MiniMUGA Sample Report

MiniMUGA Sample Report - V2

Sample ID

MMRRC_B6;129S4-Myh9tm3(GFP/MYH9/MYH10)Rsad/Mmnc_M30970

Neogen ID

VM4183

MMRRC Strain ID

34322

MMRRC Strain Name

B6;129S4-Myh9^{tm3(GFP/MYH9/MYH10)Rsad}/Mmnc

Summary

The genotype of this sample is of excellent quality. It is male and inbred, and likely a mix of multiple C57BL/6 substrains and 129S4/SvJaeJ.

Diagnostic SNPs indicate the presence of the background strain groups C57BL/6 and the substrains C57BL/6J.

The sample contains the following genetic constructs: Bovine growth hormone poly A signal sequence, "Greenish" Fluorescent Protein (EGFP, EYFP, ECFP)

Genotyping Quality

Excellent (3 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Inbred (44 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
multiple C57BL/6 substrains	8911	8071 (94.9%)	395 (4.6%)	38 (0.4%)	407

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
129S4/SvJaeJ	433 Clustered	433 (5.1%) 422 Clustered	0 (0.0%) 0 Clustered	0 (0.0%)

Y Chromosome

Y Haplogroup 14 - 100.0% Consistent
Includes C57BL/6J and 10 other strains

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 151 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
C57BL/6J	102	4	156	67.9%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (B6N-Tyr/BrdCrCrl, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd)	6	0	21	28.6%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
multiple C57BL/6 substrains	2366	84.5%	87.5%
129S4/SvJaeJ	395	14.1%	11.3%
Heterozygous	38	1.4%	1.2%
Unexplained Homozygous	0	0.0%	0.0%
Unexplained Heterozygous	0	0.0%	0.0%
Total	2799	100.0%	100.0%

Refined Ideogram

Notes

The report is consistent with strain nomenclature and SDS. The presence of GFP and bpA constructs are detected.