In order to meet the imaging demands of neuroscience investigators, Microscopy Core:

1. Provides technical expertise and state of the art imaging systems to support projects exploring the mechanisms underlying neural development, function, and neurological disorders. Technologies supported include:

  • Confocalmultiphoton and widefield imaging of labeled neural tissue, including automated tiling and optimized imaging instrumentation
  • Automated, in vitro, time-lapse imaging of neurons and glia in brain slices and other ex vivo preparations
  • Tissue clearing and imaging of neural cells and brain structures in whole nervous system.

2. Provides technical expertise and imaging technologies for studying the temporal dynamics of neural development and connectivity in normal and genetic models of neurological disorders in vivo and in whole mount preparations. Technologies supported include:

  • In utero multiphoton imaging methods to visualize the spatiotemporal dynamics of neuronal generation, migration and connectivity in intact, living embryonic brains
  • Multiphoton techniques to visualize structure, activity and connectivity in neural circuits in vivo.

3. Serves as a resource for advanced microscopy training to neuroscience graduate students and post-doctoral fellows in NINDS-funded and other UNC Neuroscience labs. The Core director:

  • Schedules and provides routine hands on training sessions on the use of CMIF microscopes
  • Consults and collaborates on projects that require advanced imaging techniques
  • Organizes and runs a graduate course (NBIO 890-001) on the principles and application of advanced microscopy for neuroscience.