{"id":2354,"date":"2023-11-08T09:46:02","date_gmt":"2023-11-08T14:46:02","guid":{"rendered":"https:\/\/www.med.unc.edu\/microscopy\/?page_id=2354"},"modified":"2024-01-03T16:15:46","modified_gmt":"2024-01-03T21:15:46","slug":"eight-steps-to-rigorous-and-reproducible-experiments-in-biomolecular-research-at-unc","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/microscopy\/resources\/rigor-and-reproducibility\/eight-steps-to-rigorous-and-reproducible-experiments-in-biomolecular-research-at-unc\/","title":{"rendered":"Eight steps to Rigorous and Reproducible Experiments in Biomolecular Research at UNC"},"content":{"rendered":"<ol>\n<li>If using a core facility,\u00a0<strong>consult\u00a0<\/strong>with the core staff in the planning stage. Consult with a statistician if you need help developing a Power Analysis to assure that your results will be adequately powered.<\/li>\n<li>Design your experiment with\u00a0<strong>sufficient controls<\/strong>\u00a0(rigor)\u00a0<strong>and replicates <\/strong>(reproducibility).<\/li>\n<li>Assure that ALL of your reagents (antibodies, cell lines, mice) are<strong> fully validated<\/strong>.<\/li>\n<li>Have a clear and\u00a0<strong>detailed protoco<\/strong>l (SOP) and data analysis plan. Assure that the protocol is strictly followed or that any deviation is well documented.<\/li>\n<li>Assure that the staff or students performing the experiment are\u00a0<strong>well trained<\/strong>\u00a0and understand each step and the importance of performing them precisely.<\/li>\n<li>Use only\u00a0<strong>well-maintained instrumentation<\/strong>, preferably maintained and operated in a core facility with expert staff (see #1 above).<\/li>\n<li><strong>Document all steps<\/strong>, reagents, equipment and data analysis methods used in the experiment. Assure that the both the documentation and the data itself are properly stored in a safe data management repository.<\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/microscopy\/new-users\/publication-acknowledgements\/\"><strong>Acknowledge<\/strong><\/a>\u00a0the Cancer Center Support Grant (<em>P30 CA016086) (if applicable)<\/em>, other grants that support the core, the core (by name), and core staff in publications.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>If using a core facility,\u00a0consult\u00a0with the core staff in the planning stage. Consult with a statistician if you need help developing a Power Analysis to assure that your results will be adequately powered. Design your experiment with\u00a0sufficient controls\u00a0(rigor)\u00a0and replicates (reproducibility). Assure that ALL of your reagents (antibodies, cell lines, mice) are fully validated. Have a &hellip; <a href=\"https:\/\/www.med.unc.edu\/microscopy\/resources\/rigor-and-reproducibility\/eight-steps-to-rigorous-and-reproducible-experiments-in-biomolecular-research-at-unc\/\" aria-label=\"Read more about Eight steps to Rigorous and Reproducible Experiments in Biomolecular Research at UNC\">Read more<\/a><\/p>\n","protected":false},"author":58247,"featured_media":0,"parent":2312,"menu_order":7,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2354","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/pages\/2354","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/users\/58247"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/comments?post=2354"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/pages\/2354\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/pages\/2312"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/microscopy\/wp-json\/wp\/v2\/media?parent=2354"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}