MiniMUGA Sample Report

MiniMUGA Sample Report - V2

Sample ID

B6.129S2-Rbp1tm1Ipc/Mmucd_M_2L2R

Neogen ID

HX5727

MMRRC Strain ID

41471

MMRRC Strain Name

B6.129S2-Rbp1^tm1Ipc/Mmucd

Summary

The genotype of this sample is of excellent quality. It is male and close to inbred, and likely a mix of multiple C57BL/6 substrains and 129S2/SvHsd.

Diagnostic SNPs indicate the presence of the background strain groups C57BL/6 and the substrains B6N-Tyr/BrdCrCrl, C57BL/6J.

The sample contains the following genetic constructs: Bovine growth hormone poly A signal sequence

Genotyping Quality

Excellent (37 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Close to Inbred (76 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
multiple C57BL/6 substrains	9719	9132 (99.5%)	0 (0.0%)	45 (0.5%)	542

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
129S2/SvHsd	45 Clustered	41 (0.4%) 30 Clustered	4 (0.0%) 0 Clustered	0 (0.0%)

Y Chromosome

Y Haplogroup 14 - 100.0% Consistent
Includes C57BL/6J and 10 other strains

MT Chromosome

MT Haplogroup 1 - 98.9% Consistent
Includes 129S2/SvHsd, B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 150 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
C57BL/6J	131	19	156	96.2%
B6N-Tyr/BrdCrCrl	0	4	31	12.9%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (B6N-Tyr/BrdCrCrl, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NRj)	5	2	21	33.3%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
multiple C57BL/6 substrains	2792	98.4%	98.2%
129S2/SvHsd	0	0.0%	0.0%
Heterozygous	41	1.4%	1.6%
Unexplained Homozygous	0	0.0%	0.0%
Unexplained Heterozygous	4	0.1%	0.2%
Total	2837	100.0%	100.0%

Refined Ideogram

Notes

The sample is close to inbred and the report is consistent with the SDS and strain nomenclature. bpA construct is detected.