{"id":608,"date":"2015-09-25T15:17:25","date_gmt":"2015-09-25T19:17:25","guid":{"rendered":"https:\/\/med.sites.unc.edu\/cfpulmcenter\/spirovation\/pre-clinical-capabilities\/"},"modified":"2023-01-11T11:13:36","modified_gmt":"2023-01-11T16:13:36","slug":"pre-clinical-capabilities","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/marsicolunginstitute\/spirovation\/pre-clinical-capabilities\/","title":{"rendered":"Pre-clinical Capabilities"},"content":{"rendered":"<p><!-- portlet_above --><\/p>\n<p><!-- description --><\/p>\n<h2 class=\" \">The Tissue Procurement and Cell Culture Core<\/h2>\n<h3 class=\" \">Director: Scott H. Randell, PhD<\/h3>\n<h4>Capabilities<\/h4>\n<div class=\"O1\">\u2022Tissue Procurement<\/div>\n<div class=\"O1\">\u2022Lung Cell Isolation and Culture, Including Primary Airway and Alveolar Epithelium, Microvascular Endothelial Cells and Fibroblasts<\/div>\n<div class=\"O1\">\u2022Specialized Media Formulations<\/div>\n<div class=\"O1\">\u2022In vivo and in vitro Airway Surface Materials<\/div>\n<div class=\"O1\">\u2022Genetic Manipulation of Lung Cell Cultures<\/div>\n<div class=\"O1\">\u2022Creation and Characterization of Novel Cell Lines<\/div>\n<h2>The Molecular Biology Core<\/h2>\n<h3>Director: Wanda K O&#8217;Neal, PhD<\/h3>\n<h4>Capabilities<\/h4>\n<div>\u2022The Core serves as a centralized resource for cloning and sequencing of genes.<\/div>\n<div>\u2022Isolation, storage, and analysis of high quality RNA.<\/div>\n<div>\u2022Generation of transgenic mouse models.<\/div>\n<div>\u2022Target validation&#8211; development of methods for si\/shRNA, CRISPR knockdown in airway cell models.<\/div>\n<div>\u2022Immunohistochemistry.<\/div>\n<div>\u2022Provide training in molecular biology.<\/div>\n<h2>The Histology Core<\/h2>\n<h4>Capabilities<\/h4>\n<div class=\"O1\">\u2022Light microscopy services include: prosection, tissue fixation, processing, embedding, sectioning, staining and cover slipping of paraffin, frozen and soft plastic tissue sections.<\/div>\n<div class=\"O1\">\u2022The histology core also provides expertise in all aspects of transmission and scanning electron microscopy. Access to a high pressure freezer and automated freeze substitution is available.<\/div>\n<div class=\"O1\">\u2022A highlight of the core facility is our specialized procedure for histological evaluation of airway cells grown on porous membrane supports.<\/div>\n<h2>CFTR Functional Analysis Core <span style=\"line-height: 1.43em\">and Cystic Fibrosis Molecular\/Functional Measurement Core<\/span><\/h2>\n<h3><span style=\"line-height: 1.43em\">Director: Martina Gentzsch, PhD<\/span><\/h3>\n<h4>Capabilities<\/h4>\n<div>\u2022In vitro short-circuit current measurements to assess ion channel properties in primary and conditionally reprogrammed bronchial and nasal epithelial cells from patients (CF, COPD etc.) and controls.<\/div>\n<div>\u2022Ex vivo measurements of ion transport function in rectal biopsies from patients and controls.<\/div>\n<div>\u2022In vivo NPD, salivary secretion, and mucociliary clearance measurements in mouse models.<\/div>\n<div>\u2022Analysis of CFTR protein expression and biochemical correction of mutant CFTR.<\/div>\n<div>\u2022Assessment of efficacy of functional rescue of mutant CFTR by pharmacological agents.