MiniMUGA Sample Report

MiniMUGA Sample Report - Initial Version

Sample ID

MMRRC_UNC_M36595

Neogen ID

WS5966

MMRRC Strain ID

43541

MMRRC Strain Name

B6;129P2-Dnase1l1^tm1Coy/Mmnc

Summary

The genotype of this sample is of excellent quality. It is male and close to inbred, and likely a mix of multiple C57BL/6 substrains and 129P2/OlaHsd.

Diagnostic SNPs indicate the presence of the background strain groups C57BL/6 and the substrains B6N-Tyr/BrdCrCrl, C57BL/6J.

The sample contains the following genetic constructs: Herpesvirus TK promoter

Genotyping Quality

Excellent (7 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Close to Inbred (83 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
multiple C57BL/6 substrains	9719	9150 (99.4%)	25 (0.3%)	31 (0.3%)	513

Secondary Background
(Autosomes,
X Chromosome)

Strain	Total	Explained	Unexplained	Excluded
129P2/OlaHsd	56 Clustered	56 (0.6%) 54 Clustered	0 (0.0%) 0 Clustered	0 (0.0%)

Y Chromosome

Y Haplogroup 14 - 100.0% Consistent
Includes C57BL/6J and 10 other strains

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes 129P2/OlaHsd, B6N-Tyr/BrdCrCrl, C57BL/6ByJ, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac and 150 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
C57BL/6J	76	38	156	73.1%
B6N-Tyr/BrdCrCrl	1	6	31	22.6%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (C57BL/6J, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NRj, C57BL/6NTac)	3	1	21	19.0%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
multiple C57BL/6 substrains	2877	98.1%	97.5%
129P2/OlaHsd	25	0.9%	1.4%
Heterozygous	31	1.1%	1.2%
Unexplained Homozygous	0	0.0%	0.0%
Unexplained Heterozygous	0	0.0%	0.0%
Total	2933	100.0%	100.0%

Refined Ideogram

Notes

The report is consistent with with strain nomenclature and the SDS. The strain is close to inbred with Chr X fixed for 129P2 around the site of the targeted mutation and small segregating regions on C8 and C9. The presence of the hTK_pr construct is detected