MiniMUGA Sample Report

MiniMUGA Sample Report - V2

Sample ID

MMRRC_UNC_M37518

Neogen ID

WD9180

MMRRC Strain ID

42291

MMRRC Strain Name

B6;129-Btbd18^tm1Dean/Mmnc

Summary

The genotype of this sample is of excellent quality. It is male and outbred, and likely a mix of multiple C57BL/6 substrains and 129S4/SvJaeJ.

Diagnostic SNPs indicate the presence of the background strain groups 129S, C57BL/6 and the substrains B6N-Tyr/BrdCrCrl, C57BL/6J.

The sample contains the following genetic constructs: Bovine growth hormone poly A signal sequence

Genotyping Quality

Excellent (6 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Outbred (406 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
multiple C57BL/6 substrains	9719	8704 (94.5%)	116 (1.3%)	387 (4.2%)	512

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
129S4/SvJaeJ	503 Clustered	502 (5.5%) 496 Clustered	0 (0.0%) 0 Clustered	1 (0.0%)

Y Chromosome

Y Haplogroup 6 - 98.4% Consistent
Includes 129S4/SvJaeJ and 11 other strains

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 151 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
B6N-Tyr/BrdCrCrl	10	6	31	51.6%
C57BL/6J	1	2	156	1.9%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac)	2	3	21	23.8%
129S (129S1/SvImJ, 129S2/SvHsd, 129S4/SvJaeJ)	1	2	17	17.6%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
multiple C57BL/6 substrains	2358	82.4%	83.5%
129S4/SvJaeJ	116	4.1%	2.8%
Heterozygous	386	13.5%	13.6%
Unexplained Homozygous	0	0.0%	0.0%
Unexplained Heterozygous	0	0.0%	0.0%
Total	2860	100.0%	100.0%

Refined Ideogram

Notes

The sample is outbred. Strain diagnostics are consistent with strain description. The presence of the BpA construct is detected. The Y chromosome is from 129S.