MiniMUGA Sample Report

MiniMUGA Sample Report - V2

Sample ID

MMRRC_UNC_M38182

Neogen ID

DE6262

MMRRC Strain ID

42299

MMRRC Strain Name

129S;B6-Tg(CAG-H3f3a/EGFP)1Dean/Mmnc

Summary

The genotype of this sample is of excellent quality. It is male and outbred, and likely a mix of multiple C57BL/6 substrains and FVB/NCrl. Clustering of unexplained markers is evidence of an additional background strain.

Diagnostic SNPs indicate the presence of the background substrains B6N-Tyr/BrdCrCrl, C57BL/6J, FVB/NCrl.

The sample contains the following genetic constructs: "Greenish" Fluorescent Protein (EGFP, EYFP, ECFP), iCre recombinase, Internal Ribosome Entry Site

Genotyping Quality

Excellent (12 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Outbred (1571 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
multiple C57BL/6 substrains	9719	7211 (78.4%)	464 (5.0%)	1526 (16.6%)	518

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
FVB/NCrl	1990 Clustered	1936 (21.0%) 1906 Clustered	53 (0.6%) 44 Clustered	1 (0.0%)

Y Chromosome

Y Haplogroup 14 - 100.0% Consistent
Includes C57BL/6J and 10 other strains

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac, FVB/NCrl and 150 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
B6N-Tyr/BrdCrCrl	1	14	31	48.4%
FVB/NCrl	6	15	47	44.7%
C57BL/6J	2	15	156	10.9%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
multiple C57BL/6 substrains	817	29.1%	30.0%
FVB/NCrl	464	16.5%	19.8%
Heterozygous	1472	52.5%	49.4%
Unexplained Homozygous	0	0.0%	0.0%
Unexplained Heterozygous	53	1.9%	0.8%
Total	2806	100.0%	100.0%

Refined Ideogram

Notes

The sample is outbred and diagnostic markers indicate the presence of C57BL6N, FVBNCrl, and C57BL/6J. G_GFP, IRES, and iCre constructs are detected. iCre is not expected.