MiniMUGA Sample Report

MiniMUGA Sample Report - V2

Sample ID

B6;FVB-Apoc2em1Arem/Mmnc_M_31715

Neogen ID

AK4761

MMRRC Strain ID

42203

MMRRC Strain Name

B6N;FVB-Apoc2^em1Arem/Mmnc

Summary

The genotype of this sample is of excellent quality. It is male and outbred, and likely a mix of multiple C57BL/6 substrains and FVB/NCrl. Clustering of unexplained markers is evidence of an additional background strain.

Diagnostic SNPs indicate the presence of the background strain groups C57BL/6 and the substrains C57BL/6J, C57BL/6NRj, FVB/NCrl.

No genetic constructs were detected in this sample.

Genotyping Quality

Excellent (11 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Outbred (736 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
multiple C57BL/6 substrains	9719	7600 (82.6%)	873 (9.5%)	730 (7.9%)	516

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
FVB/NCrl	1603 Clustered	1547 (16.8%) 1507 Clustered	54 (0.6%) 48 Clustered	2 (0.0%)

Y Chromosome

Y Haplogroup 12 - 100.0% Consistent
Includes B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 14 other strains

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac, FVB/NCrl and 150 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
C57BL/6NRj	5	2	20	35.0%
FVB/NCrl	6	3	47	19.1%
C57BL/6J	5	0	156	3.2%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac)	7	2	21	42.9%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
multiple C57BL/6 substrains	1207	43.0%	47.6%
FVB/NCrl	839	29.9%	28.7%
Heterozygous	708	25.2%	22.3%
Unexplained Homozygous	34	1.2%	0.9%
Unexplained Heterozygous	20	0.7%	0.5%
Total	2808	100.0%	100.0%

Refined Ideogram

Notes

The sample is outbred and contains diagnostic markers from C57BL/6NRj, FVB/NCrl, and C57BL/6J. There is also evidence of an additional background strain.