MiniMUGA Sample Report

MiniMUGA Sample Report - V2

Sample ID

B6J.B6N-Tmem35atm1(KOMP)Vlcg/TranMmnc_F_34941

Neogen ID

IM0269

MMRRC Strain ID

43504

MMRRC Strain Name

B6J.B6N-Tmem35a^{tm1(KOMP)Vlcg}/TranMmnc

Summary

The genotype of this sample is of excellent quality. It is female and outbred, and likely a mix of multiple C57BL/6 substrains and multiple FVB/N substrains. Clustering of unexplained markers is evidence of an additional background strain.

Diagnostic SNPs indicate the presence of the background strain groups C57BL/6 and the substrains B6N-Tyr/BrdCrCrl, C57BL/6J, C57BL/6NRj, FVB/NCrl, FVB/NJ.

No genetic constructs were detected in this sample.

Genotyping Quality

Excellent (12 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Outbred (799 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
multiple C57BL/6 substrains	9719	7794 (84.7%)	629 (6.8%)	777 (8.4%)	519

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
multiple FVB/N substrains	1406 Clustered	1188 (12.9%) 1185 Clustered	205 (2.2%) 202 Clustered	13 (0.1%)

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 151 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
C57BL/6NRj	4	8	20	60.0%
B6N-Tyr/BrdCrCrl	1	3	31	12.9%
FVB/NCrl	4	1	47	10.6%
FVB/NJ	3	5	79	10.1%
C57BL/6J	1	3	156	2.6%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac)	6	3	21	42.9%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
multiple C57BL/6 substrains	1517	52.1%	56.7%
multiple FVB/N substrains	575	19.8%	17.6%
Heterozygous	613	21.1%	19.9%
Unexplained Homozygous	47	1.6%	1.6%
Unexplained Heterozygous	158	5.4%	4.1%
Total	2910	100.0%	100.0%

Refined Ideogram

Notes

The SDS indicates that the strain was backcrossed 10G on C57BL/6J, although the report indicates that there is significant FVBand C57BL/6N remaining in the genome . Diagnostics for C57BL/6J were detected but at a much lower level than expected relative to C57BL/6N and FVB diagnostics. There is also evidence of an additonal strain.