Browse by system: Confocal microscopes | Multiphoton system | Widefield microscopes | Computer workstations
Coming soon: tiling/mosaic capabilities on the multiphoton!

Recent additions: A fast and sensitive FV3000RS galvano and resonant scanning confocal, a color and fluorescence MVX10 macroscope, a new brightfield & widefield HCA-compatible Ti2 microscope, and two powerful analysis workstations!

Confocals

Olympus FV3000RS

Inverted microscope with a motorized stage, resonant and galvano scanners, and a stage-top incubator with controlled temperature, CO2 and humidity levels.
Location: Rm. 8104 NRB

Z-stack acquisition | Mosaic imaging | FRAP | Deconvolution | Time-lapse live cell multiposition imaging

Lasers

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405

445 nm
Diode Laser
CFP

488 nm
Diode Laser
FITC, Alexa 488, CY2, GFP, Bodipy

514 nm
Diode laser
YFP

561 nm
Diode laser
Cy3, RFP, Alexa 546, Alexa 561, dsRed2

594 nm
Diode laser
Alexa 594, mCherry

640 nm
Diode laser
Cy5, DRAQ5

Objectives

2x/0.08
Plan Apo
WD = 6.2 mm

4x/0.16
U Plan S-APO
WD = 13 mm

10x/0.40
U PlanS S-Apo DIC
WD = 3.1 mm

10x/0.30
U Plan Fluorite DIC
WD = 10 mm

20x/0.75
U Plan S-Apo DIC
WD = 0.6 mm

20x/0.45
LWD U Plan Flour
WD = 6.6 – 7.8 mm

30x/1.05
U Plan S-ApoN (Silicon oil) DIC
WD = 0.8 mm

60x/1.40
SuperCorr Plan ApoN (oil) DIC
WD = 0.12 mm

Detectors

4 highly-sensitive fluorescence spectral GaAsP detectors
1 transmission
brightfield, DIC


Zeiss LSM 780

inverted confocal microscope with a motorized stage.
Location: Rm. 8122 NRB

Z-stack acquisition | Mosaic imagingFRAPDefinite Focus

Lasers

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405

458/488/514 nm
Multiline Argon laser
458 nm: CFP
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein
514 nm: YFP

561 nm
Diode laser
Cy3, RFP, Alexa 546, Alexa 561, dsRed2

594 nm
Diode laser
Alexa 594, mCherry

633 nm
Helium-Neon laser
Cy5, DRAQ5

Objectives

10x/0.45
Plan-Apochromat
WD = 2.0 mm

20x/0.8
Plan-Apochromat
WD = 0.55 mm

40x/1.3
EC Plan-Neofluar (oil) DIC
WD = 0.21 mm

40x/1.4
Plan-Apochromat (oil) DIC
WD = 0.21 mm

63x/1.40
Plan-Apochromat (oil) DIC
WD = 0.19 mm

Detectors

3 fluorescence
spectral detection: 2 flanking PMTs and the central 34-channel GaAsP detector with ~40% quantum efficiency
1 transmission
brightfield, DIC


Zeiss LSM 710

upright confocal microscope with a motorized stage.
Location: Rm. 8104 NRB

Z-stack acquisition | Mosaic imagingFRAP

Lasers

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405

458/488/514 nm
Multiline Argon laser
458 nm: CFP
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein
514 nm: YFP

543 nm
Helium-Neon laser
Cy3, RFP, Alexa 546, Alexa 561, dsRed2

633 nm
Helium-Neon laser
Cy5, DRAQ5

Objectives

10x/0.45
Plan-Apochromat
WD = 2.0 mm

20x/0.8
Plan-Apochromat
WD = 0.55 mm

40x/1.3
EC Plan-Neofluar (oil) DIC
WD = 0.21 mm

40x/1.4
Plan-Apochromat (oil) DIC
WD = 0.21 mm

63x/1.40
Plan-Apochromat (oil) DIC
WD = 0.19 mm

Detectors

3 fluorescence spectral detectors
1 transmission
brightfield, DIC


Multiphoton microscope

Zeiss LSM 7MP

Fixed stage upright multiphoton microscope optimized for in vivo imaging. Equipped with anaesthetic vaporizer machine.
Location: Rm. 8122 NRB

Z-stack acquisition | Deep imaging

Laser

Coherent Chameleon Vision-S Titanium: Sapphire

Tunable: 690-1050 nm

Repetition: 80 MHz

Pulse width: 75 fs

Objectives

10x/0.3
EC Plan Neofluoar
WD = 5.2 mm

20x/1.0
W-Plan Apo (water)
WD = 1.8 mm

20x/1.0
Clr Plan-Neofluar
(nd = 1.45, CLARITY and FocusClear)
WD = 5.6 mm

20x/1.0
Clr Plan-Apochromat
(nd = 1.38, Scale)
WD = 5.6 mm

63x/1.0
W-PlanApo (water)
WD = 2.1 mm

Detectors

BiG assembly:

