{"id":2289,"date":"2023-11-03T16:54:21","date_gmt":"2023-11-03T20:54:21","guid":{"rendered":"https:\/\/www.med.unc.edu\/microscopy\/?page_id=2289"},"modified":"2025-12-08T10:54:59","modified_gmt":"2025-12-08T15:54:59","slug":"electron-microscopy","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/microscopy\/electron-microscopy\/","title":{"rendered":"Electron Microscopy"},"content":{"rendered":"<h2>How we will help you<\/h2>\n<p>At MSL we will maximize your chances of success by:<\/p>\n<ul>\n<li><strong>Consulting<\/strong> with you about your project<\/li>\n<li><strong>Preparing<\/strong> your samples for electron microscopy<\/li>\n<li><strong>Taking high quality images of your samples<\/strong> using our transmission and\/or scanning electron microscopes<\/li>\n<\/ul>\n<p>Please <a href=\"mailto:msl@med.unc.edu;kristen_white@med.unc.edu;jillann_madren@med.unc.edu\">contact us<\/a> as early as possible in your project. We support projects that require transmission electron microscopy (TEM) and scanning electron microscopy (SEM).<\/p>\n<hr class=\" rule-thick osc-rule\" \/>\n<h2>Sample preparation services<\/h2>\n<p><strong>Consult our flowchart below for what to expect:<\/strong><\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/12\/website-em-flowchart.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2937 size-large\" src=\"https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/website-em-flowchart-1024x562.png\" alt=\"Flowchart showing sample preparation services depending on users client's needs; linking to flowchart pdf.\" width=\"1024\" height=\"562\" srcset=\"https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/website-em-flowchart-1024x562.png 1024w, https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/website-em-flowchart-300x165.png 300w, https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/website-em-flowchart-768x421.png 768w, https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/website-em-flowchart-1536x843.png 1536w, https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/website-em-flowchart-2048x1124.png 2048w, https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/website-em-flowchart-600x329.png 600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/p>\n<p>MSL can prepare samples using the following techniques:<\/p>\n<ul>\n<li>Standard sample preparation for transmission electron microscopy<\/li>\n<li>Standard sample preparation for scanning electron microscopy<\/li>\n<li>Negative staining<\/li>\n<li>Immunoelectron microscopy &#8211; please read <a href=\"https:\/\/microscopy.jhmi.edu\/services\/immuno-em\/\" target=\"_blank\" rel=\"noopener\">this<\/a> for context<\/li>\n<li>Correlative light and electron microscopy<\/li>\n<\/ul>\n<hr class=\" rule-thick osc-rule\" \/>\n<h2>Equipment<\/h2>\n<div class=\"panel panel-info  oscitas-bootstrap-container\">\n<div class=\"panel-heading oscitas-bootstrap-container\">\n<h3>Transmission Electron Microscope \u2013 JEOL JEM 1400 Flash &#8211; &#8220;Albus&#8221;<\/h3>\n<p class=\"lead\">Ideal for imaging nanometer resolution subcellular ultrastructure of biological materials.<\/p>\n<\/div>\n<div class=\"panel-body oscitas-bootstrap-container\">\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-large wp-image-3844\" src=\"https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2025\/06\/IMG_0822-scaled.jpg\" alt=\"\" width=\"1024\" height=\"768\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\n<ul>\n<li>Magnification 10x to 1,200,000x<\/li>\n<li>Resolution: 0.2 nm, high contrast objective pole piece<\/li>\n<li>Operating voltage: 80kV &#8211; 120kV<\/li>\n<li>Tungsten filament<\/li>\n<li>Equipped with AMT NanoSprint 61 ActiveVu camera<\/li>\n<\/ul>\n<p><em>Usually operated by MSL staff. Training may be available, upon request, depending on duration of project.<\/em><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"panel panel-info  oscitas-bootstrap-container\">\n<div class=\"panel-heading oscitas-bootstrap-container\">\n<h3>Scanning Electron Microscope \u2013 Zeiss SUPRA 25<\/h3>\n<p class=\"lead\">Ideal for surface imaging of biological materials at high magnification.<\/p>\n<\/div>\n<div class=\"panel-body oscitas-bootstrap-container\">\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-12 oscitas-bootstrap-container\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2790\" src=\"https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/Supra-SEM.jpg\" alt=\"Scanning Electron Microscope\" width=\"311\" height=\"414\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\n<ul>\n<li>Magnification: 20x to 1,000,000x<\/li>\n<li>Resolution: 1.7 nm @ 15 kV<\/li>\n<li>Detectors:\n<ul>\n<li>with standard T-E (secondary electron SE2)<\/li>\n<li>InLens<\/li>\n<li>backscatter detector (BSD)<\/li>\n<li>EDX<\/li>\n<li>Scanning transmission electron microscopy detector (STEM)<\/li>\n<\/ul>\n<\/li>\n<li>Motorized stage holds up to 8 standard samples or 12 STEM samples.<\/li>\n<li>Accelerating voltages 0.2kV to 30 KV<\/li>\n<li>Stage rotation: 360\u00b0<\/li>\n<li>Stage tilt:\u202f0-90 degrees<\/li>\n<li>Denka TFE Cathode Tip<\/li>\n<\/ul>\n<p><em>Usually operated by MSL staff. Training may be available, upon request, depending on duration of project.<\/em><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"panel panel-info  oscitas-bootstrap-container\">\n<div class=\"panel-heading oscitas-bootstrap-container\">\n<h3>Critical Point Dryer \u2013 Tousimis Samdri-795<\/h3>\n<p class=\"lead\">Used for drying water-containing samples with minimal shrinkage and artifacts.