Seeing Molecules Up Close – Superresolution
Reading Assignment:
Good general reference:
http://zeiss-campus.magnet.fsu.edu/articles/superresolution/index.html
Introduction to Superresolution-Zeiss.pdf (or http://zeiss-campus.magnet.fsu.edu/articles/superresolution/introduction.html)
The possibility of getting around the diffraction limit in light microscopy.
- Super Resolution by Spot Engineering (STED)
Far-Field Optical Nanoscopy.pdf (or http://www.sciencemag.org/content/316/5828/1153.long)
- Super Resolution by Fluorophor Engineering (PALM)
Introduction to PALM – Zeiss.pdf (or http://zeiss-campus.magnet.fsu.edu/articles/superresolution/palm/introduction.html)
- Super Resolution by Light Pattern Engineering (structured illumination)
Simple Interactive Tutorial:
http://zeiss-campus.magnet.fsu.edu/tutorials/superresolution/hrsim/index.html
In-depth article by Gustafsson et al:
http://www.sciencedirect.com/science/article/pii/S0006349508703606