MiniMUGA Sample Report

MiniMUGA Sample Report - Initial Version

Sample ID

B6;FVB-Apoc2em1Arem/Mmnc_M_31714

Neogen ID

PN9205

MMRRC Strain ID

42203

MMRRC Strain Name

B6N;FVB-Apoc2^em1Arem/Mmnc

Summary

The genotype of this sample is of excellent quality. It is male and outbred, and likely a mix of multiple C57BL/6 substrains and multiple FVB/N substrains. Clustering of unexplained markers is evidence of an additional background strain.

Diagnostic SNPs indicate the presence of the background strain groups C57BL/6 and the substrains C57BL/6J, C57BL/6NRj, FVB/NCrl.

No genetic constructs were detected in this sample.

Genotyping Quality

Excellent (7 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Outbred (764 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
multiple C57BL/6 substrains	9719	7515 (81.6%)	941 (10.2%)	750 (8.1%)	513

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
multiple FVB/N substrains	1691 Clustered	1612 (17.5%) 1612 Clustered	63 (0.7%) 59 Clustered	16 (0.2%)

Y Chromosome

Y Haplogroup 12 - 100.0% Consistent
Includes B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 14 other strains

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 151 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
C57BL/6NRj	5	3	20	40.0%
FVB/NCrl	6	4	47	21.3%
C57BL/6J	1	4	156	3.2%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac)	8	2	21	47.6%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
multiple C57BL/6 substrains	1095	39.5%	43.8%
multiple FVB/N substrains	921	33.2%	32.0%
Heterozygous	691	24.9%	22.6%
Unexplained Homozygous	13	0.5%	0.4%
Unexplained Heterozygous	50	1.8%	1.2%
Total	2770	100.0%	100.0%

Refined Ideogram

Notes

The sample is outbred contains diagnostic markers from C57BL/6N, FVB/N, and C57BL/6J. Although there is evidence of contribution from an additional strain.