<\/div>\n<h2>Microscopy Facility<\/h2>\n<h4>Capabilities<\/h4>\n<div class=\"O1\">\u2022Advanced and Standard Microscopy<\/div>\n<div class=\"O1\">\u2022Confocal Microscopy<\/div>\n<div class=\"O1\">\u2022Live Cell Imaging<\/div>\n<div class=\"O1\">\u2022Color Brightfield Imaging (high resolution)<\/div>\n<div class=\"O1\">\u2022Stereo Fluorescence Micro\/Macroscope<\/div>\n<div class=\"O1\">\u2022Laser Micro-Dissection System<\/div>\n<div class=\"O1\">\u2022Image Processing and Analysis<\/div>\n<h2>The Animal Models Core<\/h2>\n<h3>Director: Wanda K O&#8217;Neal, PhD<br \/>\nCo-Director: Dr. Alessandra Livraghi-Butrico, PhD<\/h3>\n<h4>Capabilities<\/h4>\n<div>\u2022The Mouse Models Lab has developed and maintains active colonies of various mouse models relevant for basic and translational pulmonary research, i.e., \u03b2ENaC (Scnn1b) transgenic, \u0394F508 Cftr mice, secreted and tethered Mucin-deficient mice, and relative intercrosses. Models of acute lung injury, sepsis, pneumonia\/infection, cigarette smoke exposure are also available.<\/div>\n<div>\u2022Phenotyping: bronchoalveolar lavage (neonates and adults), differential cell counts and inflammatory markers, histopathology and morphometry, secreted mucins agarose western blots, airway mucus burden, microbiology, airway mucus clearance measurements.<\/div>\n<div>\u2022Administration of pharmacologic treatments via intra-tracheal instillation, nasal aspiration, gavage, subcute or intraperitoneal injection, in neonatal or adult mice.<\/div>\n<h2>Center for Airways Disease<\/h2>\n<h3>Director: Claire Doerschuk, MD<\/h3>\n<h4>Capabilities<\/h4>\n<div class=\"O1\">\u2022Recruitment of leukocytes during pneumonia and other lung injuries.<\/div>\n<div class=\"O1\">\u2022Endothelial cell biology in normal lungs and during inflammatory and innate immune processes.<\/div>\n<div class=\"O1\">\u2022Cigarette-smoke induced lung disease.<\/div>\n<div class=\"O1\">\u2022Effector functions of leukocytes.<\/div>\n<div class=\"O1\">\u2022The function of the small GTPase Rac2 expressed in endothelial cells which regulates both normal fluid flux and edema formation during injury.<\/div>\n<h2>Mucociliary Transport (MCT) and Mucus Clearance<\/h2>\n<h4>Capabilities<\/h4>\n<div>\u2022In-vitro functionality<\/div>\n<div class=\"O1\" style=\"padding-left: 30px\">\u2013Airway Surface Liquid (ASL) depth, mucociliary transport, ciliary beat frequency<\/div>\n<div class=\"O1\" style=\"padding-left: 30px\">\u2013Mucus secretion\/production<\/div>\n<div class=\"O1\" style=\"padding-left: 30px\">\u2013Penetration kinetics of drugs through mucus<\/div>\n<div class=\"O1\" style=\"padding-left: 30px\">\u2013Mucus rheology, biophysics and clearance<\/div>\n<div class=\"O1\" style=\"padding-left: 30px\">\u2013Tools for mucolytic drug screening<\/div>\n<div>\u2022Mouse in-vivo MCT<\/div>\n<div>\u2022MCT in human volunteers<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Tissue Procurement and Cell Culture Core Director: Scott H. Randell, PhD Capabilities \u2022Tissue Procurement \u2022Lung Cell Isolation and Culture, Including Primary Airway and Alveolar Epithelium, Microvascular Endothelial Cells and Fibroblasts \u2022Specialized Media Formulations \u2022In vivo and in vitro Airway Surface Materials \u2022Genetic Manipulation of Lung Cell Cultures \u2022Creation and Characterization of Novel Cell Lines &hellip; <a href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/spirovation\/pre-clinical-capabilities\/\" aria-label=\"Read more about Pre-clinical Capabilities\">Read more<\/a><\/p>\n","protected":false},"author":57194,"featured_media":0,"parent":140,"menu_order":12,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-608","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Pre-clinical Capabilities | Marsico Lung Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/spirovation\/pre-clinical-capabilities\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Pre-clinical Capabilities | Marsico Lung Institute\" \/>\n<meta property=\"og:description\" content=\"The Tissue Procurement and Cell Culture Core Director: Scott H. 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