{"id":2792,"date":"2022-09-20T16:08:17","date_gmt":"2022-09-20T20:08:17","guid":{"rendered":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/?page_id=2792"},"modified":"2024-10-09T14:16:31","modified_gmt":"2024-10-09T18:16:31","slug":"zeiss-lsm-780","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/instrumentation\/zeiss-lsm-780\/","title":{"rendered":"Zeiss LSM 780"},"content":{"rendered":"<div class=\"btn-group btn-group-justified  oscitas-bootstrap-container\"><a class=\"btn btn-default  oscitas-bootstrap-container\" target=\"_self\" href=\"#Specifications\">Hardware Specifications<\/a>\n<a class=\"btn btn-default   oscitas-bootstrap-container\" target=\"_self\" href=\"#Filter_Spectra\">FPbase Filter Spectra<\/a>\n<a class=\"btn btn-default   oscitas-bootstrap-container\" target=\"_blank\" href=\"https:\/\/uncch.ilab.agilent.com\/sc\/4868\/neuroscience-microscopy-core\/?tab=services\">Request a Training<\/a>\n<a class=\"btn btn-default   oscitas-bootstrap-container\" target=\"_blank\" href=\"https:\/\/uncch.ilab.agilent.com\/service_centers\/4868\/equipment_kiosk\/dashboard\">Book in iLab<\/a>\n<\/div>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-2946\" src=\"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-content\/uploads\/sites\/1290\/2022\/09\/LSM-780-cropped-300x300.png\" alt=\"\" width=\"500\" height=\"500\" srcset=\"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-content\/uploads\/sites\/1290\/2022\/09\/LSM-780-cropped-300x300.png 300w, https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-content\/uploads\/sites\/1290\/2022\/09\/LSM-780-cropped-1024x1024.png 1024w, https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-content\/uploads\/sites\/1290\/2022\/09\/LSM-780-cropped-150x150.png 150w, https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-content\/uploads\/sites\/1290\/2022\/09\/LSM-780-cropped-768x768.png 768w, https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-content\/uploads\/sites\/1290\/2022\/09\/LSM-780-cropped-1536x1536.png 1536w, https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-content\/uploads\/sites\/1290\/2022\/09\/LSM-780-cropped-2048x2048.png 2048w, https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-content\/uploads\/sites\/1290\/2022\/09\/LSM-780-cropped-600x600.png 600w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>Inverted microscope with a motorized stage and a stage-top incubator with controlled temperature, CO2 and humidity levels.<\/p>\n<h2>Location: 5114 MEJ<\/h2>\n<h2 style=\"text-align: left\">Modalities<\/h2>\n<ul>\n<li>Z-stack Acquisition<\/li>\n<li>Mosaic Imaging<\/li>\n<li>FRAP<\/li>\n<li>Definite Focus<\/li>\n<li>Time-lapse Live Cell Multiposition Imaging<\/li>\n<\/ul>\n<h2>Specifications<a id=\"Specifications\"><\/a><\/h2>\n<h4><em>Objectives<\/em><\/h4>\n\n<table id=\"tablepress-17\" class=\"tablepress tablepress-id-17 tablepress-responsive\">\n<thead>\n<tr class=\"row-1 odd\">\n\t<th class=\"column-1\">Magnification<\/th><th class=\"column-2\">NA<\/th><th class=\"column-3\">WD (mm)<\/th><th class=\"column-4\">Correction<\/th><th class=\"column-5\">Immersion<\/th><th class=\"column-6\">Notes<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-hover\">\n<tr class=\"row-2 even\">\n\t<td class=\"column-1\">10X<\/td><td class=\"column-2\">0.45<\/td><td class=\"column-3\">2.0<\/td><td class=\"column-4\">Plan Apochromat<\/td><td class=\"column-5\">Air<\/td><td class=\"column-6\"><\/td>\n<\/tr>\n<tr class=\"row-3 odd\">\n\t<td class=\"column-1\">20X<\/td><td class=\"column-2\">0.8<\/td><td class=\"column-3\">0.55<\/td><td class=\"column-4\">Plan Apochromat<\/td><td class=\"column-5\">Air<\/td><td class=\"column-6\"><\/td>\n<\/tr>\n<tr class=\"row-4 even\">\n\t<td class=\"column-1\">40X<\/td><td class=\"column-2\">1.4<\/td><td class=\"column-3\">0.13<\/td><td class=\"column-4\">Plan Apochromat<\/td><td class=\"column-5\">Oil<\/td><td class=\"column-6\"><\/td>\n<\/tr>\n<tr class=\"row-5 odd\">\n\t<td class=\"column-1\">63X<\/td><td class=\"column-2\">1.4<\/td><td class=\"column-3\">0.19<\/td><td class=\"column-4\">Plan Apochromat<\/td><td class=\"column-5\">Oil<\/td><td class=\"column-6\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n<h4><em>Lasers<\/em><\/h4>\n\n<table id=\"tablepress-18\" class=\"tablepress tablepress-id-18 tablepress-responsive\">\n<thead>\n<tr class=\"row-1 odd\">\n\t<th class=\"column-1\">Excitation (nm)<\/th><th class=\"column-2\">Dyes<\/th><th