Fees

Service

NINDS and UNC Neuroscience PIs

Other UNC PIs

Outside UNC Projects

Construct design1

$150/hr

$150/hr

$150/hr

Sequence verification2

$20

$20

$20

Recombineering

 

 

 

BAC clone acquisition

Market cost

Market cost

Market cost

Knockout/in or BAC construct

$1000

$2000

$3600

Site-directed mutagenesis

$450

$900

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Plasmid construction3

 

 

 

Simple, one ligation construct

$90

$180

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rAAV construct

$180

$360

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Synthesis DNA

$0.3/bp plus design (up to 3kb)

 

 

ES cell screening

 

 

 

Generate BAC positive control

$100

$200

$500

Genomic Southern (200 clones)4

$1000

$1500

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Genomic Southern (verification)5

$200

$400

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Chromosome Engineering

 

 

 

TALEN (20mer)

Market cost

Market cost

Market cost

TALEN Surveyor Nuclease Assay

$200

$300

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Targeting cassette (< 1kb arms)

$800

$1200

$2000

In situ Hybridization6

 

 

 

Probe

$80

$180

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Slides

$200 (up to 40)

$300

 

Ilumina/HTS library preparation

kit cost + $0 labor / library

kit cost + $100 labor / library

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1Billed if project prematurely terminated by PI.  Not billed if construct completed and purchased.  2Include one primer, one sequencing reaction and analysis of sequence. 3Does not include sequence verification.  4Includes 5’ and 3’ probe preparation, verification that each probe detects correct wild-type band, Southern analysis on up to 200 ES clones with either the 5’ or 3’ probe. 5Includes preparation and probing of one blot containing up to 9 ES clones with one probe (5’, 3’, or internal).6 In situ request will need to add core as a co-author.