MiniMUGA Sample Report

MiniMUGA Sample Report - V2

Sample ID

MMRRC_B6.Cg-Casz1tm1.1Flc/Mmnc_M23362

Neogen ID

MU5665

MMRRC Strain ID

41181

MMRRC Strain Name

B6.Cg-Casz1^{tm2(cre/ERT2)Flc}/Mmnc

Summary

The genotype of this sample is of excellent quality. It is male and close to inbred, and likely a mix of multiple C57BL/6 substrains and 129P2/OlaHsd.

Diagnostic SNPs indicate the presence of the background strain groups C57BL/6 and the substrains C57BL/6J.

The sample contains the following genetic constructs: Bovine growth hormone poly A signal sequence, Cre recombinase, hCMV enhancer version a, hCMV enhancer version b, Internal Ribosome Entry Site, "Reddish" fluorescent protein (tdTomato, mCherry)

Genotyping Quality

Excellent (4 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Close to Inbred (61 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	+	-	-	+	-	-	+	+	-	-	+	-	+	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
multiple C57BL/6 substrains	8911	8460 (99.5%)	1 (0.0%)	42 (0.5%)	408

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
129P2/OlaHsd	43 Clustered	43 (0.5%) 41 Clustered	0 (0.0%) 0 Clustered	0 (0.0%)

Y Chromosome

Y Haplogroup 12 - 100.0% Consistent
Includes B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 14 other strains

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes 129P2/OlaHsd, B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 150 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
C57BL/6J	139	10	156	95.5%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (B6N-Tyr/BrdCrCrl, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac)	5	2	21	33.3%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
multiple C57BL/6 substrains	2857	98.5%	98.4%
129P2/OlaHsd	1	0.0%	0.0%
Heterozygous	42	1.4%	1.6%
Unexplained Homozygous	0	0.0%	0.0%
Unexplained Heterozygous	0	0.0%	0.0%
Total	2900	100.0%	100.0%

Refined Ideogram

Notes

The report is consistent with strain nomenclature and SDS. The presence of bpA, Cre, hCMV_a and b, IRES, and r_FP constructs are detected.