MiniMUGA Sample Report

MiniMUGA Sample Report - Initial Version

Sample ID

C57BL/6J-Tg(GFAP-CHRM3*)6Kdmc/Mmnc_M_32201

Neogen ID

EM0574

MMRRC Strain ID

42286

MMRRC Strain Name

C57BL/6J-Tg(GFAP-CHRM3*)6Kdmc/Mmnc

Summary

The genotype of this sample is of excellent quality. It is male and inbred, and likely a C57BL/6J mouse.

Diagnostic SNPs indicate the presence of the background strain groups C57BL/6 and the substrains C57BL/6J.

No genetic constructs were detected in this sample.

Genotyping Quality

Excellent (6 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Inbred (3 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
C57BL/6J	9719	9583 (100.0%)	0 (0.0%)	0 (0.0%)	136

Secondary Background
(Autosomes,
X Chromosome)

Not Applicable

Y Chromosome

Y Haplogroup 14 - 100.0% Consistent
Includes C57BL/6J and 10 other strains

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes C57BL/6J and 161 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
C57BL/6J	148	1	156	95.5%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (B6N-Tyr/BrdCrCrl, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd)	6	0	21	28.6%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
C57BL/6J	9583	100.0%	100.0%
Heterozygous	0	0.0%	0.0%
Unexplained Homozygous	0	0.0%	0.0%
Unexplained Heterozygous	0	0.0%	0.0%
Total	9583	100.0%	100.0%

Refined Ideogram

Notes

The report agrees with strain nomenclature and the SDS.