<\/p>\n<\/div>\n<div class=\"panel-body oscitas-bootstrap-container\">\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-12 oscitas-bootstrap-container\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2790\" src=\"https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/CPD-Tousimis.jpg\" alt=\"Critical Point Dryer\" width=\"311\" height=\"414\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\nCustomized large sample chamber compatible with various sample holders<\/p>\n<p><em>Typically operated by MSL staff. Training may be available, upon request.<\/em><\/p>\n<a class=\"btn btn-default btn-lg  oscitas-bootstrap-container\" target=\"_blank\" href=\"https:\/\/youtu.be\/PyvCt8eBu4E?si=EGbzI7rbL_64133c\"><i class=\"fa-solid fa-film\" ><\/i> CPD training video<\/a>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"panel panel-info  oscitas-bootstrap-container\">\n<div class=\"panel-heading oscitas-bootstrap-container\">\n<h3>Sputter coater \u2013 Cressington 208HR<\/h3>\n<p class=\"lead\">High resolution sputtering for field emission SEM with gold-palladium or chromium.<\/p>\n<\/div>\n<div class=\"panel-body oscitas-bootstrap-container\">\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-12 oscitas-bootstrap-container\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2790\" src=\"https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/Sputter-Cressington-300x229.jpg\" alt=\"Sputter coater\" width=\"311\" height=\"414\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\n<ul>\n<li>High resolution sputtering for field emission SEM with gold-palladium or chromium.<\/li>\n<li>Equipped with quartz crystal to continuously monitor coating thickness\/li&gt;<\/li>\n<li>Rotary stage for even distribution of coating materials<\/li>\n<li>Adjustable stage tilt during processing<\/li>\n<li>Stage that holds 24 SEM pin stubs<\/li>\n<\/ul>\n<p><em>Typically operated by MSL staff. Training may be available, upon request.<\/em><\/p>\n<a class=\"btn btn-default btn-lg  oscitas-bootstrap-container\" target=\"_blank\" href=\"https:\/\/youtu.be\/rva7TwXkxxw?si=mB2v6tNcAjm_5Mq9\"><i class=\"fa-solid fa-film\" ><\/i> Sputter coater training video<\/a>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<hr class=\" rule-thin osc-rule\" \/>\n<h2>Retired Equipment<\/h2>\n<div role=\"tablist\" class=\"panel-group yourcustomclass oscitas-bootstrap-container\" id=\"unc-accordion-0\">\n\n<div class=\"panel panel-default oscitas-bootstrap-container\">\n\n    <div id=\"panel-heading-details-0-0\" class=\"panel-heading oscitas-bootstrap-container\" role=\"tab\">\n\n        <h3 class=\"panel-title oscitas-bootstrap-container\">\n\n            <button class=\"accordion-toggle oscitas-bootstrap-container collapsed\" \n                id = \"button-details-0-0\"\n                data-toggle=\"collapse\"\n                data-parent=\"#unc-accordion-0\"    \n                aria-controls=\"#details-0-0\"  \n                aria-expanded=\"false\" \n                href=\"#details-0-0\">\n                Transmission Electron Microscope \u2013 JEOL JEM 1230            <\/button>\n\n        <\/h3>\n\n    <\/div>\n\n    <div id=\"details-0-0\" class=\"panel-collapse collapse  oscitas-bootstrap-container\">\n\n        <div class=\"panel-body oscitas-bootstrap-container\">\n<p>This microscope was retired in May of 2025. We include its information here only for reference.<\/p>\n<div class=\"row  oscitas-bootstrap-container\">\n<div class=\"col-lg-4 col-md-4 col-xs-12 col-sm-12 oscitas-bootstrap-container\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2790\" src=\"https:\/\/www.med.unc.edu\/microscopy\/wp-content\/uploads\/sites\/1393\/2023\/11\/JEOL-1230-1.jpg\" alt=\"Transmission Electron Microscope\" width=\"311\" height=\"414\" \/><\/div>\n<div class=\"col-lg-8 col-md-8 col-xs-12 col-sm-12 oscitas-bootstrap-container\">\n<ul>\n<li>Magnification 50x to 600,000x<\/li>\n<li>Resolution: 0.2 nm lattice, 0.36 nm point resolution, high contrast objective pole piece<\/li>\n<li>Operating voltage: 80kV<\/li>\n<li>Tungsten filament<\/li>\n<li>Equipped with Gatan Orius SC1000 CCD camera with Gatan Microscopy Suite version 3.10.1002.0 software<\/li>\n<\/ul>\n<p><em>Usually operated by MSL staff. Training may be available, upon request, depending on duration of project.<\/em><\/div>\n<\/div>\n<\/div>\n\n    <\/div>\n        \n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>How we will help you At MSL we will maximize your chances of success by: Consulting with you about your project Preparing your samples for electron microscopy Taking high quality images of your samples using our transmission and\/or scanning electron microscopes Please contact us as early as possible in your project. We support projects that &hellip; <a href=\"https:\/\/www.med.unc.edu\/microscopy\/electron-microscopy\/\" aria-label=\"Read more about Electron Microscopy\">Read more<\/a><\/p>\n","protected":false},"author":112631,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2289","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/pages\/2289","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/users\/112631"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/comments?post=2289"}],"version-history":[{"count":12,"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/pages\/2289\/revisions"}],"predecessor-version":[{"id":3870,"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/pages\/2289\/revisions\/3870"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/media?parent=2289"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}