class=\"column-3\">Type<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-hover\">\n<tr class=\"row-2 even\">\n\t<td class=\"column-1\">405<\/td><td class=\"column-2\">DAPI, Hoechst 33258, Alexa 405 <\/td><td class=\"column-3\">Diode<\/td>\n<\/tr>\n<tr class=\"row-3 odd\">\n\t<td class=\"column-1\">458\/488\/514<\/td><td class=\"column-2\">458 nm: CFP <br \/>\n488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein <br \/>\n514 nm: YFP <\/td><td class=\"column-3\">Multiline Argon<\/td>\n<\/tr>\n<tr class=\"row-4 even\">\n\t<td class=\"column-1\">561<\/td><td class=\"column-2\">Cy3, RFP, Alexa 546, Alexa 561, dsRed2 <\/td><td class=\"column-3\">Diode<\/td>\n<\/tr>\n<tr class=\"row-5 odd\">\n\t<td class=\"column-1\">594<\/td><td class=\"column-2\">Alexa 594, mCherry <\/td><td class=\"column-3\">Diode<\/td>\n<\/tr>\n<tr class=\"row-6 even\">\n\t<td class=\"column-1\">633<\/td><td class=\"column-2\">Cy5, DRAQ5 <\/td><td class=\"column-3\">Helium-Neon<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n<h4><em>Detectors<\/em><\/h4>\n\n<table id=\"tablepress-23\" class=\"tablepress tablepress-id-23 tablepress-responsive\">\n<thead>\n<tr class=\"row-1 odd\">\n\t<th class=\"column-1\">Type<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-hover\">\n<tr class=\"row-2 even\">\n\t<td class=\"column-1\">3 fluorescence: <br \/>\n2 flanking PMTS <br \/>\nCentral 34-channel GaAsP with ~40% QE <\/td>\n<\/tr>\n<tr class=\"row-3 odd\">\n\t<td class=\"column-1\">1 transmission: brightfield, DIC<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n<h4><em>Software<\/em><\/h4>\n\n<table id=\"tablepress-24\" class=\"tablepress tablepress-id-24 tablepress-responsive\">\n<thead>\n<tr class=\"row-1 odd\">\n\t<th class=\"column-1\">Version<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-hover\">\n<tr class=\"row-2 even\">\n\t<td class=\"column-1\"><a href=\"https:\/\/www.micro-shop.zeiss.com\/en\/us\/softwarefinder\/software-categories\/zen-black\/#zen-black-module-overview\" target=\"_blank\" rel=\"noopener\">ZEN Black v. 2.3<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n<h2>Filter Spectra<a id=\"Filter_Spectra\"><\/a><\/h2>\n\n<table id=\"tablepress-42\" class=\"tablepress tablepress-id-42 tablepress-responsive\">\n<thead>\n<tr class=\"row-1 odd\">\n\t<th class=\"column-1\">Filter Set<\/th><th class=\"column-2\">Excitation<\/th><th class=\"column-3\">Beamsplitter<\/th><th class=\"column-4\">Emission<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-hover\">\n<tr class=\"row-2 even\">\n\t<td class=\"column-1\"><a href=\"https:\/\/www.micro-shop.zeiss.com\/en\/us\/shop\/filterAssistant\/filtersets\/000000-1114-101\" rel=\"noopener\" target=\"_blank\">Filter Set 43 Cy 3<\/a><\/td><td class=\"column-2\">BP 545\/25<\/td><td class=\"column-3\">FT 570<\/td><td class=\"column-4\">BP 605\/70<\/td>\n<\/tr>\n<tr class=\"row-3 odd\">\n\t<td class=\"column-1\"><a href=\"https:\/\/www.micro-shop.zeiss.com\/en\/us\/shop\/filterAssistant\/filtersets\/488049-9901-000\" rel=\"noopener\" target=\"_blank\">Filter Set 49 DAPI Shift free<\/a><\/td><td class=\"column-2\">G 365<\/td><td class=\"column-3\">FT 395<\/td><td class=\"column-4\">BP 445\/50<\/td>\n<\/tr>\n<tr class=\"row-4 even\">\n\t<td class=\"column-1\"><a href=\"https:\/\/www.micro-shop.zeiss.com\/en\/us\/shop\/filterAssistant\/filtersets\/000000-1031-346\" rel=\"noopener\" target=\"_blank\">Filter Set 38 HE GFP Shift Free<\/a><\/td><td class=\"column-2\">BP 470\/40<\/td><td class=\"column-3\">FT 495<\/td><td class=\"column-4\">BP 525\/50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; Inverted microscope with a motorized stage and a stage-top incubator with controlled temperature, CO2 and humidity levels. Location: 5114 MEJ Modalities Z-stack Acquisition Mosaic Imaging FRAP Definite Focus Time-lapse Live Cell Multiposition Imaging Specifications Objectives Lasers Detectors Software Filter Spectra<\/p>\n","protected":false},"author":111865,"featured_media":0,"parent":2395,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2792","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/pages\/2792","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/users\/111865"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/comments?post=2792"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/pages\/2792\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/pages\/2395"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/media?parent=2792"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}