{"id":491,"date":"2011-11-22T17:20:00","date_gmt":"2011-11-22T22:20:00","guid":{"rendered":"https:\/\/med.sites.unc.edu\/cfpulmcenter\/collaborative-opportunities\/pre-clinical-capabilities-1\/molecular-biology-of-pulmonary-epithelium\/"},"modified":"2025-03-21T09:02:42","modified_gmt":"2025-03-21T13:02:42","slug":"molecular-biology-of-pulmonary-epithelium","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/molecular-biology-of-pulmonary-epithelium\/","title":{"rendered":"Molecular Biology of Pulmonary Epithelium"},"content":{"rendered":"<p><!-- portlet_header --><\/p>\n<p><!-- portlet_above --><\/p>\n<p><!-- description --><\/p>\n<p><a name=\"top\"><\/a>The Marsico Lung Institute has extensive capabilities in the molecular biology of human airway epithelia. With respect to the<a class=\"internal-link\" href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/cell-biology-of-pulmonary-epithelia\"> cell stocks mentioned<\/a>, most stocks have been characterized for CFTR status and are available for other genetic studies. Specific molecular capabilities now include:<\/p>\n<ul>\n<li><i>Studies of gene expression in human pulmonary epithelia<\/i>: Available are techniques and data that have emanated from extensive Affymetric analyses of whole genome expression in human airway and alveolar epithelia, and also more recent data generated from high throughput sequencing (454 pyrosequencing) of RNA to produce \u201cRNA seq\u201d libraries of human pulmonary epithelia under a variety of conditions.<\/li>\n<li><i>Gene transduction capacities in pulmonary epithelia:<\/i> These capacities include the ability to express both conventional transgenes, siRNAs, and shRNAs in human pulmonary epithelia. The vectors of choice for these techniques include equine infectious anemia virus (EIAV) gene transfer and shRNA expression (<strong>Fig. 5<\/strong>), paramyxovirus (RSV\/PIV3) vectors, AAV vectors, adenoviral vectors, and lipids\/plasma formulations.<\/li>\n<\/ul>\n<hr \/>\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-6 col-md-12 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\n<p>&nbsp;<\/p>\n<figure id=\"attachment_492\" class=\"thumbnail wp-caption alignnone\" style=\"width: 332px\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-492 size-full\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-5A.png\" alt=\"Graphs, western blot images, and immunofluorescent staining images depicting what is described more fully in the figure caption\" width=\"322\" height=\"302\" srcset=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-5A.png 322w, https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-5A-300x281.png 300w\" sizes=\"auto, (max-width: 322px) 100vw, 322px\" \/><figcaption class=\"caption wp-caption-text\"><strong>Figure 5.<\/strong> Expertise in genetic manipulation of hTBE. <strong>Top Panel:<\/strong> VSV-G pseudotyped pSLIK-based (G418 resistance) lentiviral vector was used express shRNA to the ENaC protein in an inducible manner [induced by doxycycline (Dox)]. Primary hTBE cells on plastic were infected with pSLIK lentivirus, selected with G418, subcultured at an air liquid interface to induce differentiation and induced with Dox. <strong>A)<\/strong> &gt; 50% mRNA reduction was seen +Dox compared to -Dox. <strong>B)<\/strong> This corresponded to a &gt;80% reduction in ENaC protein by western blots, <strong>C)<\/strong> a corresponding reduction in ENaC function (amiloride) by Ussing chambers, and <strong>D)<\/strong> an increase in airway surface liquid height representing decreased ENaC activity.<\/figcaption><\/figure><\/div>\n<div class=\"col-lg-6 col-md-12 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\n<figure id=\"attachment_493\" class=\"thumbnail wp-caption alignnone\" style=\"width: 349px\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-493 size-full\" src=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-5B.png\" alt=\"Magnified images demonstrating molecular biology capabilities\" width=\"339\" height=\"114\" srcset=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-5B.png 339w, https:\/\/www.med.unc.edu\/marsicolunginstitute\/wp-content\/uploads\/sites\/547\/2017\/12\/Figure-5B-300x101.png 300w\" sizes=\"auto, (max-width: 339px) 100vw, 339px\" \/><figcaption class=\"caption wp-caption-text\">Inducible over-expression of red fluorescent protein (RFP) was obtained in a similar fashion using inducible lentiviral vector (pTRIPZ). The images show whole mounts stained with anti-beta tubulin IV for ciliated cells (green) and red fluorescence. RFP expression is robust and only in the presence of Dox (3D confocal stack).<\/figcaption><\/figure>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Marsico Lung Institute has extensive capabilities in the molecular biology of human airway epithelia. With respect to the cell stocks mentioned, most stocks have been characterized for CFTR status and are available for other genetic studies. Specific molecular capabilities now include: Studies of gene expression in human pulmonary epithelia: Available are techniques and data &hellip; <a href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/molecular-biology-of-pulmonary-epithelium\/\" aria-label=\"Read more about Molecular Biology of Pulmonary Epithelium\">Read more<\/a><\/p>\n","protected":false},"author":76119,"featured_media":0,"parent":60,"menu_order":12,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-491","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Molecular Biology of Pulmonary Epithelium | Marsico Lung Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/molecular-biology-of-pulmonary-epithelium\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Molecular Biology of Pulmonary Epithelium | Marsico Lung Institute\" \/>\n<meta property=\"og:description\" content=\"The Marsico Lung Institute has extensive capabilities in the molecular biology of human airway epithelia. 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Expertise in genetic manipulation of hTBE.\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/molecular-biology-of-pulmonary-epithelium\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Marsico Lung Institute Accomplishments\",\"item\":\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Pre-Clinical Opportunities\",\"item\":\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Molecular Biology of Pulmonary Epithelium\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/#website\",\"url\":\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/\",\"name\":\"Marsico Lung Institute\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Molecular Biology of Pulmonary Epithelium | Marsico Lung Institute","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.med.unc.edu\/marsicolunginstitute\/collaborative-opportunities\/pre-clinical-capabilities-1\/molecular-biology-of-pulmonary-epithelium\/","og_locale":"en_US","og_type":"article","og_title":"Molecular Biology of Pulmonary Epithelium | Marsico Lung Institute","og_description":"The Marsico Lung Institute has extensive capabilities in the molecular biology of human airway epithelia. 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