1.0Mutant Mouse Resource and Research Centers at UNChttps://www.med.unc.edu/mmrrcDavid Kintonhttps://www.med.unc.edu/mmrrc/author/kinton/rich600338<blockquote class="wp-embedded-content" data-secret="snyuyMEVGr"><a href="https://www.med.unc.edu/mmrrc/resources/embryo-thawing-protocol/">Embryo Thawing Protocol</a></blockquote><iframe sandbox="allow-scripts" security="restricted" src="https://www.med.unc.edu/mmrrc/resources/embryo-thawing-protocol/embed/#?secret=snyuyMEVGr" width="600" height="338" title="“Embryo Thawing Protocol” — Mutant Mouse Resource and Research Centers at UNC" data-secret="snyuyMEVGr" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" class="wp-embedded-content"></iframe><script type="text/javascript"> /* <![CDATA[ */ /*! This file is auto-generated */ !function(d,l){"use strict";l.querySelector&&d.addEventListener&&"undefined"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[^a-zA-Z0-9]/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret="'+t.secret+'"]'),o=l.querySelectorAll('blockquote[data-secret="'+t.secret+'"]'),c=new RegExp("^https?:$","i"),i=0;i<o.length;i++)o[i].style.display="none";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute("style"),"height"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener("message",d.wp.receiveEmbedMessage,!1),l.addEventListener("DOMContentLoaded",function(){for(var e,t,s=l.querySelectorAll("iframe.wp-embedded-content"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute("data-secret"))||(t=Math.random().toString(36).substring(2,12),e.src+="#?secret="+t,e.setAttribute("data-secret",t)),e.contentWindow.postMessage({message:"ready",secret:t},"*")},!1)))}(window,document); /* ]]> */ </script> Samples must be stored in liquid nitrogen (or liquid nitrogen vapor) until ready to thaw Embryo Thawing Using forceps, grasp straw and hold in air for 40 seconds then plunge into room temperature water until ice disappears. Remove from water and gently wipe straw dry. Holding firmly cut off the sealed end midway though air … Read more