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Upright macroscope with automated scanning for large area mosaic imaging and large field of view for pre- and post-surgical imaging and light-guided surgeries.

Location: 5115 MEJ

Mosaic imaging | Color/Brightfield/Fluorescence Imaging | Light-guided surgeries

Objectives

Magnification [X] Total Magnification [X] Numerical Aperature (NA)
Working Distance (WD) [mm]
MVPLAPO0.63X (Plan Fluor) 0.63 4.0 – 40 0.15 87
MVPLAPO1X (Plan Fluor) 1 6.3 – 63 0.25 65
MVPLAPO2XC (Plan Fluor) 2 12.5 – 125 0.5 20

 

Filters

Position Excitation (nm) Emission (nm) Dyes
1 391-32/479-33/554-24/638-31 434-32/519-26/595-33/697-60 Chroma 89404
Four color filter set for discrete excitation
of DAPI/FITC/TRITC/Cy5 and similar fluorophores with CoolLED pE-300 Ultra illumination.
2 470/40 525/50 Chroma 49002
GFP, Alexa 488, FITC, Cy2
3 545/25 605/70 Chroma 49004
mCherry, dsRed, Alexa 568, Alexa 594,
Cy3, mRFP1, DyLight 594,
Mito Tracker 633
4 Empty Use for brightfield imaging

 

Detectors

Stage

DP80 Dual Color-Monochrome Camera
Data Sheet
ES111 Prior stage with travel stepper motor and XY joystick

Inverted microscope with automated scanning for large area real-time mosaic imaging, High Content (HC) acquisition and analysis and a stage-top incubator with controlled temperature, CO2 and humidity levels for live imaging. Controlled by Nikon Elements software capable of intelligent real-time integration of analysis and acquisition workflows and the High Content Analysis (HCA) module for advanced acquisition and analysis.

Location: 6113 MEJ
Mosaic imaging | HCA imaging and analysis | Live cell imaging | Color/Brightfield/Fluorescence imaging

Objectives

Magnification [X] Numerical Aperature (NA) Working Distance (WD) [mm]
Plan Fluor 4x 4 0.13 17.1
Plan Apochromat Lambda 4X 4 0.2 20
Plan Fluor 10X 10 0.3 16
Plan Apochromat Lambda 10X 10 0.45 4
Plan Fluor 20X 20 0.5 2.1
S Plan Fluor LWD 20X 20 0.7 1.3-2.3
Plan Fluor 40X 40 0.75 0.66
Plan Apochromat Lambda 40X 40 0.95 0.14

 

Filters

Position Excitation (nm) Emission (nm) Dyes
1 325 – 375 435 – 485 DAPI
2 450 – 490 500 – 550 GFP
3 520 – 580 592.5 – 667.5 TxRed, mCherry
4 625 – 655 665 – 715 Narrow Cy5 (imaging with mCherry, TxRed)
5 590 – 650 667.5 – 737.5 Broad Cy5 (imaging with Alexa 568, Cy3)
6 Empty Use for brightfield imaging

 

Detectors

Stage

pco.edge 4.2Q High QE sCMOS camera
Data Sheet Nikon DS-Fi3 CMOS Color camera
Specifications
Nikon Ti2-S-SS-E Motorized Stage,

XY Stroke: 114mm x 75mm,

Repeat Position Accuracy +/- 3μm (or less)