2 GaAsP detectors


Widefield microscopes

Olympus MVX10

Upright macroscope with automated scanning for large area mosaic imaging and large field of view for pre- and post-surgical imaging and light-guided surgeries.
Location: Rm. 8104 NRB

Mosaic imaging | Color/Brightfield/Fluorescence imaging | Light-guided surgeries

Filters

No 1, Chroma 89404.
Four color filter set for discrete excitation of DAPI/FITC/TRITC/Cy5 and similar fluorophores with CoolLED pE-300 Ultra illumination.
Ex. 391-32/479-33/554-24/638-31
Em. 434-32/519-26/595-33/697-60
No. 2, Chroma 49002.
Ex. 470/40, Em. 525/50
GFP, Alexa 488, FITC, Cy2
No. 3, Chroma 49004.
Ex. 545/25, Em. 605/70
mCherry, dsRed, Alexa 568, Alexa 594, Cy3, mRFP1, DyLight 594, Mito Tracker 633
 No. 4, Empty.
Use for brightfield imaging.

Objectives

0.63x/0.15
PlanFluor
WD = 87 mm

1x/0.25
PlanFluor
WD = 65 mm

2x/0.5
Plan Fluor
WD = 20 mm

Zoom: 0.63-6.3

Detector

DP80 Dual Color-Monochrome Camera
Data Sheet

Stage

ES111 Prior stage with travel stepper motor and XY joystick


Nikon Eclipse Ti2

Inverted microscope with automated scanning for large area real-time mosaic imaging, High Content (HC) acquisition and analysis and a stage-top incubator with controlled temperature, CO2 and humidity levels for live imaging. Controlled by Nikon Elements software capable of intelligent real-time integration of analysis and acquisition workflows and the High Content Analysis (HCA) module for advanced acquisition and analysis.
Location: Rm. 8122 NRB

Mosaic imaging | HCA imaging and analysis | Live cell imaging | Color/Brightfield/Fluorescence imaging

Filters

No 1., Chroma 49006,
Ex. 350/60, Em. 460/50
DAPI, Hoechst 33258, Alexa 405
No. 2Chroma 49002,
Ex. 470/40, Em. 525/50
GFP, Alexa 488, FITC, Cy2
No. 3, Chroma 49008,
Ex. 560/40, Em. 630/75
mCherry, dsRed, Alexa 568, Alexa 594, Cy3, mRFP1, DyLight 594, Mito Tracker 633
No. 4, Chroma 51005
designed to attenuate the typically bright PI emission and balance it with the emission of FITC.
No. 5. Chroma 49008,
Ex. 620/60, Em. 700/75
Alexa 647, Cy5, Draq5, DyLight 649
 No. 6. Empty, Use for brightfield imaging

Objectives

4x/0.75
PlanFluor
WD = 17.1 mm

10x/0.3
PlanFluor
WD = 16 mm

20x/0.5
Plan Fluor
WD = 2.1 mm

40x/0.75
Plan Fluor
WD = 0.66 mm

Detectors

pco.edge 4.2Q High QE sCMOS camera
Data Sheet

Nikon DS-Fi3 CMOS Color camera
Specifications

Stage

Nikon Ti2-S-SS-E Motorized Stage, XY Stroke: 114mm x 75mm, Repeat Position Accuracy +/- 3μm (or less)


Computer workstations

NRC-Microscopy-Core

A Windows 10 workstation custom-designed for advanced image visualization and processing. It is ideally suited for processing and analysis of large data sets including light sheet imaging data, neuron tracing (semi- and fully automated options), and co-localization and segmentation of cells or subcellular components.
Location: Rm. 8122 NRB

Image Analysis | Deconvolution | Segmentation | Neuron Tracing

Software

Imaris and Imaris Stitcher (Bitplane) – Full License with Support

Olympus FV3000 confocal and cellSens – with Stitching and 3D Deconvolution

Zeiss Zen Blue and Black – with Stitching

Neurolucida 10

Cell Profiler

Fiji/ImageJ

Hardware

12 Core 3.0 GHz CPU

512 GB DDR4-2133 Ram

PNY Quadro P6000 24 GB GPU

2 NVIDIA Tesla K40 12 GB GPUs


NRC-Microscopy-iRODs

A Linux CentOS 7 workstation custom designed for machine learning and multi-threading algorithms. It is ideally suited for running complex programs which typically require cloud computing and is accessible by multiple users simultaneously by remote login..
Location: Rm. 8122 NRB

Image Analysis | 3D Behavior Analysis | High Content Imaging Analysis | Machine Learning

Software

iRODs server

Python

Docker

Singularity

Hardware

14 Core 2.6 GHz CPU

512 GB DDR4-2400 Ram

4 GeForce GTX 1080 Ti 11 GB GPUs