MiniMUGA Sample Report

MiniMUGA Sample Report - Initial Version

Sample ID

MMRRC_129S(B6)-Stub1tm1Cpat/Mmnc _F26964

Neogen ID

SJ5888

MMRRC Strain ID

37469

MMRRC Strain Name

129S.B6-Stub1^tm1Cpat/Mmnc

Summary

The genotype of this sample is of excellent quality. It is female and inbred, and likely a mix of 129S6/SvEvTac and multiple C57BL/6 substrains.

Diagnostic SNPs indicate the presence of the background strain groups 129S.

The sample contains the following genetic constructs: Bovine growth hormone poly A signal sequence

Genotyping Quality

Excellent (2 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Inbred (2 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
129S6/SvEvTac	9719	9584 (99.9%)	4 (0.0%)	2 (0.0%)	129

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
multiple C57BL/6 substrains	6 Clustered	6 (0.1%) 3 Clustered	0 (0.0%) 0 Clustered	0 (0.0%)

MT Chromosome

MT Haplogroup 6 - 100.0% Consistent
Includes 129S6/SvEvTac and 12 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

Strain Group	Homozygous	Heterozygous	Potential	% Observed
129S (129S1/SvImJ, 129S2/SvHsd, 129S2/SvPasOrlRj, 129S4/SvJaeJ, 129S6/SvEvTac)	9	1	17	58.8%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
129S6/SvEvTac	2914	99.8%	99.7%
multiple C57BL/6 substrains	4	0.1%	0.2%
Heterozygous	2	0.1%	0.1%
Unexplained Homozygous	0	0.0%	0.0%
Unexplained Heterozygous	0	0.0%	0.0%
Total	2920	100.0%	100.0%

Refined Ideogram

Notes

The report is consistent with the SDS. The mouse is inbred for 129S6/SvEvTac at MiniMUGA resolution level. A small region of C57BL/6 markers are present at the site of the targeted mutation on C17.The presence of the bpA constructs is detected.