{"id":478,"date":"2011-11-22T17:20:00","date_gmt":"2011-11-22T22:20:00","guid":{"rendered":"https:\/\/med.sites.unc.edu\/cfpulmcenter\/collaborative-opportunities\/pre-clinical-capabilities-1\/ion-liquid-transport\/"},"modified":"2025-03-21T09:07:22","modified_gmt":"2025-03-21T13:07:22","slug":"ion-liquid-transport","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/ion-liquid-transport\/","title":{"rendered":"Ion\/Liquid Transport"},"content":{"rendered":"<p><!-- portlet_header --><\/p>\n<p><!-- portlet_above --><\/p>\n<p><!-- description --><\/p>\n<p><a name=\"top\"><\/a>The Marsico Lung Institute also has extensive capabilities to perform classical Ussing chamber studies on large numbers of tissues for screening purposes. Extensive experience has accrued in developing the appropriate quality control procedures to assure accurate and reproducible testing of compound activities in this assay.<\/p>\n<p>The Institute also has extensive experience in characterizing airway surface liquid physiology, in response to candidate therapeutic agents, under the \u201cthin film\u201d ASL technique (<strong>Fig. 6<\/strong>). In \u201cstandard\u201d versions of this technique, a combination of airway surface liquid (ASL) volume as measured by confocal techniques, indices of ion transport processes mediating ASL volume homeostasis from transepithelial microelectrode voltage measurements, coupled with micropipette liquid sampling for measurements of drug concentrations, can be made. Note, drugs can be added to the basolateral compartment of these preparations, the luminal compartment in perfluorocarbon, or indeed, more recently, by an interface to ultrasonic nebulizers that deliver aerosols to cultured airway surfaces at rates that mimic aerosol deposition<i> in vivo<\/i> (e.g., 20 nl\/cm<sup>2<\/sup>\/min \u2013 <strong>Fig. 7<\/strong>).<\/p>\n<p>&nbsp;<\/p>\n<figure class=\"thumbnail wp-caption alignnone\" style=\"width: 260px\"><img loading=\"lazy\" decoding=\"async\" class=\"somoverlay\" title=\"Figure 6\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-6.png\" alt=\"Confocal micrograph image described in figure caption\" width=\"250\" height=\"169\" \/><figcaption class=\"caption wp-caption-text\"><strong>Figure 6.<\/strong> X-z confocal micrograph of airway surface liquid (red) atop well-differentiated HBE culture (green).<\/figcaption><\/figure>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 7A\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-7A.png\" alt=\"Graph with Deposition Rate as the Y-axis and Generation as the X-axis\" width=\"236\" height=\"171\" \/><img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 7B\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-7B.png\" alt=\"Photograph of a nano-liter volume nebulization system with its components labeled\" width=\"239\" height=\"168\" \/><br \/>\n<span class=\"discreet\"><strong><span style=\"color: #000000\">Figure 7.<\/span> <\/strong>Interface of HBE cultures with aerosol delivery of agents. <strong>A.<\/strong> The average deposition rate (nanoliters per square cm per minute of deposition) over the first 20 airway generations in humans, comparing the rates of two commercially available nebulizers. <strong>B.<\/strong> Nano-liter volume nebulization system positioned for drug deposition to the apical surface of human bronchial epithelial cells.<\/span><\/p>\n<table style=\"width: 750px\" border=\"0\">\n<tbody>\n<tr>\n<td><img loading=\"lazy\" decoding=\"async\" class=\"somoverlay alignnone\" title=\"Figure 8\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-8.png\" alt=\"Graph depicting the diffusion of microbeads through HBE mucus\" width=\"225\" height=\"168\" \/><\/td>\n<\/tr>\n<tr>\n<td><span class=\"discreet\"><strong><span style=\"color: #000000\">Figure 8.<\/span> <\/strong>Diffusion of microbeads through HBE mucus. Movement [i.e., mean squared displacement, \u0394r<sup>2<\/sup>(m<sup>2<\/sup>)] ~1\u03bc particle is faster in normal 1% mucus than CF-like, dehydrated\/thickened mucus.<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>The Marsico Lung Institute also has extensive capabilities to perform classical Ussing chamber studies on large numbers of tissues for screening purposes. Extensive experience has accrued in developing the appropriate quality control procedures to assure accurate and reproducible testing of compound activities in this assay. The Institute also has extensive experience in characterizing airway surface &hellip; <a href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/ion-liquid-transport\/\" aria-label=\"Read more about Ion\/Liquid Transport\">Read more<\/a><\/p>\n","protected":false},"author":76119,"featured_media":0,"parent":60,"menu_order":16,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-478","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Ion\/Liquid Transport | Marsico Lung Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/ion-liquid-transport\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Ion\/Liquid Transport | Marsico Lung Institute\" \/>\n<meta property=\"og:description\" content=\"The Marsico Lung Institute also has extensive capabilities to perform classical Ussing chamber studies on large numbers of tissues for screening purposes. Extensive experience has accrued in developing the appropriate quality control procedures to assure accurate and reproducible testing of compound activities in this assay. 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