MiniMUGA Sample Report

MiniMUGA Sample Report - Refined Analysis

Sample ID

MMRRC_UNC_M40219

Neogen ID

FZ8322

MMRRC Strain ID

43918

MMRRC Strain Name

129S6;129P2-Grin1^tm1Bhk/Mmnc

Summary

The genotype of this sample is of excellent quality. It is male and inbred, and likely a mix of 129S6/SvEvTac and 129P2/OlaHsd.

Diagnostic SNPs indicate the presence of the background strain groups 129S.

No genetic constructs were detected in this sample.

Genotyping Quality

Excellent (6 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Inbred (1 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
129S6/SvEvTac	9719	9585 (100.0%)	0 (0.0%)	1 (0.0%)	133

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
129P2/OlaHsd	1 Clustered	1 (0.0%) 0 Clustered	0 (0.0%) 0 Clustered	0 (0.0%)

Y Chromosome

Y Haplogroup 6 - 98.4% Consistent
Includes 129P2/OlaHsd, 129S6/SvEvTac and 10 other strains

MT Chromosome

MT Haplogroup 6 - 100.0% Consistent
Includes 129S6/SvEvTac and 12 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

Strain Group	Homozygous	Heterozygous	Potential	% Observed
129S (129S1/SvImJ, 129S2/SvHsd, 129S2/SvPasOrlRj, 129S4/SvJaeJ, 129S6/SvEvTac)	10	0	17	58.8%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
129S6/SvEvTac	695	99.9%	99.4%
129P2/OlaHsd	0	0.0%	0.0%
Heterozygous	1	0.1%	0.6%
Unexplained Homozygous	0	0.0%	0.0%
Unexplained Heterozygous	0	0.0%	0.0%
Total	696	100.0%	100.0%

Refined Ideogram

Notes

The report agrees with strain nomenclature and the SDS.