MiniMUGA Sample Report

MiniMUGA Sample Report - Refined Analysis

Sample ID

MMRRC_B6;129S7-Amhr2tm3(cre)Bhr/Mmnc_F31173

Neogen ID

ZX0193

MMRRC Strain ID

14245

MMRRC Strain Name

B6;129S7-Amhr2^tm3(cre)Bhr/Mmnc

Summary

The genotype of this sample is of excellent quality. It is female and inbred, and likely a C57BL/6J mouse.

Diagnostic SNPs indicate the presence of the background strain groups C57BL/6 and the substrains C57BL/6J.

The sample contains the following genetic constructs: Cre recombinase, Internal Ribosome Entry Site

Genotyping Quality

Excellent (20 N calls)
All reported results are dependent on genotyping quality.

Chromosomal Sex

Inbreeding Estimate

Inbred (6 H calls at autosomal, X, and PAR chromosome markers)

Inbreeding and
Genotyping Quality
(Plot)

Constructs Detected

BlastR	bpA	Cas9	chlor	Cre	DTA	g_FP	hCMV_a	hCMV_b	hTK_pr	iCre	IRES	Luc	r_FP	rtTA	SV40	tTA
-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-

Primary Background
(Autosomes,
X Chromosome)

Strain	Total	Consistent	Inconsistent	Heterozygous	Excluded
C57BL/6J	8911	8780 (100.0%)	0 (0.0%)	1 (0.0%)	130

Secondary Background
(Autosomes,
X Chromosome)

Not Applicable

MT Chromosome

MT Haplogroup 1 - 100.0% Consistent
Includes C57BL/6J and 161 other strains

Background Ideogram

Backgrounds Detected
(Diagnostic Alleles)

	Diagnostic Alleles Observed
Substrain	Homozygous	Heterozygous	Potential	% Observed
C57BL/6J	146	3	156	95.5%
Strain Group	Homozygous	Heterozygous	Potential	% Observed
C57BL/6 (B6N-Tyr/BrdCrCrl, C57BL/6J, C57BL/6JBomTac, C57BL/6JEiJ, C57BL/6JOlaHsd)	6	0	21	28.6%

Refined Analysis

Background	Informative Markers	Informative Markers %	Genomic Region %
C57BL/6J	8780	100.0%	100.0%
Heterozygous	0	0.0%	0.0%
Unexplained Homozygous	0	0.0%	0.0%
Unexplained Heterozygous	1	0.0%	0.0%
Total	8781	100.0%	100.0%

Refined Ideogram

Notes

The report is consistent with with strain nomenclature and the SDS. The mouse is congenic C57BL/6J at MiniMUGA resolution level. The presence of Cre and IRES constructs are detected.