alt=\"Fluorescent tracer image, described more fully in the figure caption\" width=\"240\" height=\"179\" \/><\/p>\n<p><span class=\"discreet\"><b><span style=\"color: #000000\">Figure 15.<\/span> <\/b>Mucociliary clearance (MCC) in the murine trachea following aspiration of fluorescent beads (6 um) in perflurocarbon (0.25 ul\/g). The trachea of the anesthetized mouse is exposed and covered with water equilibrated mineral oil for imaging. The first images of MCC are obtained while the mouse is alive (the breathing motions are obvious) and then the anesthetized mouse is euthanized by exsanguination and MCC is again recorded. Images are obtained by placing the mouse under a fluorescent dissection microscope (Leica) and recording with a DAGE MTI video camera.<\/span><\/div>\n<div class=\"col-lg-6 col-md-6 col-xs-6 col-sm-6 oscitas-bootstrap-container\">\n<img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 16\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/figure16.png\" alt=\"Fluorescence microscopy image\" width=\"225\" height=\"224\" \/><\/p>\n<p><span class=\"discreet\"><b>Figure 16. <\/b>Time lapse fluorescence microscopy of air-liquid interface culture surface to measure mucus transport rates.<\/span><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Marsico Lung Institute has extensive experience measuring the integrated pulmonary defense activity of mucus transport in both culture and small animal models. With respect to culture, we have long had in-house our \u201cclassic\u201d system whereby cell cultures grown in 1.2 mm Transwell ALI culture systems develop spontaneous rotational mucus transport that can be quantitated &hellip; <a href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/integrated-mucus-transport-activities-of-airway-epithelia\/\" aria-label=\"Read more about Integrated Mucus Transport Activities of Airway Epithelia\">Read more<\/a><\/p>\n","protected":false},"author":76119,"featured_media":0,"parent":60,"menu_order":22,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-473","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Integrated Mucus Transport Activities of Airway Epithelia | Marsico Lung Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/integrated-mucus-transport-activities-of-airway-epithelia\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Integrated Mucus Transport Activities of Airway Epithelia | Marsico Lung Institute\" \/>\n<meta property=\"og:description\" content=\"The Marsico Lung Institute has extensive experience measuring the integrated pulmonary defense activity of mucus transport in both culture and small animal models. With respect to culture, we have long had in-house our \u201cclassic\u201d system whereby cell cultures grown in 1.2 mm Transwell ALI culture systems develop spontaneous rotational mucus transport that can be quantitated &hellip; Read more\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/integrated-mucus-transport-activities-of-airway-epithelia\/\" \/>\n<meta property=\"og:site_name\" content=\"Marsico Lung Institute\" \/>\n<meta property=\"article:modified_time\" content=\"2025-03-21T17:31:58+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-13.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" 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