{"id":3290,"date":"2023-06-15T15:38:21","date_gmt":"2023-06-15T19:38:21","guid":{"rendered":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/?page_id=3290"},"modified":"2023-07-05T15:47:19","modified_gmt":"2023-07-05T19:47:19","slug":"asi-ct-dspim","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/instrumentation\/asi-ct-dspim\/","title":{"rendered":"ASI ct-dSPIM"},"content":{"rendered":"

\"ASI
\nThe dual Selective Plane Illumination Microscope for cleared tissue (ct-dSPIM) is a light sheet microscope configuration that allows for dual views of large samples and is suitable for fast and gentle imaging. Both objectives have a light sheet scanner and camera and the two datasets can be merged to produce an image with isotropic resolution.<\/p>\n

Location:<\/strong> 5114 MEJ<\/h2>\n

Training and Usage:\u00a0<\/strong><\/h2>\n

Details coming soon!<\/p>\n

Acknowledgements<\/strong><\/h2>\n

We suggest using the following language if the ASI ct-dSPIM was used in acquiring your images and data:<\/p>\n

“The ASI ct-dSPIM microscope was funded with support from NIH grant S10 MH130455.”<\/span><\/em><\/p>\n

Specifications:<\/strong><\/h2>\n

Objectives<\/em><\/h4>\n\n\n\n\n\t\n\n\t\n\t
Magnification<\/th>NA<\/th>WD (mm)<\/th>Immersion Media<\/th>\n<\/tr>\n<\/thead>\n
<\/td>0.4 @ 1.45 RI<\/td>12<\/td>Multi-immersion<\/td>\n<\/tr>\n
<\/td>0.7 @ 1.45 RI<\/td>10<\/td>Multi-immersion<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n

Lasers<\/em><\/h4>\n

Oxxius 488, 561, 638, 751 nm<\/p>\n

Detectors<\/em><\/h4>\n

ORCA Fusion back-thinned Gen-III <\/span>sCMOS<\/span> camera<\/span><\/a><\/p>\n

Software<\/em><\/h4>\n

Micro-Manager<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

The dual Selective Plane Illumination Microscope for cleared tissue (ct-dSPIM) is a light sheet microscope configuration that allows for dual views of large samples and is suitable for fast and gentle imaging. Both objectives have a light sheet scanner and camera and the two datasets can be merged to produce an image with isotropic resolution. … Read more<\/a><\/p>\n","protected":false},"author":111865,"featured_media":0,"parent":2395,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-3290","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/pages\/3290","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/users\/111865"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/comments?post=3290"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/pages\/3290\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/pages\/2395"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/neuroscience\/neuro-microscopy\/wp-json\/wp\/v2\/media?parent=3290"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}