Inverted microscope with a motorized stage, resonant and galvano scanners, fluorescence lifetime imaging capabilities, and an available stage-top incubator with controlled temperature, CO2 and humidity levels.

Location: 5115 MEJ

Z-stack acquisition | Mosaic imaging | FRAP | Deconvolution | Time-lapse live cell multiposition imaging | Fluorescence Lifetime Imaging Capabilities

Lasers

Objectives

405 nm
Diode Laser
10x/0.40
Plan Apochromatic
WD = 2.2 mm
440 nm
Diode Laser
Pulsed, SMD
20x/0.75
Plan Apochromatic (multi-immersion)
WD = 0.67 mm
470-670 nm
White Light Laser (WLL2)
Pulse Picker
40x/1.10
Plan Apochromatic (water)
WD = 0.65 mm
40x/1.30
Plan Apochromatic (oil)
WD = 0.24 mm
63x/1.20
Plan Apochromatic (water)
WD = 0.30 mm
63x/1.40
Plan Apochromatic (oil)
WD = 0.14 mm

Detectors

5 fluorescence detectors
2 HyD SP: spectral, highly sensitive, extremely low-noise, hybrid GaAsP detector for time-gated detection;
1 HyD: spectral, highly sensitive hybrid GaAsP detectors;
2 PMT: for more sensitive detection in the blue and far red spectral region
1 Transmitted PMT
brightfield, DIC, polarization

Inverted microscope with a motorized stage, resonant and galvano scanners, and a stage-top incubator with controlled temperature, CO2 and humidity levels.

Location: 5114 MEJ

Z-stack acquisition | Mosaic imaging | FRAP | Deconvolution | Time-lapse live cell multiposition imaging

Lasers

Objectives

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405
2x/0.08
Plan Apochromat
WD = 6.2 mm
445 nm
Diode Laser
CFP
4x/0.16
U Plan S-Apo
WD = 13 mm
488 nm
Diode Laser
FITC, Alexa 488, CY2, GFP, Bodipy
10x/0.30
U Plan Fluorite
WD = 10 mm
514 nm
Diode laser
YFP
10x/0.40
U PlanS S-Apo
WD = 3.1 mm
561 nm
Diode laser
Cy3, RFP, Alexa 546, Alexa 561, dsRed2
20x/0.75
U Plan S-Apo
WD = 0.6 mm
594 nm
Diode laser
Alexa 594, mCherry
20x/0.45
LUC Plan FLN
WD = 6.6 – 7.8 mm
640 nm
Diode laser
Cy5, DRAQ5
30x/1.05
U Plan S-ApoN (Silicon oil)
WD = 0.8 mm
60x/1.40
Plan ApoN (oil)
WD = 0.12 mm

Detectors

4 highly-sensitive fluorescence spectral GaAsP detectors
1 transmission
brightfield, DIC

Inverted confocal microscope with a motorized stage.

Location: 5114 MEJ

Z-stack acquisition | Mosaic imaging | FRAP | Definite Focus

Lasers

Objectives

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405
10x/0.45
Plan-Apochromat
WD = 2.0 mm
458/488/514 nm
Multiline Argon laser
458 nm: CFP
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein
514 nm: YFP
20x/0.8
Plan-Apochromat
WD = 0.55 mm
561 nm
Diode laser
Cy3, RFP, Alexa 546, Alexa 561, dsRed2
40x/1.4
Plan-Apochromat (oil) DIC
WD = 0.13 mm
594 nm
Diode laser
Alexa 594, mCherry
63x/1.40
Plan-Apochromat (oil) DIC
WD = 0.19 mm
633 nm
Helium-Neon laser
Cy5, DRAQ5

Detectors

3 fluorescence
2 flanking PMTs and the central 34-channel GaAsP detector with ~40% quantum efficiency
1 transmission
brightfield, DIC

Upright confocal microscope with a motorized stage.

Location: 7105 MEJ

Z-stack acquisition | Mosaic imaging | FRAP

Lasers

Objectives

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405
10x/0.45
Plan-Apochromat
WD = 2.0 mm
458/488/514 nm
Multiline Argon laser
458 nm: CFP
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein
514 nm: YFP
20x/0.8
Plan-Apochromat
WD = 0.55 mm
543 nm
Helium-Neon laser
Cy3, RFP, Alexa 546, Alexa 561, dsRed2
40x/1.3
EC Plan-Neofluar (oil) DIC
WD = 0.21 mm
633 nm
Helium-Neon laser
Cy5, DRAQ5
40x/1.3
Plan-Apochromat (oil) DIC
WD = 0.21 mm
63x/1.40
Plan-Apochromat (oil) DIC
WD = 0.19 mm

Detectors

3 fluorescence spectral detectors
1 transmission
brightfield, DIC