MiniMUGA Sample Report

MiniMUGA Sample Report - V2

Sample ID

B6J.B6N-Tmem35atm1(KOMP)Vlcg/TranMmnc_M_34942

Neogen ID

MM1024

MMRRC Strain ID

43504

MMRRC Strain Name

B6J.B6N-Tmem35a^{tm1(KOMP)Vlcg}/TranMmnc

Summary

The genotype of this sample is of excellent quality. It is male and outbred, and likely a mix of multiple C57BL/6 substrains and multiple FVB/N substrains. Clustering of unexplained markers is evidence of an additional background strain.

Diagnostic SNPs indicate the presence of the background strain groups C57BL/6 and the substrains C57BL/6J, C57BL/6NRj, FVB/NCrl, FVB/NJ.

No genetic constructs were detected in this sample.

Genotyping Quality

Excellent (24 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Outbred (756 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
multiple C57BL/6 substrains	9719	7925 (86.2%)	527 (5.7%)	737 (8.0%)	530

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
multiple FVB/N substrains	1264 Clustered	1105 (12.0%) 1102 Clustered	145 (1.6%) 135 Clustered	14 (0.2%)

Y Chromosome

Y Haplogroup 1 - 100.0% Consistent

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 151 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
C57BL/6NRj	5	5	20	50.0%
FVB/NCrl	0	6	47	12.8%
FVB/NJ	4	5	79	11.4%
C57BL/6J	0	3	156	1.9%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac)	9	3	21	57.1%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
multiple C57BL/6 substrains	1591	56.0%	62.9%
multiple FVB/N substrains	493	17.4%	14.3%
Heterozygous	612	21.5%	18.7%
Unexplained Homozygous	30	1.1%	1.0%
Unexplained Heterozygous	115	4.0%	3.0%
Total	2841	100.0%	100.0%

Refined Ideogram

Notes

The SDS indicates that the strain was backcrossed 10G on C57BL/6J, although the report indicates that the mouse is outbred. Diagnostics for C57BL/6J were detected but at a much lower level than expected relative to C57BL/6N and FVB diagnostics. Similar results are obtained for the other sample that was genotyped for this strain.