Why Is Resolution Limited By Diffraction?
Reading Assignment:
Path 464 Class Notes Ch 4-Optics, Ch 7-Lenses, Ch-13 Confocal – section on Magnification, Resolution and Sampling; section on Advantages of CLSM
Diffraction See: Image Formation.pdf
The nature of an edge
The effect of wavelength
The effect of refractive index
The limits of Numerical Aperture See: Numerical Aperture and Resolution.pdf
Wide field versus confocal resolution
- Resolution in the x/y-axis versus the z-axis
- Confocal resolution is limited by diffraction
Circumventing the diffraction limit: More on this at the end of the course
- Super Resolution by Spot Engineering (STED)
- Super Resolution by Fluorophore Engineering (PALM)
- Super Resolution by Light Pattern Engineering (structured illumination)